Invitrogen

CD45RA Monoclonal Antibody (MEM-56), Qdot™ 655

CD45RA Antibody in Flow Cytometry (Flow)

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CD45RA Antibody (Q10069) in Flow

Qdot® 655 anti-human CD45RA conjugate 655/20 band pass filter

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Product Details

Q10069

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 9 publications 9 publications

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

MEM-56

Immunogen

Human CD45RA

Conjugate

Qdot™ 655 Qdot™ 655 Qdot™ 655

Excitation/Emission Max

300/654 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

0.05M borate, pH 8.3, with 1M betaine

Contains

0.05% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2556451

Product Specific Information

Qdot™ Antibody (Ab) conjugates possess a bright fluorescence emission that makes them well suited for the detection of low-abundance extracellular proteins. Approximately the same size as R-phycoerythrin (R-PE) and compatible with existing organic fluorophore conjugates, Qdot™ Ab conjugates can be excited with any wavelength below their emission maximum, but are best excited by UV or violet light. The narrow, symmetric emission profiles of Qdot Ab conjugates allow for minimal compensation when using a single excitation source, and the very long stoke shifts enable better, more efficient multicolor assays using the 405 nm violet laser. Available in multiple colors for use in flow cytometry, these advantages make Qdot Ab conjugates powerful tools for antibody labeling and staining. Staining: Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA). We recommend analysis of cells within 18 hours of staining. If dilute reagent is used, dilute only the quantity of reagent to be used within one day. Qdot Ab conjugates may be mixed with other antibodies, but use the diluted conjugates on the day of dilution. Qdot Ab conjugates can be used for surface staining applications with most conventional sample preparation reagents, such as Cal-Lyse™ Lysing Solution and FIX & PERM™ reagents, with minimal effect on fluorescence. We have observed some batches of BD FACS™ Lysing Solution to interfere with Qdot Ab conjugate fluorescence. Each lot has been tested by flow cytometry using human peripheral blood leukocytes. The isotype control for this antibody is mouse IgG2a, Cat. No. Q10015.

Instrument setup: Qdot Ab conjugates are excited optimally with UV or 405 nm light, although excitation can be obtained with any wavelength below the emission maximum of a given Qdot™ nanocrystal, such as with a 488-nm laser. Qdot Ab conjugates can be used on cytometers that do not have UV or violet excitation sources as long as they have appropriate emission filters. Make sure the cytometer has an appropriate emission filter for the Qdot Ab conjugate being used; alternate filters can be used as long as they capture the emission maximum, but filter width impacts spectral overlap corrections. And be sure to check for Qdot Ab conjugate emission in any channel that can capture nanocrystal emission off of other lasers on the cytometer. For Cat. No. Q10069: peak excitation 405 (488) nm/peak emission 655 nm; recommended filter 655/20 nm.

Store reagents at 2-8°C in the dark. Do not freeze. Because Qdot nanocrystals are conjugated to biological materials, some loss of activity may be observed with prolonged storage. When stored as instructed, expires six months from date of receipt unless otherwise indicated on product label. Qdot Ab conjugates are photostable, and do not need to be protected from light. However, if using Qdot Ab conjugates in combination with conventional fluorochrome conjugated antibodies, minimize light exposure during handling, incubation with cells, and prior to analysis. The Qdot Ab conjugates contain cadmium and selenium in an inorganic crystalline form. Dispose of the material in compliance with all applicable local, state, and federal regulations for disposal of these classes of material. For more information on the composition of these materials, consult the Safety Data Sheets (SDSs).

Target Information

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Proteomics of immune cells from liver tumors reveals immunotherapy targets.

Cell genomics

Canale FP,Neumann J,von Renesse J,Loggi E,Pecoraro M,Vogel I,Zoppi G,Antonini G,Wolf T,Jin W,Zheng X,La Barba G,Birgin E,Forkel M,Nilsson T,Marone R,Mueller H,Pelletier N,Jeker LT,Civenni G,Schlapbach C,Catapano CV,Seifert L,Seifert AM,Gillessen S,De Dosso S,Cristaudi A,Rahbari NN,Ercolani G,Geiger R

Q10069 was used in Flow Cytometry to profile proteomes of cancer tissue as well as monocyte/macrophages, CD4+ and CD8+ T cells, and NK cells isolated from tumors, liver, and blood of 48 patients with hepatocellular carcinoma.

Wed Jun 14 00:00:00 UTC 2023

Broadly reactive human CD4⁺ T cells against Enterobacteriaceae are found in the naïve repertoire and are clonally expanded in the memory repertoire.

