Invitrogen

CD68 Monoclonal Antibody (FA-11), FITC

3 Published Figures

11 References

View all (176) CD68 antibodies

Datasheet

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Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (3)

FIGURE: 1 / 3

CD68 Antibody (MA5-16676) in Flow

Journal of applied microbiology 2022 - 5 FIGURE L-norvaline combined with DOX affects the proliferation of breast cancer cells. (a) Identification of CD68 + CD206 positive cells by flow cytometry. (b) The gene expression of Arg-1 and IL-10 by quantitative real-time PCR . (c) The proliferative activity of cells after treatment with different concentrations of L-norvaline. (d) The proliferative activity of cells after treatment with DOX and differ... View More

CD68 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Product Details

MA5-16676

Applications

Tested Dilution

Publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 9 publications 9 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

FA-11

Immunogen

purified Con A acceptor glycoprotein from P815 cell line

View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2538170

Product Specific Information

Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Rat anti Mouse CD68 antibody, clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.

Rat anti Mouse CD68 antibody, clone FA-11 recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, which is classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.

Target Information

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The polarization of microglia and infiltrated macrophages in the injured mice spinal cords: a dynamic analysis.

PeerJ

Li JL,Fu GQ,Wang YY,Bian MM,Xu YM,Zhang L,Chen YQ,Zhang N,Ding SQ,Wang R,Fang R,Tang J,Hu JG,Lü HZ

MA5-16676 was used in Flow cytometry/Cell sorting to study if M1 cells in injured spinal cords were mainly derived from MG rather than infiltrating Mø.

Tue Feb 28 00:00:00 UTC 2023

Dendritic cell-mediated chronic low-grade inflammation is regulated by the RAGE-TLR4-PKCβ₁ signaling pathway in diabetic atherosclerosis.

Molecular medicine (Cambridge, Mass.)

Zhao L,Li Y,Xu T,Lv Q,Bi X,Liu X,Fu G,Zou Y,Ge J,Chen Z,Zhang W

MA5-16676 was used in Immunohistochemistry (PFA fixed) to demonstrate that activation of the RAGE-TLR4-PKC1 signal pathway in a high-glucose and high-fat environment was involved in the maturation of dendritic cells and development of chronic inflammation.

Fri Jan 21 00:00:00 UTC 2022

Dendritic cell-mediated chronic low-grade inflammation is regulated by the RAGE-TLR4-PKCβ₁ signaling pathway in diabetic atherosclerosis.

Molecular medicine (Cambridge, Mass.)

Zhao L,Li Y,Xu T,Lv Q,Bi X,Liu X,Fu G,Zou Y,Ge J,Chen Z,Zhang W

Fri Jan 21 00:00:00 UTC 2022

L-norvaline affects the proliferation of breast cancer cells based on the microbiome and metabolome analysis.

Journal of applied microbiology

Zhu Q,Zai H,Zhang K,Zhang X,Luo N,Li X,Hu Y,Wu Y

Published figure using CD68 monoclonal antibody (Product # MA5-16676) in Flow Cytometry

Mon Aug 01 00:00:00 UTC 2022

The alveolar macrophage toponome of female SP-A knockout mice differs from that of males before and after SP-A1 rescue.

Scientific reports

Phelps DS,Chinchilli VM,Yang L,Shearer D,Weisz J,Zhang X,Floros J

MA5-16676 was used in flow cytometry to study the expression of alveolar macrophages in male and female mice lacking surfactant protein A.

Wed Mar 23 00:00:00 UTC 2022

VX-765 reduces neuroinflammation after spinal cord injury in mice.

Neural regeneration research

Chen J,Chen YQ,Shi YJ,Ding SQ,Shen L,Wang R,Wang QY,Zha C,Ding H,Hu JG,Lü HZ

MA5-16676 was used in Flow Cytometry to suggest that VX-765 can reduce neuroinflammation and improve nerve function recovery after spinal cord injury by inhibiting caspase-1/interleukin-1β/interleukin-18.

Wed Sep 01 00:00:00 UTC 2021

Single-Cell Transcriptome Profiles Reveal Fibrocytes as Potential Targets of Cell Therapies for Abdominal Aortic Aneurysm.

Frontiers in cardiovascular medicine

Li B,Song X,Guo W,Hou Y,Hu H,Ge W,Fan T,Han Z,Li Z,Yang P,Gao R,Zhao H,Wang J

MA1-82739 was used in Flow Cytometry to determine the roles of different vascular and immune cells in abdominal aortic aneurysm formation and pathogenesis.

Wed Dec 15 00:00:00 UTC 2021

COX2 regulates senescence secretome composition and senescence surveillance through PGE₂.

Cell reports

Gonçalves S,Yin K,Ito Y,Chan A,Olan I,Gough S,Cassidy L,Serrao E,Smith S,Young A,Narita M,Hoare M

MA5-16676 was used in Flow Cytometry to show that, during RAS-induced senescence, COX2 is a critical regulator of the inflammatory senescence-associated secretory phenotype composition and senescence surveillance in vivo.

Tue Mar 16 00:00:00 UTC 2021

COX2 regulates senescence secretome composition and senescence surveillance through PGE₂.

Cell reports

Gonçalves S,Yin K,Ito Y,Chan A,Olan I,Gough S,Cassidy L,Serrao E,Smith S,Young A,Narita M,Hoare M

Tue Mar 16 00:00:00 UTC 2021

CRID3, a blocker of apoptosis associated speck like protein containing a card, ameliorates murine spinal cord injury by improving local immune microenvironment.

Journal of neuroinflammation

Chen YQ,Wang SN,Shi YJ,Chen J,Ding SQ,Tang J,Shen L,Wang R,Ding H,Hu JG,Lü HZ

MA5-16676 was used in Flow cytometry/Cell sorting to study the effect of CRID3 on the local microenvironment and the possible role in neuroprotection following spinal cord injury.

Sat Aug 29 00:00:00 UTC 2020

Model-Based Characterization of Inflammatory Gene Expression Patterns of Activated Macrophages.

PLoS computational biology

Rex J,Albrecht U,Ehlting C,Thomas M,Zanger UM,Sawodny O,Häussinger D,Ederer M,Feuer R,Bode JG

MA182739 was used in flow cytometry to model the expression patterns of chemokines and cytokines that turn into M1/M2 macrophage activation.

Fri Jul 01 00:00:00 UTC 2016

Intravenous infusion of monocytes isolated from 2-week-old mice enhances clearance of Beta-amyloid plaques in an Alzheimer mouse model.

PloS one

Hohsfield LA,Humpel C

MA1-82739 was used in flow cytometry to assess the effect of CD11b-positive (+) monocytes on Alzheimer's disease using a mouse model

Tue Apr 05 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: CD68; Macrosialin

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Gene Aliases: Cd68; gp110; Lamp4; Scard1

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UniProt ID: (Mouse) P31996

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Entrez Gene ID: (Mouse) 12514

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Process(es)

cellular response to organic substance

Performance Guarantee

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CD68 Monoclonal Antibody (FA-11), FITC

CD68 Antibody (MA5-16676) in Flow

Product Details

MA5-16676

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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