Invitrogen

CD49d (Integrin alpha 4) Monoclonal Antibody (9F10), eBioscience™

CD49d (Integrin alpha 4) Antibody in Immunocytochemistry (ICC/IF)

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CD49d (Integrin alpha 4) Antibody (14-0499-82) in ICC/IF

Immunofluorescence analysis of CD49d (Integrin alpha 4) was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD49d (Integrin alpha 4) Monoclonal Antibody (9F10), eBioscience™ (Product # 14-0499-80) at ... View More

CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)

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Product Details

14-0499-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

Flow Cytometry (Flow)

0.25 µg/test

View 9 publications 9 publications

ELISA (ELISA)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 2 publications 2 publications

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

9F10

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_467292

Product Specific Information

Description: The 9F10 monoclonal antibody reacts with human CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin beta7 and binds to the Mucosal Addressin Cell-Adhesion Molecule-1 (MadCAM-1).

Applications Reported: The 9F10 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. 9F10 has also been reported in in vitro functional assays. (Please use Functional Grade purified 9F10, Product # 16-0499, in functional assays).

Applications Tested: The 9F10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CD8⁺ lymphocytes do not impact SIV reservoir establishment under ART.

Nature microbiology

Statzu M,Jin W,Fray EJ,Wong AKH,Kumar MR,Ferrer E,Docken SS,Pinkevych M,McBrien JB,Fennessey CM,Keele BF,Liang S,Harper JL,Mutascio S,Franchitti L,Wang H,Cicetti D,Bosinger SE,Carnathan DG,Vanderford TH,Margolis DM,Garcia-Martinez JV,Chahroudi A,Paiardini M,Siliciano J,Davenport MP,Kulpa DA,Siliciano RS,Silvestri G

14-0499-82 was used in Flow cytometry/Cell sorting to indicate that during early SIV infection, the viral reservoir that persists under ART is established largely independent of CTL control.

Wed Feb 01 00:00:00 UTC 2023

Mesenchymal stem cell aggregation mediated by integrin α4/VCAM-1 after intrathecal transplantation in MCAO rats.

Stem cell research & therapy

Ran Y,Dong Y,Li Y,Xie J,Zeng S,Liang C,Dai W,Tang W,Wu Y,Yu S

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 14-0499-82) in Flow Cytometry

Sat Oct 22 00:00:00 UTC 2022

Identification of potential chemical compounds enhancing generation of enucleated cells from immortalized human erythroid cell lines.

Communications biology

Soboleva S,Kurita R,Ek F,Åkerstrand H,Silvério-Alves R,Olsson R,Nakamura Y,Miharada K

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 14-0499-82) in Flow Cytometry

Thu Jun 03 00:00:00 UTC 2021

A Prime/Boost Vaccine Regimen Alters the Rectal Microbiome and Impacts Immune Responses and Viremia Control Post-Simian Immunodeficiency Virus Infection in Male and Female Rhesus Macaques.

Journal of virology

Musich T,Thovarai V,Venzon DJ,Mohanram V,Tuero I,Miller-Novak LK,Helmold Hait S,Rahman MA,Hunegnaw R,Huiting E,Yuan W,O'hUigin C,Hoang T,Sui Y,LaBranche C,Montefiori D,Bear J,Rosati M,Bissa M,Berzofsky JA,Pavlakis GN,Felber BK,Franchini G,Robert-Guroff M

14-0499-82 was used in Flow Cytometry to highlight the critical importance of considering effects of sex and the microbiome in vaccine design and evaluation.

Mon Nov 23 00:00:00 UTC 2020

Enhanced control of Mycobacterium tuberculosis extrapulmonary dissemination in mice by an arabinomannan-protein conjugate vaccine.

PLoS pathogens

Prados-Rosales R,Carreño L,Cheng T,Blanc C,Weinrick B,Malek A,Lowary TL,Baena A,Joe M,Bai Y,Kalscheuer R,Batista-Gonzalez A,Saavedra NA,Sampedro L,Tomás J,Anguita J,Hung SC,Tripathi A,Xu J,Glatman-Freedman A,Jacobs WR,Chan J,Porcelli SA,Achkar JM,Casadevall A

14-0499 was used in Flow cytometry/Cell sorting to develop capsular polysaccharide conjugates for enhanced control of Mycobacterium tuberculosis extrapulmonary dissemination in mice.

Wed Mar 01 00:00:00 UTC 2017

The APPEESFRS Peptide, Restricted by the HLA-B*35:01 Molecule, and the APPEESFRF Variant Derived from an Autologous HIV-1 Strain Induces Polyfunctional Responses in CD8+ T Cells.

BioResearch open access

Acevedo-Sáenz L,Carmona-Pérez L,Velilla-Hernández PA,Delgado JC,Rugeles L MT

14-0499 was used in Flow cytometry/Cell sorting to determine the effector profile and maturation phenotype of CD8+ T-cells targeting the consensus APPEESFRS (AS9) epitope and its variant APPEESFRF (AF9).

Fri Aug 28 00:00:00 UTC 2015

LDL cholesterol modulates human CD34+ HSPCs through effects on proliferation and the IL-17 G-CSF axis.

PloS one

Cimato TR,Palka BA,Lang JK,Young RF

14-0499 was used in Flow cytometry/Cell sorting to determine how cholesterol levels affect haematopoietic stem/progenitor cell quantity in humans.

