Invitrogen

CD56 Monoclonal Antibody (MEM-188)

CD56 Antibody in Immunocytochemistry (ICC/IF)

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CD56 Antibody (MA1-19129) in ICC/IF

Immunofluorescence analysis of CD56 was performed using 70% confluent log phase IMR-32 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD56 Monoclonal Antibody (MEM-188) (Product # MA1-19129) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Produ... View More

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CD56 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-CD56 Monoclonal Antibody (MEM-188) (Product # MA1-19129), shows increased expression of CD56 in IMR-32 as compared to HepG2. {RE}

Product Details

MA1-19129

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Immunocytochemistry (ICC/IF)

Assay-dependent

Flow Cytometry (Flow)

3 µg/mL

View 13 publications 13 publications

Immunoprecipitation (IP)

Assay-dependent

Neutralization (Neu)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Non-human primate

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

MEM-188

Immunogen

KG-1 human acute myelogenous cell line.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1073124

Product Specific Information

This antibody reacts with an extracellular epitope on a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.

Antigen-mediated heat retrieval in citrate buffer is required for staining of paraffin-embedded sections.

Target Information

CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa. CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity. Polysialic modification results in reduction of CD56-mediated cell adhesion. Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins. CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Differences in Peripheral Blood Lymphocytes between Brand-Name and Generic Tacrolimus Used in Stable Liver Transplant Recipients.

Medical principles and practice : international journal of the Kuwait University, Health Science Centre

Kim JM,Kwon CHD,Joh JW,Sinn DH,Choi GS,Park JB,Kang ES,Lee SK

MA119129 was used in flow cytometry to characterize the periferal blood lymphocytes phenotype in tacrolimus-treated liver transplanted patients.

Mon Mar 19 00:00:00 UTC 2018

Effect of Anti-IL-15 Administration on T Cell and NK Cell Homeostasis in Rhesus Macaques.

Journal of immunology (Baltimore, Md. : 1950)

DeGottardi MQ,Okoye AA,Vaidya M,Talla A,Konfe AL,Reyes MD,Clock JA,Duell DM,Legasse AW,Sabnis A,Park BS,Axthelm MK,Estes JD,Reiman KA,Sekaly RP,Picker LJ

MA119129 was used in flow cytometry to report the effect of IL-15 inhibition on T and natural killer cell dynamics in rhesus macaques

Mon Aug 15 00:00:00 UTC 2016

LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.

Nature communications

Lu XJ,Chen Q,Rong YJ,Yang GJ,Li CH,Xu NY,Yu CH,Wang HY,Zhang S,Shi YH,Chen J

MA119129 was used in flow cytometry to find that leukocyte cell-derived chemotaxin 2 promotes expansion and mobilization of hematopoietic stem cells

Tue Sep 06 00:00:00 UTC 2016

New IDH1 mutant inhibitors for treatment of acute myeloid leukemia.

Nature chemical biology

Okoye-Okafor UC,Bartholdy B,Cartier J,Gao EN,Pietrak B,Rendina AR,Rominger C,Quinn C,Smallwood A,Wiggall KJ,Reif AJ,Schmidt SJ,Qi H,Zhao H,Joberty G,Faelth-Savitski M,Bantscheff M,Drewes G,Duraiswami C,Brady P,Groy A,Narayanagari SR,Antony-Debre I,Mitchell K,Wang HR,Kao YR,Christopeit M,Carvajal L,Barreyro L,Paietta E,Makishima H,Will B,Concha N,Adams ND,Schwartz B,McCabe MT,Maciejewski J,Verma A,Steidl U

MA1-19129 was used in flow cytometry to assess the effects of allosteric inhibitors on different mutant forms of isocitrate dehydrogenase 1 in leukemia

Sun Nov 01 00:00:00 UTC 2015

Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.

Arthritis & rheumatology (Hoboken, N.J.)

Van Eyck L,De Somer L,Pombal D,Bornschein S,Frans G,Humblet-Baron S,Moens L,de Zegher F,Bossuyt X,Wouters C,Liston A

MA1-19129 was used in Flow cytometry/Cell sorting to determine the underlying genetic defect responsible for severe early onset and refractory systemic lupus erythematosus.

Mon Jun 01 00:00:00 UTC 2015

Impaired Th17 polarization of phenotypically naive CD4(+) T-cells during chronic HIV-1 infection and potential restoration with early ART.

Retrovirology

DaFonseca S,Niessl J,Pouvreau S,Wacleche VS,Gosselin A,Cleret-Buhot A,Bernard N,Tremblay C,Jenabian MA,Routy JP,Ancuta P

MA119129 was used in flow cytometry to elucidate Th17 cell polarization, depletion, and restoration in response to HIV infection and antiretroviral therapy

Thu Apr 30 00:00:00 UTC 2015

Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1.

PloS one

Grieco AJ,Billett HH,Green NS,Driscoll MC,Bouhassira EE

MA1-19129 was used in flow cytometry to elucidate regulation of γ-globin expression in F-cells.

Mon Feb 29 00:00:00 UTC 2016

Grb10 deletion enhances muscle cell proliferation, differentiation and GLUT4 plasma membrane translocation.

Journal of cellular physiology

Mokbel N,Hoffman NJ,Girgis CM,Small L,Turner N,Daly RJ,Cooney GJ,Holt LJ

MA1-19129 was used in flow cytometry to study the increased muscle cell differentiation/proliferation and plama membrane translocation of Glut4 in mice deleted for Grb10

Sat Nov 01 00:00:00 UTC 2014

The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells.

