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Invitrogen
CD49b (Integrin alpha 2) Monoclonal Antibody (eBioY418 (Y418)), eBioscience™
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FIGURE: 1 / 3
CD49b (Integrin alpha 2) Antibody (14-0498-95) in ICC/IF
Immunofluorescence analysis of CD49b/ITGA2 was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD49b/ITGA2 Mouse Monoclonal Antibody (Product # 14-0498-80) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse I... View More
Product Details
14-0498-95
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
eBioY418 (Y418)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
AB_2864901
Product Specific Information
Description: The eBioY418 (Y418) monoclonal antibody reacts with human CD49b, the 150-kDa, integrin alpha 2 subunit. The complex of CD49b non-covalently associated with integrin beta 1 (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by lymphocytes, activated T cells, monocytes and platelets.
Applications Reported: This eBioY418 (Y418) antibody has been reported for use in flow cytometric analysis. (Fluorochrome-conjugated eBioY418 is recommended for use in flow cytometry.).
Applications Tested: This eBioY418 (Y418) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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