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Invitrogen
CD71 (Transferrin Receptor) Monoclonal Antibody (OX26), APC, eBioscience™
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FIGURE: 1 / 1
CD71 (Transferrin Receptor) Antibody (17-0710-82) in Flow
Staining of rat bone marrow cells with Anti-Rat CD45R (B220) PE (Product # 12-0460-82) and 0.125 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or 0.125 µg of Anti-Rat CD71 (Transferrin Receptor) APC (right). Total viable cells were used for analysis.
Product Details
17-0710-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OX26
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573153
Product Specific Information
Description: The monoclonal antibody OX26 reacts with rat CD71, which is also known as the transferrin receptor. This glycoprotein exists as a homodimer on the cell surface that facilitates the endocytosis of iron by binding to iron-saturated transferrin. CD71 is expressed by proliferating cells of hematopoietic lineages, but not by mature, resting cells or hematopoietic stem cells. Its increased expression, which can be induced by antigen and mitogen stimulation, has been shown to correlate with cell proliferation. Endothelial cells of brain capillaries, cortical neurons, oligodendrocytes, kidney tubules, hepatocytes, pancreatic cells, and muscle cells also express CD71.
The OX26 antibody has been reported to exhibit agonist activity.
Applications Reported: This OX26 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This OX26 antibody has been tested by flow cytometric analysis of rat bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: CD antigen CD71; CD71; tfR; tfR1; TR; Transferrin receptor protein 1
Gene Aliases: Tfrc; Trfr
UniProt ID: (Rat) Q99376
Entrez Gene ID: (Rat) 64678
regulation of cell growth
response to hypoxia
cellular iron ion homeostasis
receptor-mediated endocytosis
acute-phase response
aging
response to nutrient
response to organic substance
response to inorganic substance
response to iron ion
response to manganese ion
osteoclast differentiation
response to retinoic acid
transferrin transport
cellular response to drug
regulation of cell proliferation
positive regulation of bone resorption
response to copper ion
iron ion import
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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