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Invitrogen
CD62L (L-Selectin) Monoclonal Antibody (SK11), FITC, eBioscience™
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FIGURE: 1 / 1
CD62L (L-Selectin) Antibody (11-0625-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0048-42) and Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (left) or Anti-Human/NHP CD62L (L-selectin) FITC (right). Cells in the lymphocyte gate were used for analysis.
Product Details
11-0625-42
Product Specifications
Species Reactivity
Baboon,
Chimpanzee,
Cynomolgus monkey,
Human,
Non-human primate,
Rhesus monkey
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
SK11
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10597901
Product Specific Information
Description: This SK11 monoclonal antibody reacts with human and non-human primate CD62L (also known as L-selectin and LECAM). CD62L is expressed on neutrophils, monocytes, and subsets of T, B, and NK cells, as well as endothelial cells. This cell surface adhesion molecule binds glycolsylated, fucosylated and sulfated sialylated glycoproteins, including CD34, glycam-1, and MadCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.
The SK11 antibody has been reported to recognize chimpanzee, rhesus, cynomolgus monkey, olive baboon, and pigtailed macaque as well as pig.
Applications Reported: This SK11 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This SK11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD62 antigen-like family member L; CD62L; gp90-MEL; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; selectin E (endothelial adhesion molecule 1); selectin L; selectin L (lymphocyte adhesion molecule 1); TQ1
Gene Aliases: CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; SELE; SELL; TQ1
UniProt ID: (Rhesus monkey) Q95198, (Human) P14151, (Chimpanzee) Q95237
Entrez Gene ID: (Cynomolgus monkey) 102142895, (Rhesus monkey) 701419, (Human) 6402, (Chimpanzee) 450191
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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