Invitrogen

CD66 Monoclonal Antibody (CD66a-B1.1), APC, eBioscience™

FIGURE: 1 / 2

CD66 Antibody (17-0668-41) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD16 FITC (Product # 11-0168-42) and Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or Anti-Human CD66 (right). Cells in the granulocyte gate were used for analysis.

Product Image Gallery

Product Details

17-0668-41

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CD66a-B1.1

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

AB_11043404

Product Specific Information

Description: The CD66a-B1.1 monoclonal antibody reacts with human CD66a, c, d, and e, which are also known as CEACAM1, CEACAM6, CEACAM3 and CEA, respectively. CD66 is expressed on endothelial and epithelial cells, as well as on neutrophils and monocytes. Myeloma and cancer cells also express CD66. Moreover, expression of this receptor can be induced on T cells, B cells, and CD16-negative NK cells. CD66 exists as multiple splice variants that differ in the length of their cytoplasmic domain, which contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that mediates the inhibitory functions of this cell surface protein on T and NK cells. CD66 is involved in cell adhesion and growth, neutrophil activation, and signaling. Finally, studies have shown that the CD66 variants interact with each other homo- and heterotypically.

Applications Reported: This CD66a-B1.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CD66a-B1.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD66 molecules are 180-200 kDa glycoproteins of carcinoembryonic antigen family. They are present on all blood granulocytes and some tissue macrophages, but are absent from other hematopoietic cells. The expression of CD66 increases significantly on granulocytes upon their activation. CD66a (BGP1, CEACAM1) and CD66d (CGM1, CEACAM3), as well as CEACAM2 and 4, are transmembrane proteins, whereas CD66c (CEAL, NCA, CEACAM6) and CD66e (CEA, CEACAM5), as well as CEACAM7 and 8, are anchored to the plasma membrane by C-terminal glycosylphosphatidylinositol (GPI) lipid moiety.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Alteration of immune profiles is associated with pulmonary function and symptoms in patients with chronic obstructive pulmonary disease.

Molecular medicine reports

Li S,Zhao S,Wu Z,Wang F,Li W

17-0668-42 was used in Flow cytometry/Cell sorting to determine the expression profiles of immune cells and inflammatory factors of patients with COPD and healthy controls, and to analyze the relationship between immune profiles and smoking history.

Mon Nov 01 00:00:00 UTC 2021