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Invitrogen
CD59 Recombinant Rabbit Monoclonal Antibody (5W9W7)
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FIGURE: 1 / 3
CD59 Antibody (MA5-35440) in IHC (P)
Immunohistochemistry analysis of CD59 in paraffin-embedded mouse testis. Samples were incubated with CD59 Monoclonal antibody (Product # MA5-35440) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
MA5-35440
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5W9W7
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 49-128 of human CD59 (NP_0006021)
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849341
Product Specific Information
Immunogen sequence: DACLITKAGL QVYNKCWKFE HCNFNDVTTR LRENELTYYC CKKDLCNFNE QLENGGTSLS EKTVLLLVTP FLAAAWSLHP
Target Information
CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). CD59 is the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 also associates with C5b-8 complex and counteracts appropriate formation of cytolytic pore within the plasma membrane. Further, CD59 is a low-affinity ligand of human CD2, causes T cell costimulation, and is involved in lymphocyte signal transduction. CD59 is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. Mutations in this gene cause CD59 deficiency, a disease involving hemolytic anemia, thrombosis, and cerebral infarction. Multiple alternatively spliced transcript variants of CD59 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1F5 antigen; 20 kDa homologous restriction factor; Cb59b molecule; CD59; CD59 antigen; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); CD59 glycoprotein; CD59 molecule, complement regulatory protein; CD59A glycoprotein; CD59B glycoprotein; CD59b moleucle, complement regulatory protein; HRF-20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MAC-inhibitory protein; MAC-IP; MACIF; MEM43 antigen; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane inhibitor of reactive lysis; MIRL; Protectin; surface anitgen recognized by monoclonal antibody 16.3A5; T cell-activating protein
Gene Aliases: 16.3A5; 1F5; AA987121; CD59; Cd59a; Cd59b; EJ16; EJ30; EL32; G344; HRF-20; HRF20; MAC-IP; MACIF; MACIP; MEM43; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20; protectin
UniProt ID: (Human) P13987, (Rat) P27274, (Mouse) O55186, (Mouse) P58019
Entrez Gene ID: (Human) 966, (Rat) 25407, (Mouse) 12509, (Mouse) 333883
negative regulation of activation of membrane attack complex
cell surface receptor signaling pathway
negative regulation of angiogenesis
negative regulation of vascular endothelial growth factor receptor signaling pathway
negative regulation of complement activation
negative regulation of fibroblast growth factor production
positive regulation of T cell proliferation
negative regulation of apoptotic process
negative regulation of cytolysis
cell activation
ER to Golgi vesicle-mediated transport
blood coagulation
regulation of complement activation
COPII vesicle coating
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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