Invitrogen

CD63 Monoclonal Antibody (H5C6), eFluor™ 450, eBioscience™

8 Published Figures

10 References

View all (140) CD63 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

FIGURE: 1 / 9

CD63 Antibody (48-0639-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human IgE FITC (Product # 11-6986-42), Anti-Human CD193 (CCR3) PE (Product # 12-1939-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (blue histogram) or Anti-Human CD63 eFluor® 450 (purple histogram). CD193+IgG+ cells (left) were used for analysis.

Product Image Gallery

Product Details

48-0639-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (1 µg)/test

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

H5C6

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2574023

Product Specific Information

Description: This H5C6 monoclonal antibody reacts with human CD63, a type III member of the tetraspanin family of transmembrane proteins. CD63 is expressed intracellularly on lysosomes, endosomes, and granules of resting platelets and basophils. However, cell surface expression of CD63 can be detected on activated basophils and platelets, monocytes, macrophages, and granulocytes. This receptor is also expressed on endothelial cells, fibroblasts, and smooth muscle cells. Studies have demonstrated that CD63 associates with integrins (VLA-3 and VLA-6) and TIMP-1 to mediate the allergic response.

Applications Reported: This H5C6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H5C6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD63, also known as LAMP-3, is a glycoprotein belonging to the tetraspanin family, primarily located in late endosomes, lysosomes, and secretory vesicles across various cell types. It is expressed intracellularly in resting platelets and basophils, but upon activation, CD63 translocates to the plasma membrane, making it a widely used marker for basophil activation. Unlike basophils, mast cells can expose CD63 without activation. CD63 is expressed on activated platelets, monocytes, macrophages, and granulocytes, as well as endothelial cells, fibroblasts, and smooth muscle cells. It interacts with integrins (such as VLA-3 and VLA-6) and TIMP-1, playing roles in mediating allergic responses, phagocytosis, and cell migration. Additionally, CD63 is involved in the regulation of H/K-ATPase trafficking and ROMK1 channels, and serves as a T-cell costimulation molecule. CD63 expression can be used to predict prognosis in early-stage carcinomas and is identical to the melanoma-associated antigen ME491 and the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome, highlighting its significance in both immune responses and disease processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Neuroprotective Effects of Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cell Extracellular Vesicles in Ischemic Stroke Models.

Biomedicines

Lu G,Su X,Wang L,Leung CK,Zhou J,Xiong Z,Wang W,Liu H,Chan WY

Published figure using CD63 monoclonal antibody (Product # 48-0639-42) in Flow Cytometry

Sun Sep 17 00:00:00 UTC 2023

Stem cell-derived exosomes from human exfoliated deciduous teeth promote angiogenesis in hyperglycemic-induced human umbilical vein endothelial cells.

Journal of applied oral science : revista FOB

Sunartvanichkul T,Arayapisit T,Sangkhamanee SS,Chaweewannakorn C,Iwasaki K,Klaihmon P,Sritanaudomchai H

Published figure using CD63 monoclonal antibody (Product # 48-0639-42) in Flow Cytometry

Fri Apr 14 00:00:00 UTC 2023

M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties.

Cell death discovery

Zhao G,Ding L,Yu H,Wang W,Wang H,Hu Y,Qin L,Deng G,Xie B,Li G,Qi L

Published figure using CD63 monoclonal antibody (Product # 48-0639-42) in Flow Cytometry

Thu Aug 04 00:00:00 UTC 2022

Cutting edge approaches for rapid characterization of airway exosomes.

Methods (San Diego, Calif.)

Hough KP,Deshane JS

48-0639 was used in Flow cytometry/Cell sorting to describe two methods of rapid exosomes characterization: (1) imaging flow cytometry using ImageStream; and (2) conventional flow cytometry using the BD Symphony A5 platform.

Fri May 01 00:00:00 UTC 2020

Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells.

Journal of ovarian research

Cheng L,Zhang K,Qing Y,Li D,Cui M,Jin P,Xu T

Published figure using CD63 monoclonal antibody (Product # 48-0639-42) in Flow Cytometry

Wed Jan 22 00:00:00 UTC 2020

Ionizing Radiation Increases the Activity of Exosomal Secretory Pathway in MCF-7 Human Breast Cancer Cells: A Possible Way to Communicate Resistance against Radiotherapy.

International journal of molecular sciences

Jabbari N,Nawaz M,Rezaie J

Published figure using CD63 monoclonal antibody (Product # 48-0639-42) in Flow Cytometry

Thu Jul 25 00:00:00 UTC 2019

Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics.

Scientific reports

Hough KP,Wilson LS,Trevor JL,Strenkowski JG,Maina N,Kim YI,Spell ML,Wang Y,Chanda D,Dager JR,Sharma NS,Curtiss M,Antony VB,Dransfield MT,Chaplin DD,Steele C,Barnes S,Duncan SR,Prasain JK,Thannickal VJ,Deshane JS

48-0639 was used in Flow cytometry/Cell sorting to characterise the lipid composition of extracellular vesicless from asthmatics and healthy subjects, as well as secondhand smoke (SHS) exposed subjects from each group.

Mon Jul 09 00:00:00 UTC 2018

MicroRNA-containing extracellular vesicles released from endothelial colony-forming cells modulate angiogenesis during ischaemic retinopathy.

Journal of cellular and molecular medicine

Dellett M,Brown ED,Guduric-Fuchs J,O'Connor A,Stitt AW,Medina RJ,Simpson DA

Published figure using CD63 monoclonal antibody (Product # 48-0639-42) in Flow Cytometry

Fri Dec 01 00:00:00 UTC 2017

Urinary exosomes as a novel biomarker for evaluation of α-lipoic acid's protective effect in early diabetic nephropathy.

Journal of clinical laboratory analysis

Sun H,Yao W,Tang Y,Zhuang W,Wu D,Huang S,Sheng H

Published figure using CD63 monoclonal antibody (Product # 48-0639-42) in Flow Cytometry

Wed Nov 01 00:00:00 UTC 2017

Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.

PloS one

Wu S,Ju GQ,Du T,Zhu YJ,Liu GH

Published figure using CD63 monoclonal antibody (Product # 48-0639-42) in Flow Cytometry

Tue Oct 22 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: CD 63; CD63; CD63 antigen; CD63 antigen (melanoma 1 antigen); Granulophysin; LAMP-3; Limp1; Lysosomal-associated membrane protein 3; Lysosome integral membrane protein 1; lysosome-associated membrane glycoprotein 3; Melanoma-associated antigen ME491; melanoma-associated antigen MLA1; Ocular melanoma-associated antigen; OMA81H; Tetraspanin-30; Tspan-30

View more View less

Gene Aliases: CD63; LAMP-3; ME491; MLA1; OMA81H; TSPAN30

View more View less

UniProt ID: (Human) P08962

View more View less

Entrez Gene ID: (Human) 967

View more View less

Function(s)

protein binding protein complex binding

Process(es)

positive regulation of receptor internalization platelet degranulation cell-matrix adhesion negative regulation of epithelial cell migration protein transport cell migration epithelial cell differentiation cellular protein localization endosome to melanosome transport positive regulation of cell adhesion pigment granule maturation regulation of vascular endothelial growth factor signaling pathway regulation of rubidium ion transport positive regulation of integrin-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD63 Monoclonal Antibody (H5C6), eFluor™ 450, eBioscience™

CD63 Antibody (48-0639-42) in Flow

Product Details

48-0639-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!