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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD8 Antibodies

          Invitrogen

          CD8 Monoclonal Antibody (3B5), Pacific Orange™

          3 Published Figures
          33 References
          View all (529) CD8 antibodies

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          Datasheet
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          Datasheet
          Protocols
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          Cite CD8 Monoclonal Antibody (3B5), Pacific Orange™

          • Antibody Testing Data (2)
          • Published Figures (3)
          CD8 Antibody in Flow Cytometry (Flow)
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          CD8 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          CD8 Antibody (MHCD0830) in Flow

          Human peripheral blood lymphocytes were stained using Pacific Orange-8 fluorochrome conjugates of anti-uman CD8 monoclonal antibody (clone 3B5). The negative control profiles represent unstained cells. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          CD8 Antibody in Flow Cytometry (Flow)
          CD8 Antibody in Flow Cytometry (Flow)
          CD8 Antibody in Flow Cytometry (Flow)
          CD8 Antibody in Flow Cytometry (Flow)
          CD8 Antibody in Flow Cytometry (Flow)

          Product Details

          MHCD0830

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          Assay-Dependent
          View 19 publications 19 publications

          Miscellaneous PubMed (MISC)

          -
          View 14 publications 14 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Non-human primate, Rhesus monkey

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          3B5

          Immunogen

          Human CD8

          Conjugate

          Pacific Orange™ Pacific Orange™ Pacific Orange™
          • Unconjugated
          • Alexa Fluor 405 
          • Alexa Fluor 647 
          • Alexa Fluor 700 
          • APC 
          • APC-Alexa Fluor 750
          • FITC
          • Pacific Blue
          • PE 
          • PE-Alexa Fluor 610
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-Texas Red
          • PerCP 
          • Qdot 605
          • Qdot 655
          • Qdot 705
          • Qdot 800
          • TRI-COLOR
          • Request custom conjugation

          Excitation/Emission Max

          400/551 nm View spectra spectra

          Form

          Liquid

          Purification

          purified

          Storage buffer

          PBS with BSA

          Contains

          0.1% sodium azide

          Storage conditions

          4°C, store in dark

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10372066

          Product Specific Information

          Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.

          This antibody recognizes the alpha chain alone as well as the alpha/beta heterodimer.

          Target Information

          Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

          Analyte Specific Reagent

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

          View more View less

          Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

          View more View less

          UniProt ID: (Human) P01732, (Human) P10966

          View more View less

          Entrez Gene ID: (Human) 925, (Human) 926

          View more View less

          Function(s)
          protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity
          Process(es)
          adaptive immune response immune response transmembrane receptor protein tyrosine kinase signaling pathway T cell activation regulation of defense response to virus by virus regulation of immune response T cell mediated immunity cell surface receptor signaling pathway antigen processing and presentation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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