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Invitrogen
CD47 Chimeric Recombinant Rabbit Monoclonal Antibody (h4C1)
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FIGURE: 1 / 2
CD47 Chimeric Antibody (MA5-51747) in ICC/IF
Immunocytochemistry analysis of CD47 in paraformaldehyde fixed Jurkat cells using CD47 Chimeric Recombinant Rabbit Monoclocal Antibody (Product # MA5-51747) at a 1:100 dilution for 1h followed by Alexa Fluor 488 secondary antibody (1:1,000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: primary antibody, DAPI, merged channels and an isotype co... View More
Product Details
MA5-51747
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
h4C1
Immunogen
The original mouse antibody was generated by immunizing BALB/c mice with human CD47-ecto recombinant protein emulsified with complete Freund's adjuvant. Later on the original mouse antibody was used to generate the humanized version of the antibody using sequence homology and computational prediction.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3250439
Product Specific Information
This antibody binds the FG loop of the human CD47, which interacts with SIRP alpha and several other known CD47/SIRP alpha -blocking mAb. CD47 is broadly distributed on normal adult tissues, as well as ovarian tumors, and is especially abundant in some epithelia and the brain. IAP or integrin assocaited protein has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It further plays an important role in memory formation and synaptic plasticity in the hippocampus. It also acts as a receptor for SIRPA. Binding to SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Target Information
CD47, known as integrin-associated protein (IAP), is a glycosylated transmembrane glycoprotein with five domains, expressed widely across hematopoietic cells like T and B cells, monocytes, platelets, and erythrocytes, as well as non-hematopoietic cells. It interacts with integrins such as CD51/CD61 and CD41/CD61, and serves as a receptor for thrombospondin, mediating bi-directional signaling that affects neural synaptic activity and macrophage phagocytosis. CD47 acts as a ligand for CD172a (SIRP alpha), an inhibitory receptor on macrophages, preventing phagocytosis of CD47-positive cells. This interaction influences cell migration, B cell adhesion, T cell activation, and neuronal development, particularly in synapse-rich brain and retina regions. It also modulates chondrocyte responses to mechanical signals. T cell expression of CD47 can lead to activation or apoptosis in the presence of thrombospondin. Monoclonal antibody stimulation of CD47 has been shown to induce CD4+CD25- suppressive activity and increase Foxp3 expression. CD47's role in membrane transport, signal transduction, and its broad tissue distribution highlight its significance in various physiological processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: antigen identified by monoclonal antibody 1D8; Antigenic surface determinant protein OA3; CD47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; Leukocyte surface antigen CD47; Protein MER6; Rh-related antigen; sCD47; soluble CD 47; soluble CD47
Gene Aliases: CD47; IAP; MER6; OA3
UniProt ID: (Human) Q08722
Entrez Gene ID: (Human) 961
cell adhesion
integrin-mediated signaling pathway
opsonization
positive regulation of cell proliferation
response to bacterium
positive regulation of cell-cell adhesion
extracellular matrix organization
positive regulation of inflammatory response
positive regulation of phagocytosis
positive regulation of T cell activation
leukocyte migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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