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Invitrogen
CD49d (Integrin alpha 4) Monoclonal Antibody (MRa4-1), APC, eBioscience™
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FIGURE: 1 / 1
CD49d (Integrin alpha 4) Antibody (17-1490-82) in Flow
Staining of rat splenocytes with Anti-Rat CD45R (B220) FITC (Product # 11-0460) and 0.25 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724) (left) or 0.25 µg of Anti-Rat CD49d (Integrin alpha 4) APC (right). Cells in the lymphocyte gate were used for analysis.
Product Details
17-1490-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MRa4-1
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573164
Product Specific Information
Description: The MRa4-1 monoclonal antibody reacts with CD49d, which is also known as the integrin alpha 4 subunit. This 150-kDa cell surface protein is expressed by thymocytes, peripheral lymphocytes, monocytes, and eosinophils in complex with either integrin beta1 or integrin beta7. Outside of the immune system, CD49d is also expressed on mast cells, neural crest cells, retinal cells, and dorsal root ganglion neurons. Integrins are responsible for lymphocyte homing to Peyer's patches and mesenteric lymph nodes, as well as neural development and regeneration. Several ligands have been reported for the integrin a4b1 complex, including VCAM-1 (CD106) and fibronectin.
The MRa1-4 antibody has been reported to inhibit fibronectin binding and lymphocyte migration.
Applications Reported: This MRa4-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MRa4-1 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: integrin alpha-4; ITGA-IV
Entrez Gene ID: (Rat) 311144
blood vessel remodeling
positive regulation of leukocyte migration
cell-matrix adhesion involved in ameboidal cell migration
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
heart development
cell migration
heterotypic cell-cell adhesion
substrate adhesion-dependent cell spreading
endodermal cell differentiation
receptor clustering
leukocyte tethering or rolling
diapedesis
face development
chorio-allantoic fusion
cellular response to cytokine stimulus
T cell migration
negative regulation of protein homodimerization activity
import into cell
positive regulation of leukocyte cell-cell adhesion
positive regulation of leukocyte tethering or rolling
positive regulation of T cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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