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Invitrogen
CD49e (Integrin alpha 5) Monoclonal Antibody (eBioHMa5-1 (HMa5-1)), eBioscience™
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-Flow-20170216130641.jpg?time=20250104 "CD49e (Integrin alpha 5) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 1
CD49e (Integrin alpha 5) Antibody (14-0493-81) in Flow
Staining of C57Bl/6 bone marrow cells with 0.5 µg of Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (left) or 0.5 µg of Anti-Mouse/Rat CD49e (Integrin alpha 5) Purified (right) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111). Total viable cells were used for analysis.
-Flow-20170216130641.jpg?time=20250104)
-Flow-20170216130641.jpg?time=20250104?time=20250104 "CD49e (Integrin alpha 5) Antibody (14-0493-81) in Flow")
Product Details
14-0493-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
eBioHMa5-1 (HMa5-1)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_763507
Product Specific Information
Description: The eBioHMalpha5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin alpha5 or VLA-5A. CD49e is a member of the integrin alpha chain family, and binds with CD29 (integrin beta1) to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The eBioHMalpha5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates. Furthermore, HMalpha5-1 injected into mice subcutaneously, along with anti-CD49d and anti-CD61, is able to inhibit passive cutaneous anaphylaxis.
Applications Reported: This eBioHMa5-1 (HMa5-1) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. (Please use Functional Grade purified eBioHMa5-1 (HMa5-1), Product # 16-0493, in functional assays).
Applications Tested: This eBioHMa5-1 (HMa5-1) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD49 antigen-like family member E; CD49e; fibronectin receptor alpha polypeptide; Fibronectin receptor subunit alpha; integrin alpha 5 (fibronectin receptor alpha); Integrin alpha-5; Integrin alpha-F; VLA-5
Gene Aliases: Cd49e; Fnra; Itga5; Itga5_retired; VLA5
UniProt ID: (Mouse) P11688
Entrez Gene ID: (Mouse) 16402, (Rat) 315346
cell-substrate junction assembly
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
integrin-mediated signaling pathway
memory
positive regulation of cell-substrate adhesion
positive regulation of cell migration
cell-substrate adhesion
cell-cell adhesion mediated by integrin
heterotypic cell-cell adhesion
endodermal cell differentiation
positive regulation of peptidyl-tyrosine phosphorylation
negative regulation of anoikis
wound healing, spreading of epidermal cells
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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