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Invitrogen
CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), Functional Grade, eBioscience™
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FIGURE: 1 / 12
CD49d (Integrin alpha 4) Antibody (16-0492-025) in Flow
Mouse splenocytes were stained with 0.25 µg of Rat IgG2b kappa Isotype Control (Product # 14-4031-85) (blue histogram) or 0.25 µg of CD49d Monoclonal Antibody, Functional Grade (purple histogram) followed by IgG2b Monoclonal Antibody, PE (Product # 12-4815-82). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
16-0492-025
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
R1-2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
AB_2865747
Product Specific Information
Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1).
Applications Reported: The R1-2 antibody has been reported for use in flow cytometric analysis. R1-2 has also been reported in in vitro functional assays.
Applications Tested: The R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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