Invitrogen

CD62E (E-selectin) Monoclonal Antibody (P2H3), eBioscience™

CD62E (E-selectin) Antibody in Western Blot (WB)

FIGURE: 1 / 5

CD62E (E-selectin) Antibody (14-0627-82) in WB

Western blot was performed using Anti-CD62E (E-selectin) Monoclonal Antibody (Product # 14-0627-82) and 67, 115 kDa bands corresponding to CD62E were observed across the tissues tested. Tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Mouse Spleen (Lane 2) and Mouse White Adipose (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed... View More

CD62E (E-selectin) Antibody in Flow Cytometry (Flow)

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Product Details

14-0627-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Immunohistochemistry (IHC)

Assay-Dependent

Flow Cytometry (Flow)

1 µg/test

View 3 publications 3 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Inhibition Assays (IA)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

P2H3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1210768

Product Specific Information

Description: The P2H3 monoclonal antibody reacts with human CD62E, a 97-115 kDa member of the selectin family. CD62E, also known as E-selectin or endothelial-leukocyte adhesion molecule-1 (ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines including TNFalpha and IL-1beta. Induced expression of CD62E during inflammatory conditions is thought to mediate leukocyte rolling including the initial interaction of neutrophils with endothelium.

The P2H3 monoclonal antibody also inhibits cellular adhesion to cytokine-activated endothelial cells.

Applications Reported: This P2H3 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.

Applications Tested: This P2H3 antibody has been tested by flow cytometric analysis of TNF alpha-actiavted Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Development of an In Vitro Blood Vessel Model Using Autologous Endothelial Cells Generated from Footprint-Free hiPSCs to Analyze Interactions of the Endothelium with Blood Cell Components and Vascular Implants.

Cells

Weber J,Weber M,Feile A,Schlensak C,Avci-Adali M

Published figure using CD62E (E-selectin) monoclonal antibody (Product # 14-0627-82) in Flow Cytometry

Sat Apr 22 00:00:00 UTC 2023

Surgical and intravital microscopy protocol to image Trypanosoma brucei-host interactions in live rodent models.

STAR protocols

De Niz M,Figueiredo LM

14-0627-82 was used in inhibition assays to provide an IVM protocol for time-lapse imaging of dynamic Trypanosoma brucei-host interactions in ten mammalian organs and in systemic circulation.

Fri Jun 17 00:00:00 UTC 2022

A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy.

EMBO molecular medicine

Chidiac R,Abedin M,Macleod G,Yang A,Thibeault PE,Blazer LL,Adams JJ,Zhang L,Roehrich H,Jo HN,Seshagiri S,Sidhu SS,Junge HJ,Angers S

14-0627-82 was used in Functional assay to develop F4L5.13, a tetravalent antibody designed to induce FZD4 and LRP5 proximity in such a way as to trigger βcatenin signaling.

Wed Jul 07 00:00:00 UTC 2021

Organotypic endothelial adhesion molecules are key for Trypanosoma brucei tropism and virulence.

Cell reports

De Niz M,Brás D,Ouarné M,Pedro M,Nascimento AM,Henao Misikova L,Franco CA,Figueiredo LM

14-0627-82 was used in Inhibition Assays to investigate the importance of the vasculature in tissue tropism.

Tue Sep 21 00:00:00 UTC 2021

Colonization of dermal arterioles by Neisseria meningitidis provides a safe haven from neutrophils.

Nature communications

Manriquez V,Nivoit P,Urbina T,Echenique-Rivera H,Melican K,Fernandez-Gerlinger MP,Flamant P,Schmitt T,Bruneval P,Obino D,Duménil G

14-0627-82 was used in Flow Cytometry to demonstrate how neutrophil recruitment contributes to resolution of Neisseria meningitidis in a humanized mouse model.

Tue Jul 27 00:00:00 UTC 2021

Long non‑coding RNA DILC is involved in sepsis by modulating the signaling pathway of the interleukin‑6/signal transducer and activator of transcription 3/Toll‑like receptor 4 axis.

Molecular medicine reports

Huang W,Huang L,Wen M,Fang M,Deng Y,Zeng H

14-0627-82 was used in ELISA to investigate the role of deleted in liver cancer 1 (DILC), interleukin (IL)‚6, signal transducer and activator of transcription 3 (STAT3), and Toll‚like receptor 4 (TLR4) in the pathogenesis of sepsis.

Sat Dec 01 00:00:00 UTC 2018

Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion.

Scientific reports

Stolfa G,Mondal N,Zhu Y,Yu X,Buffone A,Neelamegham S

14-0627 was used in Blocking experiments to develop a CRISPR-Cas9 toolkit to selectively truncate N-glycans, O-glycans and glycosphingolipids.

Tue Jul 26 00:00:00 UTC 2016

Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.

PloS one

An N,Andrukhov O,Tang Y,Falkensammer F,Bantleon HP,Ouyang X,Rausch-Fan X

Published figure using CD62E (E-selectin) monoclonal antibody (Product # 14-0627-82) in Flow Cytometry

Mon Jun 29 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: CD62 antigen-like family member E; CD62E; e selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2

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Gene Aliases: CD62E; E-selectin; ELAM; Elam-1; ELAM1; ESEL; LECAM2; SELE

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UniProt ID: (Human) P16581, (Mouse) Q00690

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Entrez Gene ID: (Human) 6401, (Mouse) 20339

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Function(s)

transmembrane signaling receptor activity protein binding sialic acid binding phospholipase binding oligosaccharide binding carbohydrate binding

Process(es)

positive regulation of receptor internalization leukocyte migration involved in inflammatory response inflammatory response heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion activation of phospholipase C activity calcium-mediated signaling actin filament-based process response to lipopolysaccharide response to tumor necrosis factor regulation of inflammatory response leukocyte migration leukocyte tethering or rolling response to interleukin-1 positive regulation of leukocyte migration cell adhesion signal transduction

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD62E (E-selectin) Monoclonal Antibody (P2H3), eBioscience™

CD62E (E-selectin) Antibody (14-0627-82) in WB

Product Details

14-0627-82

Western Blot (WB)

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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