Invitrogen

CD45RO Monoclonal Antibody (UCHL1), eBioscience™

CD45RO Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD45RO Antibody (14-0457-82) in IHC (P)

Immunohistochemical analysis of CD45RO was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Fol... View More

CD45RO Antibody in Flow Cytometry (Flow)

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Product Details

14-0457-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Flow Cytometry (Flow)

0.5 µg/test

View 14 publications 14 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Bovine, Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

UCHL1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_467270

Product Specific Information

Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.

Applications Reported: The UCHL1 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested: The UCHL1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Soluble NKG2D ligands impair CD8⁺ T cell antitumor function dependent of NKG2D downregulation in neuroblastoma.

Oncology letters

Zhang Y,Luo F,Dong K

Published figure using CD45RO monoclonal antibody (Product # 14-0457-82) in Flow Cytometry

Sat Jul 01 00:00:00 UTC 2023

Longitudinal analysis of subtype C envelope tropism for memory CD4⁺ T cell subsets over the first 3 years of untreated HIV-1 infection.

Retrovirology

Gartner MJ,Gorry PR,Tumpach C,Zhou J,Dantanarayana A,Chang JJ,Angelovich TA,Ellenberg P,Laumaea AE,Nonyane M,Moore PL,Lewin SR,Churchill MJ,Flynn JK,Roche M

Published figure using CD45RO monoclonal antibody (Product # 14-0457-82) in Flow Cytometry

Thu Aug 06 00:00:00 UTC 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using CD45RO monoclonal antibody (Product # 14-0457-82) in Flow Cytometry

Tue Nov 17 00:00:00 UTC 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD45RO monoclonal antibody (Product # 14-0457-82) in Flow Cytometry

Wed Jul 14 00:00:00 UTC 2021

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD45RO monoclonal antibody (Product # 14-0457-82) in Flow Cytometry

Sat Apr 01 00:00:00 UTC 2017

TET-catalyzed oxidation of intragenic 5-methylcytosine regulates CTCF-dependent alternative splicing.

The EMBO journal

Marina RJ,Sturgill D,Bailly MA,Thenoz M,Varma G,Prigge MF,Nanan KK,Shukla S,Haque N,Oberdoerffer S

14-0457 was used in Flow cytometry/Cell sorting to expand our current concept of the pre-mRNA "splicing code" to include dynamic intragenic DNA methylation catalyzed by the TET proteins.

Mon Feb 01 00:00:00 UTC 2016

Efficient generation of antigen-specific CTLs by the BAFF-activated human B Lymphocytes as APCs: a novel approach for immunotherapy.

Oncotarget

Yiwen Z,Shilin G,Yingshi C,Lishi S,Baohong L,Chao L,Linghua L,Ting P,Hui Z

14-0457 was used in Flow cytometry/Cell sorting to expand human B-lymphocytes on a large scale while maintaining their antigen-presenting ability by using both CD40L and B-cell activating factor (BAFF).

Tue Nov 22 00:00:00 UTC 2016

Aging influences the response of T cells to stimulation by the ellagitannin, oenothein B.

International immunopharmacology

Ramstead AG,Schepetkin IA,Todd K,Loeffelholz J,Berardinelli JG,Quinn MT,Jutila MA

14-0457 was used in Flow cytometry/Cell sorting to examine oenothein B responsiveness in T cells from cord blood, young, and adult donors.

Mon Jun 01 00:00:00 UTC 2015

Aging influences the response of T cells to stimulation by the ellagitannin, oenothein B.

International immunopharmacology

Ramstead AG,Schepetkin IA,Todd K,Loeffelholz J,Berardinelli JG,Quinn MT,Jutila MA

14-0457 was used in Flow cytometry/Cell sorting to examine oenothein B responsiveness in T cells from cord blood, young, and adult donors.

Mon Jun 01 00:00:00 UTC 2015

Defining CD4 T cell memory by the epigenetic landscape of CpG DNA methylation.

