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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD68 Antibodies

          Invitrogen

          CD68 Monoclonal Antibody (FA-11), eBioscience™

          Advanced Verification
          17 Published Figures
          36 References
          View all (176) CD68 antibodies

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          Datasheet
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          Datasheet
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          Cite CD68 Monoclonal Antibody (FA-11), eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (17)
          • Advanced Verification (1)
          CD68 Antibody in Immunohistochemistry (Frozen) (IHC (F))
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          CD68 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          Group 53 Created with Sketch.

          FIGURE: 1 / 19

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          CD68 Antibody (14-0681-82) in IHC (F)

          Immunohistochemistry of frozen mouse spleen using 5 µg/mL of Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (left) or 5 µg/mL of Anti-Mouse CD68 Purified (right) followed by 5 µg/mL of Anti-Rat IgG TRITC. Nuclei are stained with DAPI. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD68 Antibody in Immunohistochemistry (Frozen) (IHC (F))
          CD68 Antibody in Immunohistochemistry (IHC)
          CD68 Antibody in Immunocytochemistry (ICC/IF)
          CD68 Antibody in Immunocytochemistry (ICC/IF)
          CD68 Antibody in Immunocytochemistry (ICC/IF)
          CD68 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD68 Antibody in Immunohistochemistry (IHC)
          CD68 Antibody in Immunohistochemistry (IHC)
          CD68 Antibody in Immunohistochemistry (IHC)
          CD68 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
          CD68 Antibody in Immunohistochemistry (IHC)
          CD68 Antibody in Immunohistochemistry (IHC)
          CD68 Antibody in Immunohistochemistry (IHC)
          CD68 Antibody in Immunohistochemistry (IHC)
          CD68 Antibody in Immunohistochemistry (IHC)
          CD68 Antibody in Immunohistochemistry (IHC)
          CD68 Antibody in Western Blot (WB)
          CD68 Antibody in Flow Cytometry (Flow)
          CD68 Antibody

          Product Details

          14-0681-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 24 publications 24 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 1 publication 1 publication

          Immunohistochemistry (Frozen) (IHC (F))

          5 µg/mL
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          View 4 publications 4 publications

          Flow Cytometry (Flow)

          Assay-Dependent
          View 4 publications 4 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rat / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          FA-11

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • APC-eFluor 780
          • Brilliant Violet 421
          • Brilliant Violet 786
          • eFluor 660
          • PE 
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-eFluor 710
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2572857

          Product Specific Information

          Description: This FA-11 monoclonal antibody reacts with mouse CD68, known also as macrosialin. CD68 is a heavily O- and N- glycosylated type I integral membrane protein belonging to the sialomucin family and is closely related to the Lysosomal/endosomal-Associated Membrane Glycoproteins (LAMP). Although commonly used as a pan-macrophage marker, low levels of expression have also been observed in Langerhans cells and conventional dendritic cells. CD68 is mostly localized in the late endosomes and lysosomes with a smaller fraction circulating to the cell surface. In the steady state, only about 10-15% of CD68 is present at the surface. Cell stimulation increases expression of CD68, its transport to cell surface, and results in glycoform remodeling. CD68 binds oxidized LDL and is involved in the process of phagocytosis.

          The FA-11 antibody is not recommended for use in immunohistochemistry of formalin-fixed paraffin embedded tissues.

          Applications Reported: This FA-11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. (Fluorochrome conjugated FA-11 is recommended for use in intracellular flow cytometry.).

          Applications Tested: Anti-Mouse CD68 Purified has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD68; Macrosialin

          View more View less

          Gene Aliases: Cd68; gp110; Lamp4; Scard1

          View more View less

          UniProt ID: (Mouse) P31996

          View more View less

          Entrez Gene ID: (Mouse) 12514

          View more View less

          Process(es)
          cellular response to organic substance
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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