Invitrogen

CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), eBioscience™

1 Published Figure
5 References
View all (12) CD51/CD61 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

14-0519-82

Applications
Tested Dilution
Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent
-

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test
View 1 publication 1 publication

Neutralization (Neu)

-
View 1 publication 1 publication

Inhibition Assays (IA)

-
View 1 publication 1 publication

In vitro Assay (IV)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

23C6

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467299

Product Specific Information

Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.

Applications Reported: The 23C6 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. 23C6 has also been reported in blocking of some adhesive processes. (Please use Functional Grade purified 23C6 in functional assays).

Applications Tested: The 23C6 antibody has been tested by flow cytometric analysis of human melanoma cell line and peripheral blood. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex. This complex binds vitronection at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: antigen CD61; antigen identified by monoclonal antibody L230; CD51; CD61; GPIIIa; integrin alpha V; integrin alpha-5 beta-3; Integrin alpha-V; integrin alphaVbeta3; integrin beta 3; Integrin beta-3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); Platelet membrane glycoprotein IIIa; Vitronectin receptor; Vitronectin receptor subunit alpha

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Gene Aliases: BDPLT16; BDPLT2; CD51; CD61; GP3A; GPIIIa; GT; ITGAV; ITGB3; MSK8; VNRA; VTNR

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UniProt ID: (Human) P06756, (Human) P05106

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Entrez Gene ID: (Human) 3685, (Human) 3690

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Function(s)
virus receptor activity opsonin binding fibronectin binding protease binding protein kinase C binding voltage-gated calcium channel activity protein binding coreceptor activity fibroblast growth factor binding C-X3-C chemokine binding insulin-like growth factor I binding neuregulin binding metal ion binding transforming growth factor beta binding extracellular matrix binding extracellular matrix protein binding protein disulfide isomerase activity platelet-derived growth factor receptor binding enzyme binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding
Process(es)
positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation platelet degranulation cell-substrate junction assembly cell adhesion cell-matrix adhesion integrin-mediated signaling pathway blood coagulation positive regulation of endothelial cell migration negative regulation of macrophage derived foam cell differentiation negative regulation of lipid storage smooth muscle cell migration cell growth cell migration platelet activation extracellular matrix organization positive regulation of vascular endothelial growth factor receptor signaling pathway cell-substrate adhesion activation of protein kinase activity negative regulation of lipid transport heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading tube development apolipoprotein A-I-mediated signaling pathway wound healing regulation of bone resorption negative regulation of low-density lipoprotein particle receptor biosynthetic process viral entry into host cell vascular endothelial growth factor receptor signaling pathway mesodermal cell differentiation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of lipoprotein metabolic process leukocyte migration negative chemotaxis angiogenesis involved in wound healing platelet aggregation angiogenesis antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent positive regulation of cell proliferation positive regulation of cell migration positive regulation of osteoblast proliferation endodermal cell differentiation apoptotic cell clearance positive regulation of cell adhesion regulation of phagocytosis entry of symbiont into host cell by promotion of host phagocytosis ERK1 and ERK2 cascade calcium ion transmembrane transport extrinsic apoptotic signaling pathway in absence of ligand regulation of apoptotic cell clearance negative regulation of entry of bacterium into host cell negative regulation of extrinsic apoptotic signaling pathway
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