Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), PE, eBioscience™

1 Published Figure

6 References

View all (171) ICAM-1 (CD54) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD54 (ICAM-1) Antibody (12-0542-82) in Flow

Staining of C57BL/6 splenocytes with 0.06 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (open histogram) or 0.06 µg of Anti-Mouse CD54 (ICAM-1) PE (filled histogram). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

12-0542-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioKAT-1 (KAT-1, KAT1)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_529540

Product Specific Information

Description: The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), which is a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, and expression increases in response to inflammatory cytokines. ICAM-1 binds to LFA-1 and this interaction is required for the transendothelial migration of T cells. ICAM-1-deficient mice are viable, however the migration of leukocytes to sites of inflammation is reduced leading to impaired immune and inflammatory responses. Based on the regulated expression of ICAM-1, it has been suggested that ICAM-1 may increase leukocyte extravasation at sites of inflammation, whereas the constitutively high expression of ICAM-2 mediates leukocyte traffic into non-inflamed tissue. The eBioKAT-1 monoclonal antibody recognizes a different epitope than the YN1/1.7.4 monoclonal antibody.

Applications Reported: This eBioKAT-1 (KAT-1, KAT1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioKAT-1 (KAT-1, KAT1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

JMJD3 acts in tandem with KLF4 to facilitate reprogramming to pluripotency.

Nature communications

Huang Y,Zhang H,Wang L,Tang C,Qin X,Wu X,Pan M,Tang Y,Yang Z,Babarinde IA,Lin R,Ji G,Lai Y,Xu X,Su J,Wen X,Satoh T,Ahmed T,Malik V,Ward C,Volpe G,Guo L,Chen J,Sun L,Li Y,Huang X,Bao X,Gao F,Liu B,Zheng H,Jauch R,Lai L,Pan G,Chen J,Testa G,Akira S,Hu J,Pei D,Hutchins AP,Esteban MA,Qin B

12-0542 was used in Flow cytometry/Cell sorting to establish a multifaceted role for JMJD3, placing it as a key partner of KLF4 and a scaffold that assists chromatin interactions and activates gene transcription.

Thu Oct 08 00:00:00 UTC 2020

Induced pluripotent stem cell-derived mesenchymal stem cells activate quiescent T cells and elevate regulatory T cell response via NF-κB in allergic rhinitis patients.

Stem cell research & therapy

Fan XL,Zeng QX,Li X,Li CL,Xu ZB,Deng XQ,Shi J,Chen D,Zheng SG,Fu QL

12-0542 was used in Flow cytometry/Cell sorting to identify the immunomodulatory role of induced pluripotent stem cell-derived mesenchymal stem cells on resting peripheral blood mononuclear cells from allergic rhinitis patients.

Tue Jun 19 00:00:00 UTC 2018

Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming.

Cell reports

Simula L,Pacella I,Colamatteo A,Procaccini C,Cancila V,Bordi M,Tregnago C,Corrado M,Pigazzi M,Barnaba V,Tripodo C,Matarese G,Piconese S,Campello S

12-0542 was used in Flow cytometry/Cell sorting to describe the crucial role of the mitochondrial protein Drp1 in T cell development and regulation of lymphocyte immune-surveillance.

Tue Dec 11 00:00:00 UTC 2018

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

12-0542 was used in Flow cytometry/Cell sorting to determine that SENP1-mediated SUMOylation drives graft arteriosclerosis by regulating the synergistic effect of GATA2 and NF-κB.

Thu Jun 01 00:00:00 UTC 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

12-0542 was used in Flow cytometry/Cell sorting to determine that SENP1-mediated SUMOylation drives graft arteriosclerosis by regulating the synergistic effect of GATA2 and NF-κB.

Thu Jun 01 00:00:00 UTC 2017

P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.

Cell research

Wang H,Hong LJ,Huang JY,Jiang Q,Tao RR,Tan C,Lu NN,Wang CK,Ahmed MM,Lu YM,Liu ZR,Shi WX,Lai EY,Wilcox CS,Han F

12-0542 was used in Flow cytometry/Cell sorting to demonstrate the role of the P2RX7 pathway in linking neurovascular inflammation to brain damage in vivo.

Mon Jun 01 00:00:00 UTC 2015

Expression of intercellular adhesion molecule 1 by hepatocellular carcinoma stem cells and circulating tumor cells.

Gastroenterology

Liu S,Li N,Yu X,Xiao X,Cheng K,Hu J,Wang J,Zhang D,Cheng S,Liu S

12-0542 was used in Flow cytometry/Cell sorting to demonstrate that the stem cell factor Nanog regulates expression of ICAM-1 in hepatocellular carcinoma cells.

Wed May 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

View more View less

Gene Aliases: CD54; Icam-1; Icam1; Ly-47; MALA-2

View more View less

UniProt ID: (Mouse) P13597

View more View less

Entrez Gene ID: (Mouse) 15894

View more View less

Function(s)

integrin binding protein complex binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), PE, eBioscience™

CD54 (ICAM-1) Antibody (12-0542-82) in Flow

Product Details

12-0542-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!