Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (HA58), NovaFluor™ Red 685, eBioscience™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

H035T03R02-A

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.4 µg)/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

HA58

Conjugate

NovaFluor™ Red 685 NovaFluor™ Red 685 NovaFluor™ Red 685

Excitation/Emission Max

637/685 nm View spectra spectra

Form

Liquid

Concentration

0.4 µg/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

RRID

AB_3098449 (AB_2921112)

Product Specific Information

Description: The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response.

Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .

Applications Reported: The HA58 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The HA58 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 5 µL (0.4 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.

NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 637 nm; Emission: 685 nm; Laser: 633-640 nm (Red) Laser

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Bioinformatics

Protein Aliases: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells

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Gene Aliases: BB2; CD54; ICAM1; P3.58

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UniProt ID: (Human) P05362

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Entrez Gene ID: (Human) 3383

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Function(s)
virus receptor activity receptor activity transmembrane signaling receptor activity integrin binding protein binding
Process(es)
ovarian follicle development regulation of leukocyte mediated cytotoxicity response to amphetamine T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell acute inflammatory response to antigenic stimulus T cell antigen processing and presentation positive regulation of cellular extravasation cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell aging sensory perception of sound regulation of cell shape response to ionizing radiation response to sulfur dioxide response to organic cyclic compound membrane to membrane docking regulation of cell adhesion extracellular matrix organization positive regulation of actin filament polymerization cellular response to nutrient levels cell adhesion mediated by integrin response to gonadotropin response to drug response to amino acid positive regulation of GTPase activity adhesion of symbiont to host positive regulation of nitric oxide biosynthetic process response to ethanol positive regulation of vasoconstriction response to copper ion viral entry into host cell receptor-mediated virion attachment to host cell positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response leukocyte migration positive regulation of NF-kappaB transcription factor activity negative regulation of calcium ion transport interferon-gamma-mediated signaling pathway establishment of endothelial barrier positive regulation of ERK1 and ERK2 cascade cellular response to lipopolysaccharide cellular response to alkaloid cellular response to glucose stimulus cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to hypoxia establishment of Sertoli cell barrier regulation of ruffle assembly negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of endothelial cell apoptotic process
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