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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Transferrin Receptor (CD71) Antibodies

          Invitrogen

          CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), PE, eBioscience™

          26 Published Figures
          50 References
          View all (135) Transferrin Receptor (CD71) antibodies

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          Cite CD71 (Transferrin Receptor) Monoclonal Antibody (R17217 (RI7 217.1.4)), PE, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (26)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 27

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          CD71 (Transferrin Receptor) Antibody (12-0711-82) in Flow

          Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.25 µg of Anti-Mouse CD71 (Transferrin Receptor) PE (right). Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)
          CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

          Product Details

          12-0711-82

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 50 publications 50 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Fish, Human, Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          R17217 (RI7 217.1.4)

          Conjugate

          PE PE PE
          • Unconjugated
          • Alexa Fluor 700 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • eFluor 450
          • FITC
          • NovaFluor Blue 610-70S
          • NovaFluor Blue 660-120S
          • NovaFluor Blue 690
          • NovaFluor Red 685
          • NovaFluor Red 710
          • NovaFluor Red 755
          • NovaFluor Yellow 690
          • NovaFluor Yellow 730
          • NovaFluor Yellow 755
          • PE-Cyanine7
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Request custom conjugation

          Excitation/Emission Max

          565/576 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_465740

          Product Specific Information

          Description: The R17217 monoclonal antibody reacts with mouse CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71. Expression level differences have been observed in different mouse strains.

          Applications Reported: The R17217 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: The R17217 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD antigen CD71; CD71; mammary tumor virus receptor 1; TR; Transferrin receptor protein 1

          View more View less

          Gene Aliases: 2610028K12Rik; AI195355; AI426448; AU015758; CD71; E430033M20Rik; Mtvr-1; Mtvr1; p90; TFR; TFR1; Tfrc; TR; Trfr

          View more View less

          UniProt ID: (Mouse) Q62351

          View more View less

          Entrez Gene ID: (Mouse) 22042

          View more View less

          Function(s)
          glycoprotein binding double-stranded RNA binding transferrin receptor activity iron ion transmembrane transporter activity protein binding Hsp70 protein binding transferrin transmembrane transporter activity identical protein binding poly(A) RNA binding chaperone binding
          Process(es)
          cellular iron ion homeostasis endocytosis receptor-mediated endocytosis osteoclast differentiation transferrin transport iron ion transmembrane transport cellular response to drug positive regulation of bone resorption protein transmembrane transport iron ion import
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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