Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (VI-PL2), eBioscience™

4 Published Figures
3 References
View all (108) CD61 (Integrin beta 3) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

14-0619-82

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-Dependent
-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent
-

Flow Cytometry (Flow)

0.125 µg/test
View 3 publications 3 publications

Functional Assay (Functional)

Assay-Dependent
-
Product Specifications

Species Reactivity

Baboon, Dog, Cynomolgus monkey, Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

VI-PL2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_953606

Product Specific Information

Description: The VI-PL2 monoclonal antibody reacts with human CD61, also known as integrin beta 3 and GPIIIa. CD61 is a 90-110 kDa member of the beta integrin family expressed by a wide variety of cells, including leukocytes, platelets, endothelial and smooth muscle cells. CD61 binds non-covalently with the alpha integrins CD41 and CD51, to form the alpha IIb beta3 (CD41/CD61) and alpha v beta3 (CD51/CD61) complexes. These alpha beta heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. The CD41/CD61 integrin has been shown to be involved in platelet aggregation, and binds to fibrinogen, von Willebrand Factor (vWF) and fibronectin. The CD51/CD61 integrin binds to matrix proteins including vitronectin, fibronectin, vWF and fibrinogen, and has been shown to have a strong role in modulating the migration and survival of angiogenic endothelial cells.

This VI-PL2 antibody has also been shown to crossreact with canine and several non-human primate species, including baboon, rhesus, and cynomologus monkey.

Applications Reported: This VI-PL2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistochemical staining, and functional assays.

Applications Tested: This VI-PL2 antibody has been tested by flow cytometric analysis of human peripheral blood platelets. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD61, also known as GPIIIa or ITGB3, is a 105 kDa glycoprotein expressed on activated T cells, granulocytes, megakaryocytes, platelets, and their precursors. It plays a crucial role in platelet aggregation and functions as a receptor for fibrinogen, fibronectin, von Willebrand factor, vitronectin, and thrombospondin. CD61 forms heterodimeric complexes by associating non-covalently with integrin alpha subunits: alphaV (CD51) to create the Vitronectin Receptor and alphaIIb (CD41) to form gpIIb/IIIa. These complexes are responsible for adhesion to extracellular matrix components, facilitating cell adhesion and cell-surface mediated signaling. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, and osteoclasts in association with CD51. The protein product of CD61 is composed of an alpha chain and a beta chain, which can combine with multiple partners to form different integrins. Its involvement in cell adhesion and signaling underscores its importance in normal physiological processes. Dysfunction of CD61 is associated with diseases such as Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder, highlighting its critical role in hemostasis and platelet function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: antigen CD61; CD61; glycoprotein GPIIIa; GPIIIa; hypothetical protein; integrin beta 3; Integrin beta-3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); Platelet membrane glycoprotein IIIa

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Gene Aliases: BDPLT16; BDPLT2; CD61; EGM_08034; GP3A; GPIIIA; GT; ITGB3

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UniProt ID: (Human) P05106

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Entrez Gene ID: (Human) 3690, (Cynomolgus monkey) 102117791, (Dog) 403788, (Rhesus monkey) 717379

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Function(s)
virus receptor activity fibronectin binding protease binding protein disulfide isomerase activity platelet-derived growth factor receptor binding protein binding coreceptor activity fibroblast growth factor binding enzyme binding C-X3-C chemokine binding insulin-like growth factor I binding neuregulin binding identical protein binding vascular endothelial growth factor receptor 2 binding cell adhesion molecule binding extracellular matrix binding
Process(es)
positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation platelet degranulation cell-substrate junction assembly cell adhesion cell-matrix adhesion integrin-mediated signaling pathway blood coagulation positive regulation of endothelial cell migration negative regulation of macrophage derived foam cell differentiation negative regulation of lipid storage smooth muscle cell migration cell growth cell migration platelet activation extracellular matrix organization positive regulation of vascular endothelial growth factor receptor signaling pathway cell-substrate adhesion activation of protein kinase activity negative regulation of lipid transport heterotypic cell-cell adhesion substrate adhesion-dependent cell spreading tube development apolipoprotein A-I-mediated signaling pathway wound healing regulation of bone resorption negative regulation of low-density lipoprotein particle receptor biosynthetic process viral entry into host cell vascular endothelial growth factor receptor signaling pathway mesodermal cell differentiation positive regulation of peptidyl-tyrosine phosphorylation negative regulation of lipoprotein metabolic process leukocyte migration negative chemotaxis angiogenesis involved in wound healing platelet aggregation
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