Invitrogen

CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), APC-eFluor™ 780, eBioscience™

4 Published Figures

5 References

View all (68) CD49b (Integrin alpha 2) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

CD49b (Integrin alpha 2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD49b (Integrin alpha 2) Antibody (47-5971-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 PE-Cy7 (Product # 25-5941-82) and 0.125 µg of Anti-Mouse CD49b (Integrin alpha 2) APC-eFluor® 780. Cells in the lymphocyte gate were used for analysis.

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Product Details

47-5971-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 5 publications 5 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

DX5

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11218895

Product Specific Information

Description: The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer cells and a subset of T cells. DX5 reacts with all strains of mouse tested including the most commonly used strains, BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL and 129. Simultaneous staining of C57BL/6 spleen cells with anti-NK1.1 mAb (PK136) and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.

Applications Reported: This DX5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DX5 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse spleen cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 47-5971-82) in Flow Cytometry

Thu Oct 12 00:00:00 UTC 2023

Stage-specific action of Runx1 and GATA3 controls silencing of PU.1 expression in mouse pro-T cells.

The Journal of experimental medicine

Hosokawa H,Koizumi M,Masuhara K,Romero-Wolf M,Tanaka T,Nakayama T,Rothenberg EV

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 47-5971-82) in Flow Cytometry

Mon Aug 02 00:00:00 UTC 2021

Differential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase.

PloS one

Ender F,Wiese AV,Schmudde I,Sun J,Vollbrandt T,König P,Laumonnier Y,Köhl J

47-5971 was used in Flow cytometry/Cell sorting to demonstrate a complex regulation pattern of C5aR1 in the airways, lung tissue and lymph nodes of mice.

Thu Aug 17 00:00:00 UTC 2017

Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.

Nature communications

Krzywinska E,Kantari-Mimoun C,Kerdiles Y,Sobecki M,Isagawa T,Gotthardt D,Castells M,Haubold J,Millien C,Viel T,Tavitian B,Takeda N,Fandrey J,Vivier E,Sexl V,Stockmann C

47-5971 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which loss of HIF1α in natural killer cells inhibits tumour growth.

Fri Nov 17 00:00:00 UTC 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD49b (Integrin alpha 2) monoclonal antibody (Product # 47-5971-82) in Flow Cytometry

Thu Sep 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; Integrin alpha-2; Platelet membrane glycoprotein Ia; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLA2 receptor

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Gene Aliases: CD49B; DX5; Itga2

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UniProt ID: (Mouse) Q62469

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Entrez Gene ID: (Mouse) 16398

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Function(s)

integrin binding collagen binding protein complex binding collagen receptor activity laminin binding metal ion binding protein heterodimerization activity collagen binding involved in cell-matrix adhesion

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD49b (Integrin alpha 2) Monoclonal Antibody (DX5), APC-eFluor™ 780, eBioscience™

CD49b (Integrin alpha 2) Antibody (47-5971-82) in Flow

Product Details

47-5971-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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