Invitrogen

CD74 Chimeric Recombinant Rabbit Monoclonal Antibody (LN-2)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-48279

Applications
Tested Dilution
Publications

Western Blot (WB)

0.0001 µg/mL
-

Immunohistochemistry (IHC)

Assay-dependent
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-

Flow Cytometry (Flow)

1:100
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

LN-2

Immunogen

This antibody was raised by immunizing mice with SU-DHL-4 lymphoma cells.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3074721

Product Specific Information

Specificity: LN-2 antibody recognizes CD74. It reacts with an epitope residing within 60 amino acids of the extracytoplasmic, COOH terminus of the protein. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides.

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; class II-associated invariant chain peptide; dinucleotide microsatellite; gamma chain of class II antigens; H-2 class II histocompatibility antigen gamma chain; histocompatibility: class II antigens, gamma chain of; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; HLA-DR-GAMMA; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ii; invariant polypeptide of major histocompatibility complex, class II antigen-associated; MHC class II-associated invariant chain; MHC HLA-DR gamma chain; p33

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Gene Aliases: CD74; CLIP; DHLAG; HLADG; Ia-GAMMA; II; INVG34

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UniProt ID: (Human) P04233, (Mouse) P04441, (Rat) P10247

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Entrez Gene ID: (Human) 972, (Mouse) 16149, (Rat) 25599

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Function(s)
beta-amyloid binding cytokine receptor activity protein binding cytokine binding MHC class II protein complex binding macrophage migration inhibitory factor binding MHC class II protein binding MHC class II protein binding, via antigen binding groove identical protein binding protein binding involved in protein folding nitric-oxide synthase binding
Process(es)
activation of MAPK activity prostaglandin biosynthetic process positive regulation of cytokine-mediated signaling pathway immune system process positive regulation of dendritic cell antigen processing and presentation negative regulation of peptide secretion positive regulation of adaptive immune response positive regulation of type 2 immune response negative regulation of mature B cell apoptotic process protein folding protein complex assembly intracellular protein transport defense response immune response signal transduction cell proliferation immunoglobulin mediated immune response cytokine-mediated signaling pathway antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class II positive regulation of B cell proliferation macrophage migration inhibitory factor signaling pathway negative regulation of apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator positive thymic T cell selection negative thymic T cell selection positive regulation of innate immune response negative regulation of T cell differentiation positive regulation of T cell differentiation positive regulation of fibroblast proliferation positive regulation of peptidyl-tyrosine phosphorylation chaperone mediated protein folding requiring cofactor positive regulation of macrophage cytokine production positive regulation of ERK1 and ERK2 cascade positive regulation of neutrophil chemotaxis negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of chemokine (C-X-C motif) ligand 2 production antigen processing and presentation of endogenous antigen regulation of macrophage activation T cell selection leukocyte migration
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