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CD47 Antibody (MA1-10239) in Flow
Flow Cytometry analysis of CD47 using CD47 Monoclonal Antibody (MEM-122), FITC (Product # MA1-10239). Separation of human CD47 positive lymphocytes (red-filled) from CD47 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD47 (MEM-122) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
Product Details
MA1-10239
Product Specifications
Species Reactivity
Human,
Non-human primate,
Pig
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
MEM-122
Immunogen
COS-7 (African green monkey) cells
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
TBS, pH 8, with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11155223
Product Specific Information
This antibody reacts with an extracellular epitope of CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
Target Information
CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antigen identified by monoclonal antibody 1D8; Antigenic surface determinant protein OA3; CD47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; Leukocyte surface antigen CD47; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47
Gene Aliases: CD47; CD47/IAP; IAP; MER6; OA3
UniProt ID: (Human) Q08722, (Pig) Q9GKE8
Entrez Gene ID: (Human) 961, (Pig) 397042
cell adhesion
integrin-mediated signaling pathway
opsonization
positive regulation of cell proliferation
response to bacterium
positive regulation of cell-cell adhesion
extracellular matrix organization
positive regulation of inflammatory response
positive regulation of phagocytosis
positive regulation of T cell activation
leukocyte migration
Performance Guarantee
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