Invitrogen

CD74 Polyclonal Antibody

CD74 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 12

CD74 Antibody (PA5-22113) in IHC (P)

Immunohistochemistry analysis of CD74 in paraffin-embedded human lung cancer. Samples were incubated with CD74 polyclonal antibody (Product # PA5-22113) using a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

CD74 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD74 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of CD74 using Anti-CD74 Polyclonal Antibody, (Product # PA5-22113) was observed in Daudi, REH and Raji when compared to K-562, HL-60, HEK-293 and BJ. {RE}

Product Details

PA5-22113

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CD74. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.61 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11157006

Product Specific Information

Recommended positive controls: Raji, GL261, Rat2.

Predicted reactivity: Chimpanzee (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Single-cell RNA-sequencing identifies disease-associated oligodendrocytes in male APP NL-G-F and 5XFAD mice.

Nature communications

Park H,Cho B,Kim H,Saito T,Saido TC,Won KJ,Kim J

PA5-22113 was used in Immunohistochemistry-immunofluorescence to study the pathological roles of oligodendrocytes in Alzheimer's disease (AD).

Mon Feb 13 00:00:00 UTC 2023

Single-cell RNA-sequencing identifies disease-associated oligodendrocytes in male APP NL-G-F and 5XFAD mice.

Nature communications

Park H,Cho B,Kim H,Saito T,Saido TC,Won KJ,Kim J

PA5-22113 was used in Immunohistochemistry-immunofluorescence to study the pathological roles of oligodendrocytes in Alzheimer's disease (AD).

Mon Feb 13 00:00:00 UTC 2023

Single-cell RNA-sequencing identifies disease-associated oligodendrocytes in male APP NL-G-F and 5XFAD mice.

Nature communications

Park H,Cho B,Kim H,Saito T,Saido TC,Won KJ,Kim J

PA5-22113 was used in Immunohistochemistry-immunofluorescence to study the pathological roles of oligodendrocytes in Alzheimer's disease (AD).

Mon Feb 13 00:00:00 UTC 2023

Commensal microbiota divergently affect myeloid subsets in the mammalian central nervous system during homeostasis and disease.

The EMBO journal

Sankowski R,Ahmari J,Mezö C,Hrabě de Angelis AL,Fuchs V,Utermöhlen O,Buch T,Blank T,Gomez de Agüero M,Macpherson AJ,Erny D

PA5-22113 was used in Immunohistochemistry to assess central nervous system-associated macrophages in a more chronic pathological state in 5xFAD mice, a model for Alzheimer's disease, and found enhanced amyloid beta uptake exclusively by perivascular macrophages in germ-free 5xFAD mice.

Wed Dec 01 00:00:00 UTC 2021

Commensal microbiota divergently affect myeloid subsets in the mammalian central nervous system during homeostasis and disease.

The EMBO journal

Sankowski R,Ahmari J,Mezö C,Hrabě de Angelis AL,Fuchs V,Utermöhlen O,Buch T,Blank T,Gomez de Agüero M,Macpherson AJ,Erny D

Wed Dec 01 00:00:00 UTC 2021

MIF-2/D-DT enhances proximal tubular cell regeneration through SLPI- and ATF4-dependent mechanisms.

American journal of physiology. Renal physiology

Ochi A,Chen D,Schulte W,Leng L,Moeckel N,Piecychna M,Averdunk L,Stoppe C,Bucala R,Moeckel G

PA5-22113 was used in Immunohistochemistry to examine the role of MIF-2/D-DT, which mediates tissue protection in the heart, in tubular cell regeneration from ischemiareperfusion injury.

Fri Sep 01 00:00:00 UTC 2017

MIF-2/D-DT enhances proximal tubular cell regeneration through SLPI- and ATF4-dependent mechanisms.

American journal of physiology. Renal physiology

Ochi A,Chen D,Schulte W,Leng L,Moeckel N,Piecychna M,Averdunk L,Stoppe C,Bucala R,Moeckel G

PA5-22113 was used in Immunohistochemistry to examine the role of MIF-2/D-DT, which mediates tissue protection in the heart, in tubular cell regeneration from ischemiareperfusion injury.

Fri Sep 01 00:00:00 UTC 2017

Immune-modulating Activity of Hydrogel Microparticles Contributes to the Host Defense in a Murine Model of Cutaneous Anthrax.

Frontiers in molecular biosciences

Teunis AL,Popova TG,Espina V,Liotta LA,Popov SG

Published figure using CD74 polyclonal antibody (Product # PA5-22113) in Immunohistochemistry (Paraffin)

Mon Sep 28 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: CD74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; class II-associated invariant chain peptide; dinucleotide microsatellite; gamma chain of class II antigens; H-2 class II histocompatibility antigen gamma chain; histocompatibility: class II antigens, gamma chain of; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; HLA-DR-GAMMA; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ii; invariant polypeptide of major histocompatibility complex, class II antigen-associated; MHC class II-associated invariant chain; MHC HLA-DR gamma chain; p33

View more View less

Gene Aliases: CD74; CLIP; DHLAG; HLADG; Ia-GAMMA; II; INVG34

View more View less

UniProt ID: (Human) P04233, (Mouse) P04441, (Rat) P10247

View more View less

Entrez Gene ID: (Human) 972, (Mouse) 16149, (Rat) 25599

View more View less

Function(s)

beta-amyloid binding cytokine receptor activity protein binding cytokine binding MHC class II protein complex binding macrophage migration inhibitory factor binding MHC class II protein binding MHC class II protein binding, via antigen binding groove identical protein binding protein binding involved in protein folding nitric-oxide synthase binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD74 Polyclonal Antibody

CD74 Antibody (PA5-22113) in IHC (P)

Product Details

PA5-22113

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!