Invitrogen

CD69 Monoclonal Antibody (H1.2F3)

CD69 Antibody in Immunocytochemistry (ICC/IF)

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CD69 Antibody (MA1-207) in ICC/IF

Immunofluorescent analysis of CD69 (green) in PHA-M (2% v/v, Product # 10576-015) treated Raji cells. Cells were blocked with blocking buffer (Product # 37525) for 30 min at room temparture and then stained with CD69 hamster monoclonal antibody (Product # MA1-207) at a dilution of 1:100 for 1 hour on ice. After washing with ice-cold PBS for 3 times, the cells were then stained with DyLight™ 488 Goat anti-hamster (Armenian) IgG antibody for 1 hour on ice. The cells were fixed with 4% paraformaldehyde for 30 minutes at room temperature. Nucl... View More

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CD69 Antibody — The specificity of anti-CD69 monoclonal antibody (Product # MA1-207) was demonstrated by the immunofluorescence detection of increased expression of CD69 in Raji cells upon treatment with PHA-M. {TM}

Product Details

MA1-207

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunocytochemistry (ICC/IF)

1:50-1:500

Flow Cytometry (Flow)

1:50-1:500

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

H1.2F3

Immunogen

Mouse dendritic epidermal T cell line Y245

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2637376

Product Specific Information

MA1-207 detects CD69 from human samples.

MA1-207 has been successfully tested in western blot, immunofluorescence and flow cytometry applications.

In western blot, MA1-207 detects CXCR1/IL8RA (human) at approximate molecular weight of 22.5 kDa.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression of a Chimeric Antigen Receptor Specific for Donor HLA Class I Enhances the Potency of Human Regulatory T Cells in Preventing Human Skin Transplant Rejection.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Boardman DA,Philippeos C,Fruhwirth GO,Ibrahim MA,Hannen RF,Cooper D,Marelli-Berg FM,Watt FM,Lechler RI,Maher J,Smyth LA,Lombardi G

MA1207 was used in flow cytometry to observe that the use of chimeric antigen receptor technology is a clinically applicable refinement of regulatory T cell therapy for organ transplantation

Sat Apr 01 00:00:00 UTC 2017

NK cells regulate CXCR2+ neutrophil recruitment during acute lung injury.

Journal of leukocyte biology

Hoegl S,Ehrentraut H,Brodsky KS,Victorino F,Golden-Mason L,Eltzschig HK,McNamee EN

MA1207 was used in flow cytometry to elucidate the role of Tbet in acute lung injury

Wed Feb 01 00:00:00 UTC 2017

C1q acts in the tumour microenvironment as a cancer-promoting factor independently of complement activation.

Nature communications

Bulla R,Tripodo C,Rami D,Ling GS,Agostinis C,Guarnotta C,Zorzet S,Durigutto P,Botto M,Tedesco F

MA1207 was used in flow cytometry to suggest that locally synthesized C1q promotes tumor growth

Mon Feb 01 00:00:00 UTC 2016

Aberrant germinal center formation, follicular T-helper cells, and germinal center B-cells were involved in chronic graft-versus-host disease.

Annals of hematology

Shao L,Lie AK,Zhang Y,Wong CH,Kwong YL

MA1207 was used in flow cytometry to explore the roles of T and B cells in chronic graft-versus-host disease

Tue Sep 01 00:00:00 UTC 2015

Lysophosphatidic acid receptor 5 inhibits B cell antigen receptor signaling and antibody response.

Journal of immunology (Baltimore, Md. : 1950)

Hu J,Oda SK,Shotts K,Donovan EE,Strauch P,Pujanauski LM,Victorino F,Al-Shami A,Fujiwara Y,Tigyi G,Oravecz T,Pelanda R,Torres RM

MA1-207 was used in Flow Cytometry to reveal that alterations in lysophosphatidic acid levels likely influence adaptive humoural immunity.

Tue Jul 01 00:00:00 UTC 2014

Effector CD4 T-cell transition to memory requires late cognate interactions that induce autocrine IL-2.

Nature communications

McKinstry KK,Strutt TM,Bautista B,Zhang W,Kuang Y,Cooper AM,Swain SL

MA1207 was used in flow cytometry to explore the interaction between IL-2 and IL-7 in CD4 T-cell memory generation

Wed Nov 05 00:00:00 UTC 2014

Arhgef1 is required by T cells for the development of airway hyperreactivity and inflammation.

American journal of respiratory and critical care medicine

Brown JP,Taube C,Miyahara N,Koya T,Pelanda R,Gelfand EW,Torres RM

MA1-207 was used in Flow Cytometry to evaluate a possible contribution for Arhgef1 in the development of airway inflammation and airway hyperreactivity.

Sun Jul 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: Activation inducer molecule; activation inducer molecule (AIM/CD69); AIM; BL-AC/P26; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; CD69; CD69 antigen (p60, early T-cell activation antigen); EA1; Early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; Very Early Activation Antigen

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Gene Aliases: 5830438K24Rik; AI452015; AIM; BL-AC/P26; CD69; CLEC2C; EA1; GP32/28; MLR-3; VEA

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UniProt ID: (Human) Q07108, (Mouse) P37217

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Entrez Gene ID: (Human) 969, (Mouse) 12515

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Function(s)

transmembrane signaling receptor activity calcium ion binding protein binding carbohydrate binding

Process(es)

signal transduction cellular response to drug

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD69 Monoclonal Antibody (H1.2F3)

CD69 Antibody (MA1-207) in ICC/IF

Product Details

MA1-207

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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