Sun Jun 15 00:00:00 UTC 2014

Protection against H5N1 highly pathogenic avian and pandemic (H1N1) 2009 influenza virus infection in cynomolgus monkeys by an inactivated H5N1 whole particle vaccine.

PloS one

Nakayama M,Shichinohe S,Itoh Y,Ishigaki H,Kitano M,Arikata M,Pham VL,Ishida H,Kitagawa N,Okamatsu M,Sakoda Y,Ichikawa T,Tsuchiya H,Nakamura S,Le QM,Ito M,Kawaoka Y,Kida H,Ogasawara K

14-0499 was used in Flow cytometry/Cell sorting to examine the efficacy of the H5N1 vaccine candidate Vac-3 against two clades of H5N1 HPAIV strains in cynomolgus macaques.

Tue Mar 03 00:00:00 UTC 2015

Incomplete normalization of regulatory t-cell frequency in the gut mucosa of Colombian HIV-infected patients receiving long-term antiretroviral treatment.

PloS one

Rueda CM,Velilla PA,Chougnet CA,Rugeles MT

14-0499 was used in Functional assays to assess the frequency and phenotype of regulatory T-cells within CMV and HIV patients on different treatment regimes.

Tue Sep 23 00:00:00 UTC 2014

Rv2468c, a novel Mycobacterium tuberculosis protein that costimulates human CD4+ T cells through VLA-5.

Journal of leukocyte biology

Li Q,Ding X,Thomas JJ,Harding CV,Pecora ND,Ziady AG,Shank S,Boom WH,Lancioni CL,Rojas RE

14-0499 was used in Blocking experiments to report that a previously uncharacterised Mycobacterium tuberculosis protein can directly regulate human CD4+ T cell activation by delivering costimulatory signals.

Wed Feb 01 00:00:00 UTC 2012

Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02.

PloS one

Arikata M,Itoh Y,Okamatsu M,Maeda T,Shiina T,Tanaka K,Suzuki S,Nakayama M,Sakoda Y,Ishigaki H,Takada A,Ishida H,Soda K,Pham VL,Tsuchiya H,Nakamura S,Torii R,Shimizu T,Inoko H,Ohkubo I,Kida H,Ogasawara K

14-0499 was used in Functional assays to examine the effectiveness of a vaccine derived from a virus library for pandemic virus infection in immunologically naïve macaques.

Wed Sep 19 00:00:00 UTC 2012

Characterization of interleukin-17-producing regulatory T cells in inflamed intestinal mucosa from patients with inflammatory bowel diseases.

Gastroenterology

Hovhannisyan Z,Treatman J,Littman DR,Mayer L

14-0499 was used in Flow cytometry/Cell sorting to investigate how Th17 and Tregs interact under chronic inflammatory conditions.

Tue Mar 01 00:00:00 UTC 2011

Expression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung.

Respiratory research

Kawamata N,Xu B,Nishijima H,Aoyama K,Kusumoto M,Takeuchi T,Tei C,Michie SA,Matsuyama T

14-0499 was used in Immunohistochemistry to identify which adhesion molecules recruit specific subsets of lymphocytes into human bronchus-associated lymphoid tissue.

Thu Oct 22 00:00:00 UTC 2009

Interleukin-15 increases effector memory CD8+ t cells and NK Cells in simian immunodeficiency virus-infected macaques.

Journal of virology

Mueller YM,Petrovas C,Bojczuk PM,Dimitriou ID,Beer B,Silvera P,Villinger F,Cairns JS,Gracely EJ,Lewis MG,Katsikis PD

14-0499 was used in an ELISpot assay to investigate the in vivo effect of IL-15 treatment in nine SIVmac251-infected cynomolgus macaques.

Fri Apr 01 00:00:00 UTC 2005

Interleukin-15 increases effector memory CD8+ t cells and NK Cells in simian immunodeficiency virus-infected macaques.

Journal of virology

Mueller YM,Petrovas C,Bojczuk PM,Dimitriou ID,Beer B,Silvera P,Villinger F,Cairns JS,Gracely EJ,Lewis MG,Katsikis PD

14-0499 was used in an ELISpot assay to investigate the in vivo effect of IL-15 treatment in nine SIVmac251-infected cynomolgus macaques.

Fri Apr 01 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: 269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; ITGA-IV; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha

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Gene Aliases: CD49D; IA4; ITGA4

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UniProt ID: (Human) P13612

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Entrez Gene ID: (Human) 3676

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Function(s)

fibronectin binding protein binding coreceptor activity C-X3-C chemokine binding metal ion binding cell adhesion molecule binding protein antigen binding

Process(es)

cell-matrix adhesion involved in ameboidal cell migration leukocyte cell-cell adhesion cell-matrix adhesion integrin-mediated signaling pathway B cell differentiation extracellular matrix organization heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading endodermal cell differentiation receptor clustering regulation of immune response leukocyte migration leukocyte tethering or rolling diapedesis cellular response to cytokine stimulus negative regulation of protein homodimerization activity import into cell positive regulation of leukocyte tethering or rolling clathrin-mediated extracellular exosome endocytosis positive regulation of T cell migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD49d (Integrin alpha 4) Monoclonal Antibody (9F10), eBioscience™

CD49d (Integrin alpha 4) Antibody (14-0499-82) in ICC/IF

Product Details

14-0499-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (Functional)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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