Invitrogen

CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), eBioscience™

CD68 Antibody in Immunocytochemistry (ICC/IF)

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CD68 Antibody (14-0689-82) in ICC/IF

Immunofluorescence analysis of Cd68 was performed using 70% confluent log phase K-562 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), eBioscience (Product # 14-0689-82) at a concentration of 1 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary ... View More

CD68 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD68 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD68 Antibody in Immunohistochemistry (IHC)

CD68 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

Product Image Gallery

CD68 Antibody — Antibody clone (eBioY1/82A (Y1/82A)) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in eBioY1/82A (Y1/82A) KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD68 antibody (eBioY1/82A (Y1/82A)). Yellow histogram represents staining with the isotype control. {KO}

Product Details

14-0689-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 13 publications 13 publications

Immunomicroscopy (IM)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

eBioY1/82A (Y1/82A)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_795874

Product Specific Information

Description: The eBioY1/82 antibody reacts with CD68 which belongs to the sialomucin family and is closely related to the family of lysosomal-associated membrane proteins (lamps) and scavenger receptor. CD68 is predominantly an intracellular protein, found mainly in the late endosomal compartment but can be detected in smaller amounts on the surface of cells of mainly myeloid derived cells; monocytes and macrophages, dendritic cells (and in some langerhan cells), neutrophils, basophils mast cells, myeloid progenitor cells, and a subset of CD34+ hemopoietic bone marrow progenitor cells. The function has not been fully elucidated but based on it's homolgy and structure suggests a role in antigen processing or presentation and protection of the lysosomal membrane from hydrolytic enzymes. Reports have also seen expression in activated T cells, and about 40% of peripheral blood B-lymphocytes and 50% of all B-ALL.

Staining with eBioY1/82A shows surface staining on B cells and a minor percentage of monocytes while intracellular staining results in about 50% of B cells and most monocytes.

Applications Reported: This eBioY1/82A (Y1/82A) antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.

Applications Tested: This eBioY1/82A (Y1/82A) antibody has been tested by intracellular staining and flow cytometric analysis. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Activation of the JNK/COX-2/HIF-1α axis promotes M1 macrophage via glycolytic shift in HIV-1 infection.

Life science alliance

Zhang J,Yuan Z,Li X,Wang F,Wei X,Kang Y,Mo C,Jiang J,Liang H,Ye L

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Flow Cytometry

Fri Dec 01 00:00:00 UTC 2023

Complete Freund's Adjuvant Induces a Fibroblast-like Synoviocytes (FLS) Metabolic and Migratory Phenotype in Resident Fibroblasts of the Inoculated Footpad at the Earliest Stage of Adjuvant-Induced Arthritis.

Cells

González-Chávez SA,Chaparro-Barrera E,Alvarado-Jáquez MF,Cuevas-Martínez R,Ochoa-Albíztegui RE,Pacheco-Tena C

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Immunohistochemistry

Wed Mar 08 00:00:00 UTC 2023

Targeting Glioblastoma-Associated Macrophages for Photodynamic Therapy Using AGuIX^®-Design Nanoparticles.

Pharmaceutics

Lerouge L,Gries M,Chateau A,Daouk J,Lux F,Rocchi P,Cedervall J,Olsson AK,Tillement O,Frochot C,Acherar S,Thomas N,Barberi-Heyob M

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Immunohistochemistry

Mon Mar 20 00:00:00 UTC 2023

PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.

CNS neuroscience & therapeutics

Zhang H,Wang Y,Zhao Y,Liu T,Wang Z,Zhang N,Dai Z,Wu W,Cao H,Feng S,Zhang L,Cheng Q,Liu Z

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Flow Cytometry

Tue Nov 01 00:00:00 UTC 2022

LINC00240/miR-155 axis regulates function of trophoblasts and M2 macrophage polarization via modulating oxidative stress-induced pyroptosis in preeclampsia.

Molecular medicine (Cambridge, Mass.)