Journal of cellular and molecular medicine

Elsner L,Flügge PF,Lozano J,Muppala V,Eiz-Vesper B,Demiroglu SY,Malzahn D,Herrmann T,Brunner E,Bickeböller H,Multhoff G,Walter L,Dressel R

MA1-19129 was used in flow cytometry to report that HSP70 activates human NK cells to kill target cells expressing MICA in a natural killer group 2 member D-dependent manner

Thu Apr 01 00:00:00 UTC 2010

A new protocol for multiple inhalation of IFN-gamma successfully treats MDR-TB: a case study.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease

Grahmann PR,Braun RK

MA119129 was used in flow cytometry to demonstrate that aerosolized interferon-gamma results in the transient conversion of sputum smears in multidrug-resistant pulmonary tuberculosis

Sun Jun 01 00:00:00 UTC 2008

Adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells involves CD44.

Cells, tissues, organs

Wagner W,Wein F,Roderburg C,Saffrich R,Diehlmann A,Eckstein V,Ho AD

MA1-19129 was used in blocking or activating experiment to test whether CD44 aids in adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells

Fri Sep 19 00:00:00 UTC 2008

Unique characteristics of NK cells throughout the human female reproductive tract.

Clinical immunology (Orlando, Fla.)

Mselle TF,Meadows SK,Eriksson M,Smith JM,Shen L,Wira CR,Sentman CL

MA1-19129 was used in flow cytometry to characterize natural killer cells from different tissues of the FRT

Sun Jul 01 00:00:00 UTC 2007

Isolation of large numbers of mesenchymal stem cells from the washings of bone marrow collection bags: characterization of fresh mesenchymal stem cells.

Transplantation

Mageed AS,Pietryga DW,DeHeer DH,West RA

MA1-19129 was used in flow cytometry to test if the normally discarded bone marrow collection kits are a convenient source of large numbers of mesenchymal stem cells

Fri Apr 27 00:00:00 UTC 2007

TLRs mediate IFN-gamma production by human uterine NK cells in endometrium.

Journal of immunology (Baltimore, Md. : 1950)

Eriksson M,Meadows SK,Basu S,Mselle TF,Wira CR,Sentman CL

MA1-19129 was used in flow cytometry to assess TLR expression and signaling by uterine natural killer cells from the human endometrium

Mon May 15 00:00:00 UTC 2006

Phenotypic and functional differences between human saphenous vein (HSVEC) and umbilical vein (HUVEC) endothelial cells.

Atherosclerosis

Tan PH,Chan C,Xue SA,Dong R,Ananthesayanan B,Manunta M,Kerouedan C,Cheshire NJ,Wolfe JH,Haskard DO,Taylor KM,George AJ

MA1-19129 was used in flow cytometry to compare human saphenous vein endothelial cells with human umbilical vein endothelial cells

Thu Apr 01 00:00:00 UTC 2004

Molecular characterization of human 4Ig-B7-H3, a member of the B7 family with four Ig-like domains.

Journal of immunology (Baltimore, Md. : 1950)

Steinberger P,Majdic O,Derdak SV,Pfistershammer K,Kirchberger S,Klauser C,Zlabinger G,Pickl WF,Stöckl J,Knapp W

MA1-19129 was used in flow cytometry to characterize B7-H3.

Sun Feb 15 00:00:00 UTC 2004

LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.

The Journal of experimental medicine

Brdicková N,Brdicka T,Angelisová P,Horváth O,Spicka J,Hilgert I,Paces J,Simeoni L,Kliche S,Merten C,Schraven B,Horejsí V

MA1-19129 was used in flow cytometry to describe a new raft-associated adaptor protein, Lck-interacting molecule, expressed predominantly in T lymphocytes.

Mon Nov 17 00:00:00 UTC 2003

Trafficking machinery of NKT cells: shared and differential chemokine receptor expression among V alpha 24(+)V beta 11(+) NKT cell subsets with distinct cytokine-producing capacity.

Blood

Kim CH,Johnston B,Butcher EC

MA1-19129 was used in flow cytometry to report differential expression of chemokine receptors among NKT cell subsets.

Mon Jul 01 00:00:00 UTC 2002

Identity of Leu-19 (CD56) leukocyte differentiation antigen and neural cell adhesion molecule.

The Journal of experimental medicine

Lanier LL,Testi R,Bindl J,Phillips JH

MA1-19129 was used in immunoprecipitation to propose that the Leu-19 antigen on leukocytes contributes to cell adhesion and is analogous to N-CAM on neural cells.

Thu Jun 01 00:00:00 UTC 1989

Bioinformatics

Protein Aliases: antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; N CAM1; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM

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Gene Aliases: CD56; MSK39; NCAM; NCAM1

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UniProt ID: (Human) P13591

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Entrez Gene ID: (Human) 4684

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Function(s)

virus receptor activity Ras guanyl-nucleotide exchange factor activity

Process(es)

MAPK cascade cell adhesion axon guidance positive regulation of GTPase activity viral entry into host cell regulation of synaptic plasticity interferon-gamma-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD56 Monoclonal Antibody (MEM-188)

CD56 Antibody (MA1-19129) in ICC/IF

Product Details

MA1-19129

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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