Invitrogen

CD45RB Monoclonal Antibody (PD7/26), Alexa Fluor™ 488, eBioscience™

CD45RB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

CD45RB Antibody (53-9458-82) in IHC (P)

Immunohistochemical analysis of CD45RB was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Fo... View More

CD45RB Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

53-9458-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-Dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

PD7/26

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2574449

Product Specific Information

Description: The monoclonal antibody PD7/26 recognizes CD45RB, which is an isoform of the Leukocyte Common Antigen (LCA) also known as CD45. CD45RB is a protein tyrosine phosphatase receptor that is present on most hematopoietic cells and absent on non-hematopoietic cells. Expression of CD45RB is more restricted than CD45 and can only be found on T cell subsets, B, NK, and myeloid cells. Regulatory T cells (Tregs) have been show to express low levels of CD45RB, which may correlate with increased migration to sites of infection. In contrast, high CD45RB expression results in IL-2 and IFN gamma production instead of IL-4 in vitro.

Applications Reported: This PD7/26 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.

Applications Tested: This PD7/26 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RB is a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed in microglia and inflammatory cells. In addition to involvement in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

DOG1 as a novel antibody-drug conjugate target for the treatment of multiple gastrointestinal tumors and liver metastasis.

Frontiers in immunology

Wu Y,Li W,Chen X,Wang H,Su S,Xu Y,Deng X,Yang T,Wei M,Li L,Liu Y,Yang J,Li W

Published figure using CD45RB monoclonal antibody (Product # 53-9458-82) in Flow Cytometry

Tue Feb 14 00:00:00 UTC 2023

DOCK8-expressing T follicular helper cells newly generated beyond self-organized criticality cause systemic lupus erythematosus.

iScience

Shiozawa S,Tsumiyama K,Miyazaki Y,Uto K,Sakurai K,Nakashima T,Matsuyama H,Doi A,Tarui M,Izumikawa M,Kimura M,Fujita Y,Satonaka C,Horiuchi T,Matsubara T,Oribe M,Yamane T,Kagawa H,Li QZ,Mizuno K,Mukai Y,Murakami K,Enya T,Tsukimoto S,Hakata Y,Miyazawa M,Shiozawa K

53-9458-82 was used in Flow Cytometry to study the induction of systemic autoimmunity in mice normally not prone to autoimmune disease by repeated stimulation with an antigen.

Fri Jan 21 00:00:00 UTC 2022

Ovarian Cancer Cells in Ascites Form Aggregates That Display a Hybrid Epithelial-Mesenchymal Phenotype and Allows Survival and Proliferation of Metastasizing Cells.

International journal of molecular sciences

Capellero S,Erriquez J,Battistini C,Porporato R,Scotto G,Borella F,Di Renzo MF,Valabrega G,Olivero M

53-9458-82 was used in Immunocytochemistry-immunoflourescence to show that metastasizing units display a hybrid phenotype that allows maintenance of the 3D structures and the plasticity necessary for implant and seeding into peritoneal lining.

Thu Jan 13 00:00:00 UTC 2022

Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging.

Nature protocols

Du Z,Lin JR,Rashid R,Maliga Z,Wang S,Aster JC,Izar B,Sorger PK,Santagata S

53-9458-82 was used in Immunocytochemistry to demonstrate assembly of a 16-antibody panel for enumerating and localizing T cells and B cells, macrophages, and cells expressing immune checkpoint regulators.

Tue Oct 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

View more View less

Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

View more View less

UniProt ID: (Human) P08575

View more View less

Entrez Gene ID: (Human) 5788

View more View less

Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD45RB Monoclonal Antibody (PD7/26), Alexa Fluor™ 488, eBioscience™

CD45RB Antibody (53-9458-82) in IHC (P)

Product Details

53-9458-82

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!