Wu HY,Liu K,Zhang JL

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Flow Cytometry

Sat Sep 24 00:00:00 UTC 2022

Divide and Conquer: Isolating Cell Populations to Investigate How Breast Cancer Risk Factors Alter the Breast Microenvironment.

Methods in molecular biology (Clifton, N.J.)

Arendt LM

14-0689-82 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen) to investigate how risk factors impact distinct cell populations within breast tissue.

Mon Feb 21 00:00:00 UTC 2022

Different Induction of PD-L1 (CD274) and PD-1 (CD279) Expression in THP-1-Differentiated Types 1 and 2 Macrophages.

Journal of inflammation research

Lai CY,Tseng PC,Chen CL,Satria RD,Wang YT,Lin CF

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Flow Cytometry

Wed Apr 27 00:00:00 UTC 2022

Decreased ferroportin in hepatocytes promotes macrophages polarize towards an M2-like phenotype and liver fibrosis.

Scientific reports

Cai C,Zeng D,Gao Q,Ma L,Zeng B,Zhou Y,Wang H

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Immunohistochemistry (Paraffin)

Mon Jun 28 00:00:00 UTC 2021

Antitumor properties of triptolide: phenotype regulation of macrophage differentiation.

Cancer biology & therapy

Li H,Li L,Mei H,Pan G,Wang X,Huang X,Wang T,Jiang Z,Zhang L,Sun L

14-0689-82 was used in Flow Cytometry to determine whether triptolide has any effect on the activation of tumor-associated macrophages and the production of tumor-promoting mediators.

Mon Mar 29 00:00:00 UTC 2021

The role of CD68+ macrophage in classical Hodgkin lymphoma patients from Egypt.

Diagnostic pathology

Mohamed O,El Bastawisy A,Allahlobi N,Abdellateif MS,Zekri ARN,Shaarawy S,Korany Z,Mohanad M,Bahnassy AA

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Immunohistochemistry

Tue Feb 04 00:00:00 UTC 2020

The role of CD68+ macrophage in classical Hodgkin lymphoma patients from Egypt.

Diagnostic pathology

Mohamed O,El Bastawisy A,Allahlobi N,Abdellateif MS,Zekri ARN,Shaarawy S,Korany Z,Mohanad M,Bahnassy AA

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Immunohistochemistry

Tue Feb 04 00:00:00 UTC 2020

Nanotopographical Coatings Induce an Early Phenotype-Specific Response of Primary Material-Resident M1 and M2 Macrophages.

Materials (Basel, Switzerland)

Schmitz T,Jannasch M,Weigel T,Moseke C,Gbureck U,Groll J,Walles H,Hansmann J

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Immunomicroscopy

Wed Mar 04 00:00:00 UTC 2020

An altered response in macrophage phenotype following damage in aged human skeletal muscle: implications for skeletal muscle repair.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Sorensen JR,Kaluhiokalani JP,Hafen PS,Deyhle MR,Parcell AC,Hyldahl RD

14-0689 was used in Immunohistochemistry to test the hypothesis that macrophage polarisation is altered in old compared to young skeletal muscle, possibly contributing to the poor satellite cell response observed in older muscle tissue.

Sun Sep 01 00:00:00 UTC 2019

Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis.

Science advances

Izadi D,Layton TB,Williams L,McCann F,Cabrita M,Espirito Santo AI,Xie W,Fritzsche M,Colin-York H,Feldmann M,Midwood KS,Nanchahal J

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Flow Cytometry

Sun Dec 01 00:00:00 UTC 2019

Macrophagic CD146 promotes foam cell formation and retention during atherosclerosis.

Cell research

Luo Y,Duan H,Qian Y,Feng L,Wu Z,Wang F,Feng J,Yang D,Qin Z,Yan X

14-0689 was used in Immunofluorescence to identify CD146 as a retention signal that traps macrophages within the artery wall, and a promising therapeutic target in atherosclerosis treatment.

