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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD69 Antibodies

          Invitrogen

          CD69 Monoclonal Antibody (H1.2F3), FITC, eBioscience™

          72 Published Figures
          108 References
          View all (85) CD69 antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD69 Monoclonal Antibody (H1.2F3), FITC, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (72)
          CD69 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD69 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 73

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          CD69 Antibody (11-0691-82) in Flow

          Staining of unstimulated (left) or overnight ConA-stimulated (right) C57BL/6 mouse splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control FITC (Product # 11-4888-81) (blue histogram) or 0.25 µg of Anti-Mouse CD69 FITC (purple histogram). Total viable cells were analyzed. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry, ELISA (Flow, ELISA)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)
          CD69 Antibody in Flow Cytometry (Flow)

          Product Details

          11-0691-82

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 106 publications 106 publications

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          In vitro Assay (IV)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Armenian hamster / IgG

          Recommended Isotype Control

          Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          H1.2F3

          Conjugate

          FITC FITC FITC
          • Unconjugated
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 496
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant Violet 421
          • Brilliant Violet 786
          • eFluor 450
          • Functional Grade
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Request custom conjugation

          Excitation/Emission Max

          498/517 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_465119

          Product Specific Information

          Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.

          Applications Reported: The H1.2F3 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: The H1.2F3 antibody has been tested by flow cytometric analysis of resting and activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD69; Early activation antigen CD69; Very Early Activation Antigen

          View more View less

          Gene Aliases: 5830438K24Rik; AI452015; AIM; Cd69; VEA

          View more View less

          UniProt ID: (Mouse) P37217

          View more View less

          Entrez Gene ID: (Mouse) 12515

          View more View less

          Function(s)
          calcium ion binding carbohydrate binding
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          We're here to help

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