Invitrogen

CD56 Monoclonal Antibody (123C3)

CD56 Antibody in Immunocytochemistry (ICC/IF)

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CD56 Antibody (MA1-35249) in ICC/IF

Immunofluorescence analysis of CD56 was performed using 70% confluent log phase IMR-32. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD56 Monoclonal Antibody (Product # MA1-35249) at 1:20 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:2000), for 4... View More

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CD56 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-CD56 Monoclonal Antibody (123C3) (Product # MA1-35249) shows increased expression of CD56 in IMR-32 cell line when compared to Hep G2 cell line. {RE}

Product Details

MA1-35249

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

1:50

Immunocytochemistry (ICC/IF)

1:5-1:20

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

123C3

Immunogen

Proprietary Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1073116

Product Specific Information

The antibody recognizes a transmembrane glycoprotein of 140 and 180 kD which has been identified as NCAM (Neural Cell Adhesion Module). At the international Workshop on SCLC antibodies 123C3 has been categorized as cluster 1 antibody. All cells in small cell carcinomas and carcinoids of the lung are strongly positive for 123C3. A minority of cases of other major types of lung carcinoma are sometimes positive as well: however this positivity is generally weak and focal. Adenoid cystic carcinomas of bronchial glands are strongly positive. Neuroblastoma's and Wilms tumors are usually also staining strongly positive. In non-small lung cell carcinomas, 123C3 staining has been associated with more advanced stage and a decreased survival after surgery. Furthermore, this antibody can be used to support diagnosis of lymphoma or to detect residual disease for cases of CD56 positive T/NK -cell lymphoma in which the neoplastic lymphoid cells are small and show minimal atypia, especially in small biopsies.

Target Information

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Usefulness of the octreotide test in Japanese patients for predicting the presence/absence of somatostatin receptor 2 expression in insulinomas.

Endocrine journal

Nakamura A,Mitsuhashi T,Takano Y,Miyoshi H,Kameda H,Nomoto H,Nagai S,Hatanaka Y,Shimizu C,Terauchi Y,Atsumi T

MA135249 was used in immunohistochemistry - paraffin section to assess the relationship between the results of the octreotide test and somatostatin receptor 2 expression in insulinoma patients to predict insulinomas in Japanese patients

Fri Dec 23 00:00:00 UTC 2016

Variable Expression of Neural Cell Adhesion Molecule Isoforms in Renal Tissue: Possible Role in Incipient Renal Fibrosis.

PloS one

Marković-Lipkovski J,Životić M,Müller CA,Tampe B,Ćirović S,Vještica J,Tomanović N,Zeisberg M,Müller GA

MA1-35249 was used in immunohistochemistry - paraffin section to learn the possible role of incipient renal fibrosis by variable expression of neural cell adhesion molecule isoforms in renal tissue

Wed May 18 00:00:00 UTC 2016

Role of cd56 and e-cadherin expression in the differential diagnosis of papillary thyroid carcinoma and suspected follicular-patterned lesions of the thyroid: the prognostic importance of e-cadherin.

International journal of clinical and experimental pathology

Ceyran AB,Şenol S,Şimşek BÇ,Sağıroğlu J,Aydın A

MA1-35249 was used in immunohistochemistry - paraffin section to discuss how to distinguish follicular variant of PTC from other follicular-patterned lesions of the thyroid.

Thu Mar 24 00:00:00 UTC 2016

CD30⁺ extranodal natural killer/T-cell lymphoma mimicking phlegmonous myositis: A case report.

Oncology letters

Yang YJ,Li YX,Liu YB,Yang M,Liu K

MA1-35249 was used in immunohistochemistry (paraffin) to report on a case of CD30-positive extranodal natural killer/T-cell lymphoma.

Thu May 01 00:00:00 UTC 2014

A Case of Distal Epithelioid Sarcoma of the Thumb Expressing Podoplanin, TLE1 and Ca 125.

Case reports in pathology

Karagkounis G,Argyrakos T,Charkiolakis G,Castana O,Rontogianni D

MA1-35249 was used in immunohistochemistry to characterize the case of a distal epithelioid sarcoma arising in the thumb of a 14-year-old girl

Wed May 22 00:00:00 UTC 2013

Serous effusion cytology of extranodal natural killer/T-cell lymphoma.

Cytopathology : official journal of the British Society for Clinical Cytology

Su XY,Huang J,Jiang Y,Tang Y,Li GD,Liu WP

MA1-35249 was used in flow cytometry to characterize extranodal natural killer/T-cell lymphoma, nasal type using patient samples.

Sun Apr 01 00:00:00 UTC 2012

Subcutaneous panniculitis-like T-cell lymphoma: a clinical and pathologic study of 14 korean patients.

Annals of dermatology

Lee DW,Yang JH,Lee SM,Won CH,Chang S,Lee MW,Choi JH,Moon KC

MA1-35249 was used in immunohistochemistry to study subcutaneous panniculitis-like T-cell lymphomas in Korean patients

Mon Aug 01 00:00:00 UTC 2011

Extraosseous (extramedullary) plasmacytomas: a clinicopathologic and immunophenotypic study of 32 Chinese cases.

