Invitrogen

CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), Biotin, eBioscience™

11 Published Figures

8 References

View all (55) CD49d (Integrin alpha 4) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (11)

CD49d (Integrin alpha 4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 12

CD49d (Integrin alpha 4) Antibody (13-0492-82) in Flow

Surface staining of mouse splenocytes with Anti-Mouse CD49d (Integrin alpha 4) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

13-0492-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

R1-2

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_466474

Product Specific Information

Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1).

Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Low immunogenicity of malaria pre-erythrocytic stages can be overcome by vaccination.

EMBO molecular medicine

Müller K,Gibbins MP,Roberts M,Reyes-Sandoval A,Hill AVS,Draper SJ,Matuschewski K,Silvie O,Hafalla JCR

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 13-0492-82) in Flow Cytometry

Fri Apr 09 00:00:00 UTC 2021

A Macrophage Colony-Stimulating-Factor-Producing γδ T Cell Subset Prevents Malarial Parasitemic Recurrence.

Immunity

Mamedov MR,Scholzen A,Nair RV,Cumnock K,Kenkel JA,Oliveira JHM,Trujillo DL,Saligrama N,Zhang Y,Rubelt F,Schneider DS,Chien YH,Sauerwein RW,Davis MM

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 13-0492-82) in Flow Cytometry

Tue Feb 20 00:00:00 UTC 2018

Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis.

Frontiers in immunology

Kobori T,Hamasaki S,Kitaura A,Yamazaki Y,Nishinaka T,Niwa A,Nakao S,Wake H,Mori S,Yoshino T,Nishibori M,Takahashi H

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 13-0492-82) in Flow Cytometry

Wed Mar 27 00:00:00 UTC 2019

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 13-0492-82) in Flow Cytometry

Tue Oct 30 00:00:00 UTC 2018

Intestinal helminth infection induces highly functional resident memory CD4⁺ T cells in mice.

European journal of immunology

Steinfelder S,Rausch S,Michael D,Kühl AA,Hartmann S

13-0492 was used in Flow cytometry/Cell sorting to show that nonlymphoid compartments can serve as reservoirs for Th2 memory cells, and furthermore that innate effector function of Th2 memory cells is restricted to CD4⁺ memory T cells residing in the peritoneal cavity.

Wed Feb 01 00:00:00 UTC 2017

The impact of ranitidine on monocyte responses in the context of solid tumors.

Oncotarget

Vila-Leahey A,Rogers D,Marshall JS

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 13-0492-82) in Flow Cytometry

Tue Mar 08 00:00:00 UTC 2016

Exploring the Functional Complementation between Grp94 and Hsp90.

PloS one

Maharaj KA,Que NL,Hong F,Huck JD,Gill SK,Wu S,Li Z,Gewirth DT

13-0492 was used in Flow cytometry/Cell sorting to identify the interface between the Middle and C-terminal domains as an important structural unit within the Hsp90 family.

Tue Jun 27 00:00:00 UTC 2017

Interleukin-7 and anti-programmed cell death 1 antibody have differing effects to reverse sepsis-induced immunosuppression.

Shock (Augusta, Ga.)

Shindo Y,Unsinger J,Burnham CA,Green JM,Hotchkiss RS

Published figure using CD49d (Integrin alpha 4) monoclonal antibody (Product # 13-0492-82) in Flow Cytometry

Wed Apr 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; ITGA-IV; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha

View more View less

Gene Aliases: CD49D; Itga4; Itga4B

View more View less

UniProt ID: (Mouse) Q00651

View more View less

Entrez Gene ID: (Mouse) 16401

View more View less

Function(s)

fibronectin binding metal ion binding cell adhesion molecule binding protein antigen binding

Process(es)

blood vessel remodeling positive regulation of leukocyte migration cell-matrix adhesion involved in ameboidal cell migration cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion integrin-mediated signaling pathway heart development single organismal cell-cell adhesion cell migration heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading receptor clustering leukocyte tethering or rolling diapedesis face development mesenchyme development chorio-allantoic fusion visceral serous pericardium development T cell migration negative regulation of protein homodimerization activity import into cell positive regulation of leukocyte cell-cell adhesion positive regulation of leukocyte tethering or rolling positive regulation of T cell migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), Biotin, eBioscience™

CD49d (Integrin alpha 4) Antibody (13-0492-82) in Flow

Product Details

13-0492-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!