Invitrogen

CD8 Monoclonal Antibody (3B5), Qdot™ 655

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CD8 Antibody (Q10055) in Flow

Qdot® 655 anti-human CD8 conjugate 655/20 band pass filter

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Product Details

Q10055

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

Recognizes the CD8 antigen

Conjugate

Qdot™ 655 Qdot™ 655 Qdot™ 655

Excitation/Emission Max

300/654 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

0.05M borate, pH 8.3, with 1M betaine

Contains

0.05% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2556445

Product Specific Information

Qdot™ Antibody (Ab) conjugates possess a bright fluorescence emission that makes them well suited for the detection of low-abundance extracellular proteins. Approximately the same size as R-phycoerythrin (R-PE) and compatible with existing organic fluorophore conjugates, Qdot™ Ab conjugates can be excited with any wavelength below their emission maximum, but are best excited by UV or violet light. The narrow, symmetric emission profiles of Qdot Ab conjugates allow for minimal compensation when using a single excitation source, and the very long stoke shifts enable better, more efficient multicolor assays using the 405 nm violet laser. Available in multiple colors for use in flow cytometry, these advantages make Qdot Ab conjugates powerful tools for antibody labeling and staining. Staining: Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA). We recommend analysis of cells within 18 hours of staining. If dilute reagent is used, dilute only the quantity of reagent to be used within one day. Qdot Ab conjugates may be mixed with other antibodies, but use the diluted conjugates on the day of dilution. Qdot Ab conjugates can be used for surface staining applications with most conventional sample preparation reagents, such as Cal-Lyse™ Lysing Solution and FIX & PERM™ reagents, with minimal effect on fluorescence. We have observed some batches of BD FACS™ Lysing Solution to interfere with Qdot Ab conjugate fluorescence. Each lot has been tested by flow cytometry using human peripheral blood leukocytes. This antibody recognizes the CD8 antigen, also known as T8 and Lyt2, and recognizes the alpha chain alone as well as the alpha-beta heterodimer. The isotype control for this antibody is mouse IgG2a, Cat. No. Q10015.

Instrument setup: Qdot Ab conjugates are excited optimally with UV or 405 nm light, although excitation can be obtained with any wavelength below the emission maximum of a given Qdot™ nanocrystal, such as with a 488-nm laser. Qdot Ab conjugates can be used on cytometers that do not have UV or violet excitation sources as long as they have appropriate emission filters. Make sure the cytometer has an appropriate emission filter for the Qdot Ab conjugate being used; alternate filters can be used as long as they capture the emission maximum, but filter width impacts spectral overlap corrections. And be sure to check for Qdot Ab conjugate emission in any channel that can capture nanocrystal emission off of other lasers on the cytometer. For Cat. No. Q10055: peak excitation 405 (488) nm/peak emission 655 nm; recommended filter 655/20 nm.

Store reagents at 2-8°C in the dark. Do not freeze. Because Qdot nanocrystals are conjugated to biological materials, some loss of activity may be observed with prolonged storage. When stored as instructed, expires six months from date of receipt unless otherwise indicated on product label. Qdot Ab conjugates are photostable, and do not need to be protected from light. However, if using Qdot Ab conjugates in combination with conventional fluorochrome conjugated antibodies, minimize light exposure during handling, incubation with cells, and prior to analysis. The Qdot Ab conjugates contain cadmium and selenium in an inorganic crystalline form. Dispose of the material in compliance with all applicable local, state, and federal regulations for disposal of these classes of material. For more information on the composition of these materials, consult the Safety Data Sheets (SDSs).

Target Information

CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

HIV silencing and cell survival signatures in infected T cell reservoirs.

Nature

Clark IC,Mudvari P,Thaploo S,Smith S,Abu-Laban M,Hamouda M,Theberge M,Shah S,Ko SH,Pérez L,Bunis DG,Lee JS,Kilam D,Zakaria S,Choi S,Darko S,Henry AR,Wheeler MA,Hoh R,Butrus S,Deeks SG,Quintana FJ,Douek DC,Abate AR,Boritz EA

Q10055 was used in Flow Cytometry to reveal that HIV-infected memory CD4 T cells under antiretroviral therapy are a distinctive population with host gene expression patterns that favour HIV silencing, cell survival and cell proliferation, with important implications for the development of HIV cure strategies.

Wed Feb 01 00:00:00 UTC 2023

HIV silencing and cell survival signatures in infected T cell reservoirs.

Nature

Wed Feb 01 00:00:00 UTC 2023

HIV Infected T Cells Can Proliferate in vivo Without Inducing Expression of the Integrated Provirus.

Frontiers in microbiology

Musick A,Spindler J,Boritz E,Pérez L,Crespo-Vélez D,Patro SC,Sobolewski MD,Bale MJ,Reid C,Keele BF,Capoferri A,Shao W,Wiegand A,Simonetti FR,Mellors JW,Hughes SH,Coffin JM,Maldarelli F,Kearney MF

Q10055 was used in Flow cytometry/Cell sorting to show that the long, presumed correlation between the level of cellular and proviral activation may not be accurate and, therefore, requires further investigation.