Diagnostic pathology

Zuo Z,Tang Y,Bi CF,Zhang WY,Zhao S,Wang XQ,Yang QP,Zou LQ,Liu WP

MA1-35249 was used in immunohistochemistry - paraffin section to determine the clinicopathologic features and diagnosis of extraosseous plasmacytomas

Mon Dec 19 00:00:00 UTC 2011

Atypical carcinoid with prominent mucinous stroma: a hitherto unreported variant of pulmonary neuroendocrine tumor.

Endocrine pathology

Nannini N,Bertolini F,Cavazza A,Casali C,Mengoli MC,Rossi G

MA1-35249 was used in immunohistochemistry to report on a case of atypical carcinoid with prominent mucinous stroma

Tue Jun 01 00:00:00 UTC 2010

Primary plasmacytoma of the cervix in a 21-year-old female patient.

International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists

Schor AP,Moraes MP,Bisson FW,Bisson MA,Luiz OM,Bacchi CE

MA1-35249 was used in immunohistochemistry to report on a case of primary plasmacytoma of the cervix

Sat May 01 00:00:00 UTC 2010

Primary central nervous system peripheral T-cell lymphoma in a child.

Fetal and pediatric pathology

Gualco G,Wludarski S,Hayashi-Silva L,Medeiros Filho P,Veras G,Bacchi CE

MA1-35249 was used in immunohistochemistry to report on a case of primary central nervous system peripheral T-cell lymphoma in a child

Tue Sep 28 00:00:00 UTC 2010

Goblet cell carcinoid of the appendix: a clinicopathological and immunohistochemical study of 26 cases from southwest china.

International journal of surgical pathology

MA1-35249 was used in immunohistochemistry - paraffin section to report the clinicopathological and immunohistochemical features of goblet cell carcinoid

Wed Dec 01 00:00:00 UTC 2010

EGFR and K-ras mutations along the spectrum of pulmonary epithelial tumors of the lung and elaboration of a combined clinicopathologic and molecular scoring system to predict clinical responsiveness to EGFR inhibitors.

American journal of clinical pathology

Sartori G,Cavazza A,Sgambato A,Marchioni A,Barbieri F,Longo L,Bavieri M,Murer B,Meschiari E,Tamberi S,Cadioli A,Luppi F,Migaldi M,Rossi G

MA1-35249 was used in immunohistochemistry to develop a combined clinicopathologic and molecular scoring system for assessing the tumor responses to EGFR inhibitors

Wed Apr 01 00:00:00 UTC 2009

Composite lymphoma: EBV-positive classic Hodgkin lymphoma and peripheral T-cell lymphoma: a case report.

Applied immunohistochemistry & molecular morphology : AIMM

Gualco G,Chioato L,Van Den Berg A,Weiss LM,Bacchi CE

MA1-35249 was used in immunohistochemistry to report on a case of composite EBV-positive classic Hodgkin lymphoma and peripheral T-cell lymphoma

Thu Jan 01 00:00:00 UTC 2009

TTF-1, cytokeratin 7, 34betaE12, and CD56/NCAM immunostaining in the subclassification of large cell carcinomas of the lung.

American journal of clinical pathology

Rossi G,Marchioni A,Milani M,Scotti R,Foroni M,Cesinaro A,Longo L,Migaldi M,Cavazza A

MA1-35249 was used in immunohistochemistry to study the use of a number of immunohistochemical markers for the subclassification of large cell carcinomas of the lung

Wed Dec 01 00:00:00 UTC 2004

The prognostic role of c-kit protein expression in resected large cell neuroendocrine carcinoma of the lung.

The Annals of thoracic surgery

Casali C,Stefani A,Rossi G,Migaldi M,Bettelli S,Parise A,Morandi U

MA1-35249 was used in immunohistochemistry to study the prognostic value of c-kit protein expression in resected large cell neuroendocrine carcinoma

Thu Jan 01 00:00:00 UTC 2004

Desmoplastic and spindle cell melanomas express protein markers of the neural crest but not of later committed stages of Schwann cell differentiation.

Journal of cutaneous pathology

Huttenbach Y,Prieto VG,Reed JA

MA1-35249 was used in immunohistochemistry to investigate the expression of unique protein markers in desmoplastic and spindle cell melanomas

Tue Oct 01 00:00:00 UTC 2002

Bioinformatics

Protein Aliases: antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; N CAM1; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM

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Gene Aliases: CD56; MSK39; NCAM; NCAM1

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UniProt ID: (Human) P13591

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Entrez Gene ID: (Human) 4684

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Function(s)

virus receptor activity Ras guanyl-nucleotide exchange factor activity

Process(es)

MAPK cascade cell adhesion axon guidance positive regulation of GTPase activity viral entry into host cell regulation of synaptic plasticity interferon-gamma-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD56 Monoclonal Antibody (123C3)

CD56 Antibody (MA1-35249) in ICC/IF

Product Details

MA1-35249

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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