Invitrogen

CD68 Polyclonal Antibody

4 Published Figures

10 References

View all (176) CD68 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (18)
Published Figures (4)

CD68 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 22

CD68 Antibody (PA5-78996) in IHC (P)

Immunohistochemistry (Paraffin) analysis of CD68 in paraffin-embedded section of mouse spleen tissue using CD68 Polyclonal Antibody (Product # PA5-78996). Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with the primary antibody at a 5 µg/mL dilution overnight at 4°C. ... View More

CD68 Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD68 Antibody in Immunohistochemistry (IHC)

CD68 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))

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Product Details

PA5-78996

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

1 µg/mL

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of mouse CD68 (312-326aa AFCITRRRQSTYQPL).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2746112

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: rat spleen tissue, mouse spleen tissue, mouse RAW2647 whole cell. IHC: mouse liver tissue, mouse spleen tissue, rat spleen tissue, rat lung tissue, rat lung tissue, rat liver tissue. Flow: RAW2647 cell.

Target Information

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fibroblast Activation Protein Activates Macrophages and Promotes Parenchymal Liver Inflammation and Fibrosis.

Cellular and molecular gastroenterology and hepatology

Yang AT,Kim YO,Yan XZ,Abe H,Aslam M,Park KS,Zhao XY,Jia JD,Klein T,You H,Schuppan D

PA5-78996 was used in Immunohistochemistry to show that pharmacological fibroblast activation protein (FAP) inhibition attenuates inflammation-predominant liver fibrosis. FAP is expressed on subsets of activated fibroblasts/hepatic stellate cells (HSC) and promotes both macrophage and HSC profibrogenic activity in liver fibrosis.

Tue Mar 21 00:00:00 UTC 2023

Fibroblast Activation Protein Activates Macrophages and Promotes Parenchymal Liver Inflammation and Fibrosis.

Cellular and molecular gastroenterology and hepatology

Yang AT,Kim YO,Yan XZ,Abe H,Aslam M,Park KS,Zhao XY,Jia JD,Klein T,You H,Schuppan D

Tue Mar 21 00:00:00 UTC 2023

Intestinal Flora Composition Determines Microglia Activation and Improves Epileptic Episode Progress.

Frontiers in cellular and infection microbiology

Ding X,Zhou J,Zhao L,Chen M,Wang S,Zhang M,Zhang X,Jiang G

Published figure using CD68 polyclonal antibody (Product # PA5-78996) in Western Blot

Fri Apr 01 00:00:00 UTC 2022

Tumor cell-based vaccine contributes to local tumor irradiation by eliciting a tumor model-dependent systemic immune response.

Frontiers in immunology

Remic T,Sersa G,Levpuscek K,Lampreht Tratar U,Ursic Valentinuzzi K,Cör A,Kamensek U

PA5-78996 was used in Immunohistochemistry to demonstrate that vaccine elicits an immune response and contributes to the antitumor efficacy of tumor irradiation; this interaction is multi-faceted and appears to be dependent on the tumor immunogenicity.

Fri Sep 23 00:00:00 UTC 2022

Construction of Exosomes that Overexpress CD47 and Evaluation of Their Immune Escape.

Frontiers in bioengineering and biotechnology

Ben XY,Wang YR,Zheng HH,Li DX,Ren R,Ni PL,Zhang HY,Feng RJ,Li YQ,Li QF,Yi XN

PA5-78996 was used in Western Blotting to show that macrophages ingested significantly more control EXOs than EXOsCD47 (p 0.01), with confirmation by ultra-high-definition laser confocal microscopy.

Tue Jul 19 00:00:00 UTC 2022

Nicotine Exacerbates TAAD Formation Induced by Smooth Muscle-Specific Deletion of the TGF-β Receptor 2.

Journal of immunology research

Chun C,Qi X,Wang F,Madrid KB,Saldarriaga LA,Fisch MR,Brantly ML,Upchurch GR,Jiang Z

PA5-78996 was used in Immunohistochemistry (Paraffin) to develop a mouse model that may be utilized to investigate exacerbation of thoracic aortic aneurysms and dissections formation by mimetics of tobacco smoke.

Mon Feb 07 00:00:00 UTC 2022

Circulating Pro-Inflammatory Exosomes Worsen Stroke Outcomes in Aging.

Circulation research

Zhang H,Lin S,McElroy CL,Wang B,Jin D,Uteshev VV,Jin K

PA5-78996 was used in Immunohistochemistry to test the hypothesis that peripheral circulating exosomes derived from young or aged animals may serve as vehicles to deliver beneficial or detrimental signals, respectively, from the periphery to the brain and may thus influence functional outcomes after ischemic stroke.

Fri Sep 17 00:00:00 UTC 2021

One-Step Large-Scale Nanotexturing of Nonplanar PTFE Surfaces to Induce Bactericidal and Anti-inflammatory Properties.

ACS applied materials & interfaces

Xu J,Moon H,Xu J,Lim J,Fischer T,McNally HA,Sintim HO,Lee H

PA5-78996 was used in Immunohistochemistry (Paraffin) to to demonstrate a simple and scalable nanotexturing method for both planar (films) and nonplanar (tubes) polytetrafluoroethylene (PTFE) surfaces using a commercial desktop oxygen plasma etcher.

Wed Jun 17 00:00:00 UTC 2020

A validated mouse model capable of recapitulating the protective effects of female sex hormones on ascending aortic aneurysms and dissections (AADs).

Physiological reports

Qi X,Wang F,Chun C,Saldarriaga L,Jiang Z,Pruitt EY,Arnaoutakis GJ,Upchurch GR,Jiang Z

Published figure using CD68 polyclonal antibody (Product # PA5-78996) in Immunohistochemistry

Sun Nov 01 00:00:00 UTC 2020

A validated mouse model capable of recapitulating the protective effects of female sex hormones on ascending aortic aneurysms and dissections (AADs).

Physiological reports

Qi X,Wang F,Chun C,Saldarriaga L,Jiang Z,Pruitt EY,Arnaoutakis GJ,Upchurch GR,Jiang Z

Published figure using CD68 polyclonal antibody (Product # PA5-78996) in Immunohistochemistry

Sun Nov 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: CD68; CD68 antigen; Macrosialin

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Gene Aliases: Cd68; gp110; Lamp4; Scard1

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UniProt ID: (Mouse) P31996

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Entrez Gene ID: (Mouse) 12514, (Rat) 287435

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Process(es)

cellular response to organic substance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD68 Polyclonal Antibody

CD68 Antibody (PA5-78996) in IHC (P)

Product Details

PA5-78996

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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