Sat Jun 15 00:00:00 UTC 2024

Effect of chronic morphine administration on circulating dendritic cells in SIV-infected rhesus macaques.

Journal of neuroimmunology

Cornwell WD,Wagner W,Lewis MG,Fan X,Rappaport J,Rogers TJ

Q10055 was used in Flow cytometry/Cell sorting to study the effect of chronic morphine administration on the circulating dendritic cell population dynamics associated with SIV infection.

Wed Jun 15 00:00:00 UTC 2016

Anti-α4 antibody treatment blocks virus traffic to the brain and gut early, and stabilizes CNS injury late in infection.

PLoS pathogens

Campbell JH,Ratai EM,Autissier P,Nolan DJ,Tse S,Miller AD,González RG,Salemi M,Burdo TH,Williams KC

Q10055 was used in Flow cytometry/Cell sorting to show that leukocyte traffic early in infection seeds the CNS with virus and contributes to productive infection in the gut in SIV-infected monkeys.

Mon Dec 01 00:00:00 UTC 2014

Overweight and obese adult humans have a defective cellular immune response to pandemic H1N1 influenza A virus.

Obesity (Silver Spring, Md.)

Paich HA,Sheridan PA,Handy J,Karlsson EA,Schultz-Cherry S,Hudgens MG,Noah TL,Weir SS,Beck MA

Q10055 was used in flow cytometry to study defective cellular immune response to pandemic H1N1 influenza A virus in obese and overweight adult humans

Fri Nov 01 00:00:00 UTC 2013

Impact of antibody quality and anamnestic response on viremia control post-challenge in a combined Tat/Env vaccine regimen in rhesus macaques.

Virology

Demberg T,Brocca-Cofano E,Kuate S,Aladi S,Vargas-Inchaustegui DA,Venzon D,Kalisz I,Kalyanaraman VS,Lee EM,Pal R,DiPasquale J,Ruprecht RM,Montefiori DC,Srivastava I,Barnett SW,Robert-Guroff M

Q10055 was used in flow cytometry to use Tat, Env, and Tat/Env regimens to induce and immune response and then assess protective efficacy using clade C Env, alum adjuvant, and a heterologous intrarectal SHIV1157ipd3N4 challenge

Wed Jun 05 00:00:00 UTC 2013

Decreased interleukin 7 responsiveness of T lymphocytes in patients with idiopathic CD4 lymphopenia.

The Journal of infectious diseases

Puronen CE,Thompson WL,Imamichi H,Beq S,Hodge JN,Rehm C,Parker R,DerSimonian R,Brenchley JM,Sereti I

Q10055 was used in flow cytometry to discuss the link between interleukin 7 and lymphopenia

Tue May 01 00:00:00 UTC 2012

Classical ataxia telangiectasia patients have a congenitally aged immune system with high expression of CD95.

Journal of immunology (Baltimore, Md. : 1950)

Carney EF,Srinivasan V,Moss PA,Taylor AM

Q10055 was used in flow cytometry to assess the effect of having ataxia-telangiectasia and age on CD95 and IL-7 expression

Sun Jul 01 00:00:00 UTC 2012

Cross-reactivity and expansion of dengue-specific T cells during acute primary and secondary infections in humans.

Scientific reports

Friberg H,Bashyam H,Toyosaki-Maeda T,Potts JA,Greenough T,Kalayanarooj S,Gibbons RV,Nisalak A,Srikiatkhachorn A,Green S,Stephens HA,Rothman AL,Mathew A

Q10055 was used in flow cytometry to examine epitope-specific T cell responses in subjects with primary or secondary dengue virus infection

Wed Aug 28 00:00:00 UTC 2013

Evaluation of a 12-color flow cytometry panel to study lymphocyte, monocyte, and dendritic cell subsets in humans.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Autissier P,Soulas C,Burdo TH,Williams KC

Q10055 was used in flow cytometry to use flow cytometry to characterize human lymphocyte, monocyte, and DC subsets

Sat May 01 00:00:00 UTC 2010

Immunophenotyping of lymphocyte, monocyte and dendritic cell subsets in normal rhesus macaques by 12-color flow cytometry: clarification on DC heterogeneity.

Journal of immunological methods

Autissier P,Soulas C,Burdo TH,Williams KC

Q10055 was used in flow cytometry to use flow cytometry to characterize rhesus macaque lymphocyte, monocyte and dendritic cell subsets

Tue Aug 31 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity

Process(es)

adaptive immune response immune response transmembrane receptor protein tyrosine kinase signaling pathway T cell activation regulation of defense response to virus by virus regulation of immune response T cell mediated immunity cell surface receptor signaling pathway antigen processing and presentation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8 Monoclonal Antibody (3B5), Qdot™ 655

CD8 Antibody (Q10055) in Flow

Product Details

Q10055

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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