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Invitrogen
CD64 Monoclonal Antibody (10.1), PE-eFluor™ 610, eBioscience™
FIGURE: 1 / 4
CD64 Antibody (61-0649-42) in Flow
Normal human peripheral blood cells were stained with CD14 Monoclonal Antibody, FITC (Product # 11-0149-42) and Mouse IgG1 kappa Isotype Control, PE-eFluor 610 (Product # 61-4714-82) (left) or CD64 Monoclonal Antibody, PE-eFluor 610 (right). Cells in the monocyte gate were used for analysis.
Product Details
61-0649-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
10.1
Conjugate
Excitation/Emission Max
565/606 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2744760
Product Specific Information
Description: The 10.1 monoclonal antibody reacts with human CD64 (FcRI), a 75 kDa type I transmembrane protein. CD64 is the high affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and regulation of cytokine production. Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative.
Applications Reported: The 10.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 10.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Target Information
CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD64; CD64 antigen; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc gamma receptor; Fc gamma receptor Ia; Fc-gamma receptor 1; Fc-gamma receptor I A1; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FcgRI; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; RP11-196G18.2
Gene Aliases: CD64; CD64A; FCG1; FCGR1; FCGR1A; FCRI; IGFR1
UniProt ID: (Human) P12314
Entrez Gene ID: (Human) 2209
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
receptor-mediated endocytosis
phagocytosis, engulfment
immune response
signal transduction
Fc-gamma receptor signaling pathway involved in phagocytosis
regulation of immune response
interferon-gamma-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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