Invitrogen

CD58 Monoclonal Antibody (TS2/9)

CD58 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

CD58 Antibody (MA5800) in ICC/IF

Immunofluorescent analysis of CD58 (green) showing staining in the cytoplasm of Raji cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD58 monoclonal antibody (Product # MA5800) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated se... View More

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Product Details

MA5800

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Neutralization (Neu)

Assay-dependent

View 2 publications 2 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TS2/9

Immunogen

Human Leukocyte Function Antigen-3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_223518

Product Specific Information

MA5800 targets CD58 in WB, FACS, IF, IHC(F), IP, and Neu applications and shows reactivity with Human and Mouse samples.

The MA5800 immunogen is human Leukocyte Function Antigen-3.

MA5800 detects CD58 which has a predicted molecular weight of approximately 24 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Endoplasmic Reticulum (ER) Reorganization and Intracellular Retention of CD58 Are Functionally Independent Properties of the Human Cytomegalovirus ER-Resident Glycoprotein UL148.

Journal of virology

Nguyen CC,Domma AJ,Zhang H,Kamil JP

MA5800 was used in Western Blotting to demonstrate that the potential of UL148 to reorganize the ER and to retain CD58 are separable by mutagenesis and possibly, by evolution, since chimpanzee cytomegalovirus UL148 retains CD58 but does not remodel the ER.

Fri Feb 14 00:00:00 UTC 2020

Nurse-like cells promote CLL survival through LFA-3/CD2 interactions.

Oncotarget

Boissard F,Tosolini M,Ligat L,Quillet-Mary A,Lopez F,Fournié JJ,Ysebaert L,Poupot M

MA5800 was used in Functional assay to investigate the expression of various receptor/ligand pairs at the surface of chronic lymphocytic leukaemia nurse-like cells.

Tue Aug 08 00:00:00 UTC 2017

Identification and purification of classical Hodgkin cells from lymph nodes by flow cytometry and flow cytometric cell sorting.

American journal of clinical pathology

Fromm JR,Kussick SJ,Wood BL

MA5800 was used in blocking/activating experiment to investigate the reliability of FACS for the isolation of classical Hodgkin cells

Wed Nov 01 00:00:00 UTC 2006

ICOS-ligand, expressed on human endothelial cells, costimulates Th1 and Th2 cytokine secretion by memory CD4+ T cells.

Proceedings of the National Academy of Sciences of the United States of America

Khayyamian S,Hutloff A,Büchner K,Gräfe M,Henn V,Kroczek RA,Mages HW

MA5800 was used in ELISA to investigate the function of ICOS-ligand on human endothelial cells

Tue Apr 30 00:00:00 UTC 2002

Human lung microvascular endothelial cells activate allogeneic T cells through an LFA-3-dependent, but CD86-independent mechanism.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Hansen AB,Bouchelouche K,Olesen JD

MA5800 was used in immunocytochemistry to investigate the mechanism for the allogeneic T cell activation by human lung microvascular endothelial cells

Sat Dec 01 00:00:00 UTC 2001

CD4+ T cell and eosinophil adhesion is mediated by specific ICAM-3 ligation and results in eosinophil activation.

Journal of immunology (Baltimore, Md. : 1950)

Douglas IS,Leff AR,Sperling AI

MA5800 was used in flow cytometry to study the mechanism for the T cell and eosinophil adhesion and activation

Wed Mar 15 00:00:00 UTC 2000

AD2, a human molecule involved in the interaction of T cells with epidermal keratinocytes and thymic epithelial cells.

Journal of immunology (Baltimore, Md. : 1950)

Bruggers CS,Patel DD,Scearce RM,Whichard LP,Haynes BF,Singer KH

MA5800 was used in blocking/activating experiment to investigate the importance of AD2 in the interaction between T cells and epidermal keratinocytes and thymic epithelial cells

Wed Mar 01 00:00:00 UTC 1995

Differential expression of cell adhesion molecules CD54/CD11a and CD58/CD2 by human melanoma cells and functional role in their interaction with cytotoxic cells.

Cancer research

Altomonte M,Gloghini A,Bertola G,Gasparollo A,Carbone A,Ferrone S,Maio M

MA5800 was used in immunohistochemistry to study the cell adhesion molecules CD54/CD11a and CD58/CD2 in human melanoma cells

Thu Jul 15 00:00:00 UTC 1993

Bioinformatics

Protein Aliases: Ag3; CD305; CD58; CD58 antigen, (lymphocyte function-associated antigen 3); FLJ23181; FLJ43722; LAIR-1; LAIR1; Lymphocyte function-associated antigen 3; RP5-1086K13.2; Surface glycoprotein LFA-3

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Gene Aliases: ag3; CD58; LFA-3; LFA3

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UniProt ID: (Human) P19256

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Entrez Gene ID: (Human) 965

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Function(s)

receptor binding protein binding

Process(es)

cell adhesion single organismal cell-cell adhesion heterotypic cell-cell adhesion leukocyte migration cellular response to interferon-gamma cellular response to tumor necrosis factor positive regulation of interleukin-8 secretion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD58 Monoclonal Antibody (TS2/9)

CD58 Antibody (MA5800) in ICC/IF

Product Details

MA5800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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