Invitrogen

CD47 Monoclonal Antibody (B6H12.2)

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CD47 Antibody (MA5-11895) in WB

Western blot analysis of CD47 was performed by loading 25 µg of human placenta (lane 1), Jurkat (lane 2) and BAF-3 (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a CD47 monoclonal antibody (Product # MA5-11895) at a dilution of 1:50 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western B... View More

CD47 Antibody in Immunocytochemistry (ICC/IF)

CD47 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD47 Antibody — Fig. 5 Effects of GrA on the expression and distribution of pancreatic CSC markers. a Protein expression profiles of CD133 and CD44 in BxPC-3 and MIA PaCa-2 cells treated with 0.1, 0.01 and 0.001 muM of GrA for 48 h represented graphically. b Densitometry quantitation of CD44 band intensities in western blot. Cells were treated with GrA for 24 h and 48 h, respectively. c Immunofluorescence staining images showed CD47 distribution in BxPC-3 cells treated with 0.05 muM GrA for 24 h. d Immunofluorescence staining images showed CD47 distribution in MIA PaCa-2 cells treated with 0.05 muM GrA for 24 h. Cellular nucleus was stained by DAPI exhibiting blue fluorescence while CD47 on cell surface was marked by antibodies with green fluorescence. The white arrow indicates the CD47 cluster {TM}

Product Details

MA5-11895

Applications

Tested Dilution

Publications

Western Blot (WB)

1:10-1:100

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Flow Cytometry (Flow)

0.5 µg/test

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mL

Neutralization (Neu)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

B6H12.2

Immunogen

Intact CD47 purified from placenta

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11009368

Product Specific Information

MA5-11895 targets CD47 in WB, FACS, IF, and IP applications and shows reactivity with Human samples.

The MA5-11895 immunogen is intact CD47 purified from placenta.

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Osteosarcoma-enriched transcripts paradoxically generate osteosarcoma-suppressing extracellular proteins.

eLife

Li K,Huo Q,Dimmitt NH,Qu G,Bao J,Pandya PH,Saadatzadeh MR,Bijangi-Vishehsaraei K,Kacena MA,Pollok KE,Lin CC,Li BY,Yokota H

MA5-11895 was used in Western Blotting to support the possibility of employing a paradoxical strategy of utilizing Osteosarcoma transcriptomes for the treatment of Osteosarcoma.

Tue Mar 21 00:00:00 UTC 2023

CD47 Promotes Autoimmune Valvular Carditis by Impairing Macrophage Efferocytosis and Enhancing Cytokine Production.

Journal of immunology (Baltimore, Md. : 1950)

Meier LA,Faragher JL,Osinski V,Auger JL,Voeller R,Marath A,Binstadt BA

MA5-11895 was used in Immunocytochemistry-immunofluorescence to highlight the CD47:SIRPα interaction as a key driver of chronic cardiac valve inflammation and suggest these molecules as potential therapeutic targets to reduce cardiovascular disease risk in autoantibody-driven inflammatory diseases.

Wed Jun 15 00:00:00 UTC 2022

Oxidation of Innate Immune Checkpoint CD47 on Cancer Cells with Non-Thermal Plasma.

Cancers

Lin A,Razzokov J,Verswyvel H,Privat-Maldonado A,De Backer J,Yusupov M,Cardenas De La Hoz E,Ponsaerts P,Smits E,Bogaerts A

Published figure using CD47 monoclonal antibody (Product # MA5-11895) in Immunohistochemistry

Tue Feb 02 00:00:00 UTC 2021

Leishmania donovani Induces Autophagy in Human Blood-Derived Neutrophils.

Journal of immunology (Baltimore, Md. : 1950)

Pitale DM,Gendalur NS,Descoteaux A,Shaha C

MA5-11895 was used in Immunocytochemistry to demonstrate how Leishmania-induced human polymorphonuclear neutrophil (hPMN) autophagy regulates the silent mode of parasite transfer to macrophages by influencing the engulfment of infected cells.

