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Invitrogen
CD63 Monoclonal Antibody (NVG-2), PE-Cyanine7, eBioscience™
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FIGURE: 1 / 2
CD63 Antibody (25-0631-82) in Flow
Intracellular staining of mouse resident peritoneal exudate cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.06 µg of Anti-Mouse CD63 PE-Cyanine7 (right) using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Total viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.
Product Details
25-0631-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
NVG-2
Conjugate
Excitation/Emission Max
569/780 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573356
Product Specific Information
Description: The monoclonal antibody NVG-2 reacts with mouse CD63, also known as Lysosomal-Associated Membrane Protein 3 (LAMP-3) or tetraspanin 30 (TSPN30), a member of tetraspanin family of proteins characterized by four transmembrane domains. CD63 is expressed on a variety of cell types of hematopoietic lineage, e.g., granulocytes, B lymphocytes, platelets, as well as cells of non-hematopoietic origin. It can be found on the cell membrane, late endocytic vesicles, lysosomes, exosomes, and other specialized granules. On the cell surface, CD63 has been shown to interact with various proteins forming tetraspanin-enriched microdomains (TEM). Its high expression on the cell membrane may be indicative of cell activation, hence, CD63 is often used as an activation marker for basophils, platelets and other cells.
Applications Reported: This NVG-2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This NVG-2 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse resident peritoneal exudate cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. CD63 is also present in the plasma membrane, usually following cell activation. Hence, CD63 has become a widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas. CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40. Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD 63; CD63; CD63 antigen; melanoma 1 antigen
Gene Aliases: C75951; Cd63; ME491; Tspan30
UniProt ID: (Mouse) P41731
Entrez Gene ID: (Mouse) 12512
positive regulation of receptor internalization
transport
cell-matrix adhesion
negative regulation of epithelial cell migration
protein transport
cell migration
epithelial cell differentiation
cellular protein localization
endosome to melanosome transport
pigmentation
positive regulation of cell adhesion
positive regulation of endocytosis
pigment granule maturation
pigment cell differentiation
regulation of vascular endothelial growth factor signaling pathway
regulation of rubidium ion transport
positive regulation of integrin-mediated signaling pathway
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