European journal of immunology

Cassotta A,Goldstein JD,Durini G,Jarrossay D,Baggi Menozzi F,Venditti M,Russo A,Falcone M,Lanzavecchia A,Gagliardi MC,Latorre D,Sallusto F

Q10069 was used in Flow cytometry/Cell sorting to demonstrate that a large fraction of memory Th cell clones was broadly cross-reactive to several Enterobacteriaceae species.

Mon Mar 01 00:00:00 UTC 2021

Deciphering and predicting CD4+ T cell immunodominance of influenza virus hemagglutinin.

The Journal of experimental medicine

Cassotta A,Paparoditis P,Geiger R,Mettu RR,Landry SJ,Donati A,Benevento M,Foglierini M,Lewis DJM,Lanzavecchia A,Sallusto F

Q10069 was used in Flow cytometry/Cell sorting to show the presence of a broad repertoire of naive T cells specific for cryptic H1-HA peptides and demonstrate that antigen processing represents a major constrain determining immunodominance.

Mon Oct 05 00:00:00 UTC 2020

T Cell Repertoire Maturation Induced by Persistent and Latent Viral Infection Is Insufficient to Induce Costimulation Blockade Resistant Organ Allograft Rejection in Mice.

Frontiers in immunology

Espinosa JR,Mou D,Adams BW,DiBernardo LR,MacDonald AL,McRae M,Miller AN,Song M,Stempora LL,Wang J,Iwakoshi NN,Kirk AD

Q10069 was used in Flow cytometry/Cell sorting to investigate whether costimulation blockade-resistant rejection would emerge in a model of virus-induced memory differentiation designed to more closely mimic clinical conditions of organ allograft rejection.

Wed Nov 20 00:00:00 UTC 2019

Detailed characterization of human Mycobacterium tuberculosis specific HLA-E restricted CD8⁺ T cells.

European journal of immunology

Prezzemolo T,van Meijgaarden KE,Franken KLMC,Caccamo N,Dieli F,Ottenhoff THM,Joosten SA

Q10069 was used in Flow cytometry/Cell sorting to characterise human Mycobacterium tuberculosis specific HLA-E restricted CD8+ T cells.

Thu Feb 01 00:00:00 UTC 2018

The Transcription Factor Hobit Identifies Human Cytotoxic CD4⁺ T Cells.

Frontiers in immunology

Oja AE,Vieira Braga FA,Remmerswaal EB,Kragten NA,Hertoghs KM,Zuo J,Moss PA,van Lier RA,van Gisbergen KP,Hombrink P

Q10069 was used in Flow cytometry/Cell sorting to suggest a shared differentiation pathway in CD4+, CD8+, and γδ+ T cells that may involve Hobit-driven acquisition of long-lived cytotoxic effector function.

Mon Jan 31 00:00:00 UTC 2022

Strain-specific Plasmodium falciparum multifunctional CD4(+) T cell cytokine expression in Malian children immunized with the FMP2.1/AS02A vaccine candidate.

Vaccine

Graves SF,Kouriba B,Diarra I,Daou M,Niangaly A,Coulibaly D,Keita Y,Laurens MB,Berry AA,Vekemans J,Ripley Ballou W,Lanar DE,Dutta S,Gray Heppner D,Soisson L,Diggs CL,Thera MA,Doumbo OK,Plowe CV,Sztein MB,Lyke KE

Q10069 was used in flow cytometry to characterize the immune responses of children immunized with Plasmodium falciparum apical membrane antigen 1

Tue May 17 00:00:00 UTC 2016

Defining human dendritic cell progenitors by multiparametric flow cytometry.

Nature protocols

Breton G,Lee J,Liu K,Nussenzweig MC

Q10069 was used in Flow Cytometry to assess the in vitro differentiation and proliferation of DC progenitors.

Tue Sep 01 00:00:00 UTC 2015

Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.

PloS one

Deng N,Weaver JM,Mosmann TR

Q10069 was used in flow cytometry to investigate the anti-influenza responses of T cell subsets

Thu Dec 18 00:00:00 UTC 2014

Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.

Vaccine

Li X,Miao H,Henn A,Topham DJ,Wu H,Zand MS,Mosmann TR

Q10069 was used in flow cytometry to study influenza-specific CD4 T cells respond to vaccination using Ki67 as a marker.

Fri Jun 29 00:00:00 UTC 2012

CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses.

Vaccine

Scheible K,Zhang G,Baer J,Azadniv M,Lambert K,Pryhuber G,Treanor JJ,Topham DJ

Q10069 was used in flow cytometry to examine human cross-reactive T cells against a pandemic virus.

Thu Mar 03 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Ly-5; Lyt-4; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T100; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45RA Monoclonal Antibody (MEM-56), Qdot™ 655

CD45RA Antibody (Q10069) in Flow

Product Details

Q10069

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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