Fri Feb 15 00:00:00 UTC 2019

The ionophore antibiotic gramicidin A inhibits pancreatic cancer stem cells associated with CD47 down-regulation.

Cancer cell international

Wang RQ,Geng J,Sheng WJ,Liu XJ,Jiang M,Zhen YS

MA5-11895 was used in Western Blotting to study the effect of Gramicidin A on pancreatic cancer stem cells and determine the mechanism of action.

Fri Apr 08 00:00:00 UTC 2022

The "don't eat me" signal CD47 is a novel diagnostic biomarker and potential therapeutic target for diffuse malignant mesothelioma.

Oncoimmunology

Schürch CM,Forster S,Brühl F,Yang SH,Felley-Bosco E,Hewer E

MA5-11895 was used in Flow Cytometry to demonstrate the upregulation of signalling molecule CD47 in diffuse malignant mesothelioma cells, and highlight its potential as a diagnostic tool and therapeutic target.

Tue Jan 12 00:00:00 UTC 2021

A cytokine-controlled mechanism for integrated regulation of T-lymphocyte motility, adhesion and activation.

Immunology

Bergström SE,Bergdahl E,Sundqvist KG

MA5-11895 was used in blocking or activating experiment and western blot to study the mechanism underlying cytokine-mediated modulation of the motility, adhesion and activation of T-cells

Sun Dec 01 00:00:00 UTC 2013

Latency associated peptide has in vitro and in vivo immune effects independent of TGF-beta1.

PloS one

Ali NA,Gaughan AA,Orosz CG,Baran CP,McMaken S,Wang Y,Eubank TD,Hunter M,Lichtenberger FJ,Flavahan NA,Lawler J,Marsh CB

MA5-11895 was used in blocking or activating experiment to study the TGF-beta1-independent immune effects of latency associated peptide

Wed Apr 02 00:00:00 UTC 2008

Erythrocyte adhesion is modified by alterations in cellular tonicity and volume.

British journal of haematology

Wandersee NJ,Punzalan RC,Rettig MP,Kennedy MD,Pajewski NM,Sabina RL,Paul Scott J,Low PS,Hillery CA

MA5-11895 was used in blocking or activating experiment to study the effect of cellular tonicity and volume on erythrocyte adhesion

Tue Nov 01 00:00:00 UTC 2005

Increased erythrocyte adhesion in mice and humans with hereditary spherocytosis and hereditary elliptocytosis.

Blood

Wandersee NJ,Olson SC,Holzhauer SL,Hoffmann RG,Barker JE,Hillery CA

MA5-11895 was used in blocking or activating experiment to study multiorgan thrombosis and infarction in mice and humans with hereditary spherocytosis and hereditary elliptocytosis

Thu Jan 15 00:00:00 UTC 2004

The C-terminal 26-residue peptide of serpin A1 stimulates proliferation of breast and liver cancer cells: role of protein kinase C and CD47.

FEBS letters

Congote LF,Temmel N

MA5-11895 was used in blocking or activating experiment to study the mechanism by which the C-terminal peptide of serpin A1 stimulates proliferation of breast and liver cancer cells

Fri Oct 22 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody 1D8; Antigenic surface determinant protein OA3; CD47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; Leukocyte surface antigen CD47; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47

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Gene Aliases: CD47; IAP; MER6; OA3

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UniProt ID: (Human) Q08722

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Entrez Gene ID: (Human) 961

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Function(s)

protein binding thrombospondin receptor activity

Process(es)

cell adhesion integrin-mediated signaling pathway opsonization positive regulation of cell proliferation response to bacterium positive regulation of cell-cell adhesion extracellular matrix organization positive regulation of inflammatory response positive regulation of phagocytosis positive regulation of T cell activation leukocyte migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD47 Monoclonal Antibody (B6H12.2)

CD47 Antibody (MA5-11895) in WB

Product Details

MA5-11895

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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