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Invitrogen
CD51 (Integrin alpha V) Monoclonal Antibody (RMV-7), eBioscience™
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FIGURE: 1 / 8
CD51 (Integrin alpha V) Antibody (14-0512-82) in Flow
Staining of C57Bl/6 bone marrow cells with 1 µg of Rat IgG1 Isotype Control Purified (Product # 14-4301) (open histogram) or 1 µg of Anti-Mouse CD51 (Integrin alpha V) Purified (filled histogram) followed by Anti-Rat IgG PE (Product # 12-4822). Cells in the large scatter population were used for analysis.
Product Details
14-0512-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
RMV-7
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_467296
Product Specific Information
Description: The RMV-7 monoclonal antibody reacts with the mouse CD51 molecule, the integrin alpha v chain. This approximately 120 kDa surface molecule non-covalently associates with the beta subunits of the integrin family including beta3 (CD61), beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by platelets, T cells and granulocytes and mediate adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.
Applications Reported: The RMV-7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and functional assays. Soluble RMV-7 blocks adhesion to fibronectin and vitronectin and immobilized RMV-7 induces activation. (Please use Functional Grade purified RMV-7, Product # 16-0512, in functional assays).
Applications Tested: The RMV-7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8, 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands, the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha v integrin; CD51; CD61; GP3A; GPIIIa; Integrin alpha-V; ITGB3; vitronectin receptor alpha polypeptide (VNRA); Vitronectin receptor subunit alpha; VNR
Gene Aliases: 1110004F14Rik; 2610028E01Rik; CD51; D430040G12Rik; Itgav
UniProt ID: (Mouse) P43406
Entrez Gene ID: (Mouse) 16410
opsonin binding
fibronectin binding
protease binding
protein kinase C binding
receptor binding
voltage-gated calcium channel activity
insulin-like growth factor I binding
peptide binding
metal ion binding
protein heterodimerization activity
extracellular matrix binding
extracellular matrix protein binding
angiogenesis
blood vessel development
cellular calcium ion homeostasis
inflammatory response
cell adhesion
cell-matrix adhesion
positive regulation of cytosolic calcium ion concentration
integrin-mediated signaling pathway
multicellular organism development
positive regulation of cell proliferation
fertilization
negative regulation of macrophage derived foam cell differentiation
negative regulation of lipid storage
cell growth
cell migration
cell differentiation
positive regulation of cell migration
cell-substrate adhesion
negative regulation of lipid transport
detection of molecule of bacterial origin
positive regulation of osteoblast proliferation
heterotypic cell-cell adhesion
substrate adhesion-dependent cell spreading
regulation of ion transmembrane transport
gonad morphogenesis
endodermal cell differentiation
apolipoprotein A-I-mediated signaling pathway
T cell activation
apoptotic cell clearance
positive regulation of MAPK cascade
endothelial cell migration
regulation of bone resorption
negative regulation of low-density lipoprotein particle receptor biosynthetic process
positive regulation of cell adhesion
viral entry into host cell
focal adhesion assembly
negative regulation of lipoprotein metabolic process
regulation of phagocytosis
negative chemotaxis
release of sequestered calcium ion into cytosol
trophoblast giant cell differentiation
ERK1 and ERK2 cascade
calcium ion transmembrane transport
response to nitric oxide
extrinsic apoptotic signaling pathway in absence of ligand
regulation of apoptotic cell clearance
negative regulation of entry of bacterium into host cell
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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