Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (HM beta 3.1), FITC

View all (100) CD61 (Integrin beta 3) antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-28176

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Neat-1:10
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

HM beta 3.1

Immunogen

Mouse alpha 5 beta 3 protein purified from the mouse hybridoma 2B4
View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

100 µg/mL

Purification

Protein G

Storage buffer

PBS with 1% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2745152

Product Specific Information

This antibody recognizes the murine integrin beta 3 subunit (CD61), approximately a 90 kDa a type I membrane protein, expressed primarily on megakaryocytes, platelets, monocytes, macrophages, granulocytes and endothelial cells. CD61 associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) to form the platelet glycoprotein complex IIb/IIIa and the vitronectin receptor (VNR) respectively.

Target Information

CD61 (GPIIIa, ITGB3) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. CD61 is a 105 kDa glycoprotein that associates with either the alpha 11b integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, Von Willebrand Factor, vitronectin and thrombospondin. The CD61 protein product is composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. CD61 is found along with the alpha IIb chain in platelets. Like other integrins, CD61 is known to participate in cell adhesion as well as cell-surface mediated signaling. Diseases associated with CD61 dysfunction include Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

References (0)

Have you cited this product in a publication?
Let us know so we can reference it here.
Cite this product

Bioinformatics

Protein Aliases: CD61; GPIIIa; Integrin beta-3; platelet gpIIIa; Platelet membrane glycoprotein IIIa

View more View less

Gene Aliases: CD61; GP3A; INGRB3; Itgb3

View more View less

UniProt ID: (Mouse) O54890

View more View less

Entrez Gene ID: (Mouse) 16416

View more View less

Function(s)
fibronectin binding protease binding protein disulfide isomerase activity receptor activity integrin binding protein binding peptide binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding extracellular matrix binding fibrinogen binding
Process(es)
positive regulation of endothelial cell proliferation positive regulation of leukocyte migration protein folding cellular calcium ion homeostasis cell-substrate junction assembly cell adhesion cell-matrix adhesion integrin-mediated signaling pathway regulation of G-protein coupled receptor protein signaling pathway positive regulation of endothelial cell migration negative regulation of macrophage derived foam cell differentiation positive regulation of fibroblast migration negative regulation of lipid storage smooth muscle cell migration positive regulation of smooth muscle cell migration cell growth cell migration platelet activation regulation of cell migration positive regulation of cell migration cell-substrate adhesion activation of protein kinase activity negative regulation of lipid transport positive regulation of cell adhesion mediated by integrin positive regulation of osteoblast proliferation heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading apolipoprotein A-I-mediated signaling pathway positive regulation of MAPK cascade positive regulation of osteoclast differentiation negative regulation of low-density lipoprotein particle receptor biosynthetic process positive regulation of angiogenesis positive regulation of bone resorption viral entry into host cell positive regulation of smooth muscle cell proliferation cell projection morphogenesis positive regulation of peptidyl-tyrosine phosphorylation negative regulation of lipoprotein metabolic process negative chemotaxis regulation of release of sequestered calcium ion into cytosol angiogenesis involved in wound healing negative regulation of cell death positive regulation of ERK1 and ERK2 cascade platelet aggregation positive regulation of glomerular mesangial cell proliferation positive regulation of adenylate cyclase-inhibiting opioid receptor signaling pathway
guarantee_icon

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more
help_icon

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support