Wed Mar 01 00:00:00 UTC 2017

Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.

Journal of neurovirology

Garcia-Mesa Y,Jay TR,Checkley MA,Luttge B,Dobrowolski C,Valadkhan S,Landreth GE,Karn J,Alvarez-Carbonell D

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2017

Suppression of the inflammatory response by disease-inducible interleukin-10 gene therapy in a three-dimensional micromass model of the human synovial membrane.

Arthritis research & therapy

Broeren MG,de Vries M,Bennink MB,Arntz OJ,van Lent PL,van der Kraan PM,van den Berg WB,van den Hoogen FH,Koenders MI,van de Loo FA

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Immunohistochemistry

Fri Aug 12 00:00:00 UTC 2016

MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.

Bioscience reports

Zhong Y,Yi C

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Flow Cytometry

Mon Aug 01 00:00:00 UTC 2016

Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.

Scientific reports

Tsai CS,Lin YW,Huang CY,Shih CM,Tsai YT,Tsao NW,Lin CS,Shih CC,Jeng H,Lin FY

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Immunocytochemistry

Fri Dec 02 00:00:00 UTC 2016

Thrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.

Scientific reports

Tsai CS,Lin YW,Huang CY,Shih CM,Tsai YT,Tsao NW,Lin CS,Shih CC,Jeng H,Lin FY

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Immunocytochemistry

Fri Dec 02 00:00:00 UTC 2016

Expression of Lectin-Like Transcript 1, the Ligand for CD161, in Rheumatoid Arthritis.

PloS one

Chalan P,Bijzet J,Huitema MG,Kroesen BJ,Brouwer E,Boots AM

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Immunohistochemistry

Mon Apr 11 00:00:00 UTC 2016

Doxycycline inhibits polarization of macrophages to the proangiogenic M2-type and subsequent neovascularization.

The Journal of biological chemistry

He L,Marneros AG

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Flow Cytometry

Fri Mar 21 00:00:00 UTC 2014

Doxycycline inhibits polarization of macrophages to the proangiogenic M2-type and subsequent neovascularization.

The Journal of biological chemistry

He L,Marneros AG

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Flow Cytometry

Fri Mar 21 00:00:00 UTC 2014

Glioma grade is associated with the accumulation and activity of cells bearing M2 monocyte markers.

Clinical cancer research : an official journal of the American Association for Cancer Research

Prosniak M,Harshyne LA,Andrews DW,Kenyon LC,Bedelbaeva K,Apanasovich TV,Heber-Katz E,Curtis MT,Cotzia P,Hooper DC

14-0689 was used in Flow cytometry/Cell sorting to show that the content of CD163(+) cells distinguishes grade III astrocytoma subsets with different prognosis.

Mon Jul 15 00:00:00 UTC 2013

Hepatocellular proliferation correlates with inflammatory cell and cytokine changes in a murine model of nonalchoholic fatty liver disease.

PloS one

Vansaun MN,Mendonsa AM,Lee Gorden D

Published figure using CD68 monoclonal antibody (Product # 14-0689-82) in Immunohistochemistry

Sun Jun 15 00:00:00 UTC 2014

A consensus surface activation marker signature is partially dependent on human immunodeficiency virus type 1 Nef expression within productively infected macrophages.

Retrovirology

Babu R,Brown A

14-0689 was used in Flow cytometry/Cell sorting to determine whether macrophage surface marker phenotype in non-polarised cultures is altered by HIV infection independently of host genetic background.

Mon Dec 16 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: CD68; CD68 antigen; Gp110; macrophage antigen CD68; Macrosialin; scavenger receptor class D, member 1

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Gene Aliases: CD68; GP110; LAMP4; SCARD1

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UniProt ID: (Human) P34810

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Entrez Gene ID: (Human) 968

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Process(es)

cellular response to organic substance

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), eBioscience™

CD68 Antibody (14-0689-82) in ICC/IF

Product Details

14-0689-82

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunomicroscopy (IM)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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