Invitrogen

CD49c (Integrin alpha 3) Monoclonal Antibody (P1B5), APC, eBioscience™

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View all (29) CD49c (Integrin alpha 3) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

17-0494-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test
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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

P1B5

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10670221

Product Specific Information

Description: This P1B5 monoclonal antibody reacts with human CD49c (also known as Integrin alpha 3). Integrins constitute a family of cell surface receptors that mediate adhesion between cells or between a cell and the extracellular matrix. These proteins are heterodimers consisting of two distinct subunits, alpha and beta. Integrins are expressed on hematopoietic, non-hematopoietic, and cancer cells. Integrin-mediated adhesion is regulated by changes in ligand affinity (or activation), cell shape, and integrin clustering and diffusion. Integrin alpha 3 forms a complex with the common beta 1 subunit to form the alpha3beta1 integrin. Studies have demonstrated the involvement of this integrin in wound healing and epithelial migration.

The P1B5 monoclonal antibody has been reported to affect adhesion to type I and VI collagens, as well as fibronectin and laminin.

Applications Reported: This P1B5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This P1B5 antibody has been pre-titrated and tested by flow cytometric analysis of HeLa cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ITGA3 (INTEGRIN, ALPHA-3), also called CD49C, VLA3 or GAPB3, is a protein that in humans is encoded by the ITGA3 gene. ITGA3 is an integrin alpha subunit which is also a member of the family of cell surface adhesion molecules. ITGA3 is mapped to chromosome 17 and its exact cytogenetic location is 17q21.33. ITGA3 makes up half of the alpha3beta1 integrin duplex that plays a role in neural migration and corticogenesis together with beta-1 subunit. A functional link between DAB1 phosphorylation and ITGA3 signaling drives the timely detachment of migrating neurons from radial glial fibers. Expression of human ITGA3 increased the infectivity of virus for Chinese hamster ovary cells. ITGA3 also contains 13 potential N-glycosylation sites, 2 potential cleavage sites, and the 7 N-terminal repeating units characteristic of ITGAs. Recombinant ITGA3 is expressed as a 150-kD protein as the same size as the native protein by the western blot analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: alpha 3 subunit of VLA-3 receptor; Alpha3 integrin; antigen CD49C; antigen identified by monoclonal antibody J143; CD49 antigen-like family member C; CD49c; CD49c antigen; FLJ34631; FLJ34704; FRP-2; Galactoprotein B3; GAPB3; integrin alpha 3; Integrin alpha-3; Integrin alpha3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); testicular tissue protein Li 96; testicular tissue protein Li 98; very late activation protein 3 receptor, alpha-3 subunit; VLA-3 subunit alpha

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Gene Aliases: CD49C; FRP-2; GAP-B3; GAPB3; ILNEB; ITGA3; MSK18; VCA-2; VL3A; VLA3a

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UniProt ID: (Human) P26006

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Entrez Gene ID: (Human) 3675

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