Journal of immunology (Baltimore, Md. : 1950)

Komori HK,Hart T,LaMere SA,Chew PV,Salomon DR

14-0457 was used in Flow cytometry/Cell sorting to study differences in the methylation status of genes in memory and naive T cells.

Sun Feb 15 00:00:00 UTC 2015

Functional expression of CD137 (4-1BB) on T helper follicular cells.

Oncoimmunology

Alfaro C,Echeveste JI,Rodriguez-Ruiz ME,Solorzano JL,Perez-Gracia JL,Idoate MA,Lopez-Picazo JM,Sanchez-Paulete AR,Labiano S,Rouzaut A,Oñate C,Aznar A,Lozano MD,Melero I

Published figure using CD45RO monoclonal antibody (Product # 14-0457-82) in Flow Cytometry

Tue Dec 01 00:00:00 UTC 2015

Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Hartmann N,Giese NA,Giese T,Poschke I,Offringa R,Werner J,Ryschich E

14-0457 was used in Flow cytometry/Cell sorting to analyse how chemokines and extracellular matrix collagen interact in mediating T-cell infiltration in pancreatic ductal adenocarcinoma.

Tue Jul 01 00:00:00 UTC 2014

CD39 and CD161 modulate Th17 responses in Crohn's disease.

Journal of immunology (Baltimore, Md. : 1950)

Bai A,Moss A,Kokkotou E,Usheva A,Sun X,Cheifetz A,Zheng Y,Longhi MS,Gao W,Wu Y,Robson SC

14-0457 was used in Flow cytometry/Cell sorting to report that coexpression of CD161 and CD39 not only identifies these cells but also promotes Th17 generation.

Wed Oct 01 00:00:00 UTC 2014

Neutralizing IL-6 reduces human arterial allograft rejection by allowing emergence of CD161+ CD4+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Fogal B,Yi T,Wang C,Rao DA,Lebastchi A,Kulkarni S,Tellides G,Pober JS

14-0457 was used in Immunohistochemistry to study the role of IL-6 in allogeneic T cell infiltration and intimal expansion in a model of human allograft rejection.

Thu Dec 15 00:00:00 UTC 2011

Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells.

The Journal of experimental medicine

Poulin LF,Salio M,Griessinger E,Anjos-Afonso F,Craciun L,Chen JL,Keller AM,Joffre O,Zelenay S,Nye E,Le Moine A,Faure F,Donckier V,Sancho D,Cerundolo V,Bonnet D,Reis e Sousa C

14-0457 was used in Flow cytometry/Cell sorting to investigate a population of human DCs that expresses DNGR-1 and high levels of BDCA3.

Mon Jun 07 00:00:00 UTC 2010

Expression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung.

Respiratory research

Kawamata N,Xu B,Nishijima H,Aoyama K,Kusumoto M,Takeuchi T,Tei C,Michie SA,Matsuyama T

14-0457 was used in Immunohistochemistry to identify which adhesion molecules recruit specific subsets of lymphocytes into human bronchus-associated lymphoid tissue.

Thu Oct 22 00:00:00 UTC 2009

TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses.

Molecular immunology

Arimura Y,Vang T,Tautz L,Williams S,Mustelin T

14-0457 was used in Magnetic cell separation to investigate how PTP-PEST is down-regulated following T cell activation, and how that can lead to reduced IL-2 secretion following TCR and CD28 stimulation.

Sun Jun 01 00:00:00 UTC 2008

Interaction of human PD-L1 and B7-1.

Molecular immunology

Butte MJ,Peña-Cruz V,Kim MJ,Freeman GJ,Sharpe AH

14-0457 was used in Flow cytometry/Cell sorting to investigate whether human B7-1 and PD-L1 can participate in a similar interaction as murine B7-1 and PD-L1 do, showing that there is interaction of human PD-L1 and B7-1.

Fri Aug 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45RO Monoclonal Antibody (UCHL1), eBioscience™

CD45RO Antibody (14-0457-82) in IHC (P)

Product Details

14-0457-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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