Invitrogen

CD62E (E-selectin) Monoclonal Antibody (P2H3), PE, eBioscience™

CD62E (E-selectin) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

CD62E (E-selectin) Antibody (12-0627-42) in Flow

Human Umbilical Vein Endothelial Cells (HUVEC) were activated with Human TNF alpha Recombinant Protein (Product # 14-8329-81) and stained with Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (open histogram) or Anti-Human CD62E (E-Selectin) PE (filled histogram). Total viable cells were used for analysis.

Product Image Gallery

Product Details

12-0627-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

P2H3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11219468

Product Specific Information

Description: The P2H3 monoclonal antibody reacts with human CD62E, a 97-115 kDa member of the selectin family. CD62E, also known as E-selectin or endothelial-leukocyte adhesion molecule-1 (ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines including TNFalpha and IL-1beta. Induced expression of CD62E during inflammatory conditions is thought to mediate leukocyte rolling including the initial interaction of neutrophils with endothelium.

The P2H3 monoclonal antibody also inhibits cellular adhesion to cytokine-activated endothelial cells.

Applications Reported: This P2H3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This P2H3 antibody has been pre-titrated and tested by flow cytometric analysis of TNF alpha-actiavted Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Development of an In Vitro Blood Vessel Model Using Autologous Endothelial Cells Generated from Footprint-Free hiPSCs to Analyze Interactions of the Endothelium with Blood Cell Components and Vascular Implants.

Cells

Weber J,Weber M,Feile A,Schlensak C,Avci-Adali M

12-0627-42 was used in Flow cytometry/Cell sorting to show that in the future, this blood vessel-like model could serve as an in vitro platform to test the influence of vascular implants and coatings on endothelialization and to analyze the interaction of the endothelium with blood cell components.

Sat Apr 22 00:00:00 UTC 2023

Cells

Weber J,Weber M,Feile A,Schlensak C,Avci-Adali M

Sat Apr 22 00:00:00 UTC 2023

Cells

Weber J,Weber M,Feile A,Schlensak C,Avci-Adali M

Sat Apr 22 00:00:00 UTC 2023

Serum of Post-COVID-19 Syndrome Patients with or without ME/CFS Differentially Affects Endothelial Cell Function In Vitro.

Cells

Flaskamp L,Roubal C,Uddin S,Sotzny F,Kedor C,Bauer S,Scheibenbogen C,Seifert M

12-0627-42 was used in Flow cytometry/Cell sorting to investigate the effects of patients' sera on endothelia cells (ECs) in vitro.

Tue Aug 02 00:00:00 UTC 2022

Colonization of dermal arterioles by Neisseria meningitidis provides a safe haven from neutrophils.

Nature communications

Manriquez V,Nivoit P,Urbina T,Echenique-Rivera H,Melican K,Fernandez-Gerlinger MP,Flamant P,Schmitt T,Bruneval P,Obino D,Duménil G

12-0627-42 was used in Flow Cytometry to demonstrate how neutrophil recruitment contributes to resolution of Neisseria meningitidis in a humanized mouse model.

Tue Jul 27 00:00:00 UTC 2021

Expansion of functional personalized cells with specific transgene combinations.

Nature communications

Lipps C,Klein F,Wahlicht T,Seiffert V,Butueva M,Zauers J,Truschel T,Luckner M,Köster M,MacLeod R,Pezoldt J,Hühn J,Yuan Q,Müller PP,Kempf H,Zweigerdt R,Dittrich-Breiholz O,Pufe T,Beckmann R,Drescher W,Riancho J,Sañudo C,Korff T,Opalka B,Rebmann V,Göthert JR,Alves PM,Ott M,Schucht R,Hauser H,Wirth D,May T

12-0627 was used in Flow cytometry/Cell sorting to develop a novel strategy for rapid, functional, personalised cell expansion.

Thu Mar 08 00:00:00 UTC 2018

Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.

PloS one

An N,Andrukhov O,Tang Y,Falkensammer F,Bantleon HP,Ouyang X,Rausch-Fan X

12-0627 was used in Flow cytometry/Cell sorting to show that high concentrations of nicotine inhibit the inflammatory response of endothelial cells, contributing to cardiovascular disease progression.

Mon Jun 29 00:00:00 UTC 2015

Lipopolysaccharide and sphingosine-1-phosphate cooperate to induce inflammatory molecules and leukocyte adhesion in endothelial cells.

Journal of immunology (Baltimore, Md. : 1950)

Fernández-Pisonero I,Dueñas AI,Barreiro O,Montero O,Sánchez-Madrid F,García-Rodríguez C

12-0627 was used in Flow cytometry/Cell sorting to show that sphingosine-1-phosphate and LPS (a TLR4 ligand) work together to increase proinflammatory molecules in endothelial cells, inducing leukocyte adhesion.

Sat Dec 01 00:00:00 UTC 2012

Detection and monitoring of the multiple inflammatory responses by photoacoustic molecular imaging using selectively targeted gold nanorods.

Biomedical optics express

Ha S,Carson A,Agarwal A,Kotov NA,Kim K

12-0627 was used in Flow cytometry to study the upregulation of intercellular adhesion molecule-1 and E-selectin in human umbilical vein endothelial cells, following inflammatory stimulation.

Wed Feb 23 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: CD62 antigen-like family member E; CD62E; e selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2

View more View less

Gene Aliases: CD62E; ELAM; ELAM1; ESEL; LECAM2; SELE

View more View less

UniProt ID: (Human) P16581

View more View less

Entrez Gene ID: (Human) 6401

View more View less

Function(s)

transmembrane signaling receptor activity protein binding sialic acid binding phospholipase binding oligosaccharide binding

Process(es)

positive regulation of receptor internalization leukocyte migration involved in inflammatory response inflammatory response heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion activation of phospholipase C activity calcium-mediated signaling actin filament-based process response to lipopolysaccharide response to tumor necrosis factor regulation of inflammatory response leukocyte migration leukocyte tethering or rolling response to interleukin-1

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD62E (E-selectin) Monoclonal Antibody (P2H3), PE, eBioscience™

CD62E (E-selectin) Antibody (12-0627-42) in Flow

Product Details

12-0627-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!