Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Search All
Search button Close
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central

          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD63 Antibodies

          Proteintech

          CD63 Polyclonal Antibody

          258 References
          View all (139) CD63 antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD63 Polyclonal Antibody

          • Antibody Testing Data (15)
          CD63 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          CD63 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 15

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          CD63 Antibody (25682-1-AP) in ICC/IF

          Immunofluorescent analysis of ( -20°C Ethanol ) fixed A431 cells using 25682-1-AP (CD63 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          CD63 Antibody in Immunocytochemistry (ICC/IF)
          CD63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD63 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD63 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          CD63 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))
          CD63 Antibody in Western Blot (WB)
          CD63 Antibody in Western Blot (WB)

          Product Details

          25682-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:200-1:1,000
          View 228 publications 228 publications

          Immunohistochemistry (IHC)

          -
          View 6 publications 6 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          1:800
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:500
          View 12 publications 12 publications

          Flow Cytometry (Flow)

          Assay-dependent
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications

          Not applicable (N/A)

          -
          View 7 publications 7 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          CD63 Fusion Protein Ag19690 (104-209 aa encoded by BC002349)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.26 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: GYVFRDKVM SEFNNNFRQQ MENYPKNNHT ASILDRMQAD FKCCGAANYT DWEKIPSMSK NRVPDSCCIN VTVGCGINFN EKAIHKEGCV EKIGGWLRKN VLVVAAA (104-209 aa encoded by BC002349 )

          Target Information

          CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. CD63 is also present in the plasma membrane, usually following cell activation. Hence, CD63 has become a widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas. CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD 63; CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Granulophysin; LAMP-3; Limp1; Lysosomal-associated membrane protein 3; Lysosome integral membrane protein 1; lysosome-associated membrane glycoprotein 3; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; Tspan-30

          View more View less

          Gene Aliases: CD63; LAMP-3; ME491; MLA1; OMA81H; TSPAN30

          View more View less

          UniProt ID: (Human) P08962

          View more View less

          Entrez Gene ID: (Human) 967

          View more View less

          Function(s)
          protein binding protein complex binding
          Process(es)
          positive regulation of receptor internalization platelet degranulation cell-matrix adhesion negative regulation of epithelial cell migration protein transport cell migration epithelial cell differentiation cellular protein localization endosome to melanosome transport positive regulation of cell adhesion pigment granule maturation regulation of vascular endothelial growth factor signaling pathway regulation of rubidium ion transport positive regulation of integrin-mediated signaling pathway
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us
          • Careers
          • Investors
          • News
          • Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy
          © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-7bf687f5d8-dsxhx:80/100.66.134.36:80.
          git-commit: 7fd278a50357815940906fb4686aaa1c76cc7991
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.30.1-2025.05.22-1.0