Invitrogen

CD8 alpha Monoclonal Antibody (53-6.7), FITC

5 Published Figures

8 References

View all (356) CD8 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Published Figures (5)

CD8 alpha Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 7

CD8 alpha Antibody (MA1-10303) in Flow

Separation of murine CD8a positive splenocytes (red-filled) from CD8a negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) FITC Monoclonal antibody (Product # MA1-10303) (concentration in sample 1 µg/mL).

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Product Details

MA1-10303

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1-2 µg/mL

View 5 publications 5 publications

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

53-6.7

Immunogen

Mouse spleen cells

View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11153636

Product Specific Information

This antibody recognizes an extracellular epitope of murine CD8a (32-34 kDa; alpha chain of the CD8 antigen).

Target Information

CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Valosin-containing protein (VCP/p97) inhibition reduces viral clearance and induces toxicity associated with muscular damage.

Cell death & disease

Del Rio Oliva M,Basler M

MA1-10303 was used in Flow Cytometry to evaluate the effect of CB-5083 on the immune response in mice by using the lymphocytic choriomeningitis virus (LCMV) as an infection model.

Thu Dec 01 00:00:00 UTC 2022

Valosin-containing protein (VCP/p97) inhibition reduces viral clearance and induces toxicity associated with muscular damage.

Cell death & disease

Del Rio Oliva M,Basler M

MA1-10303 was used in Flow Cytometry to evaluate the effect of CB-5083 on the immune response in mice by using the lymphocytic choriomeningitis virus (LCMV) as an infection model.

Thu Dec 01 00:00:00 UTC 2022

Repigmentation by combined narrow‑band ultraviolet B/adipose‑derived stem cell transplantation in the mouse model: Role of Nrf2/HO‑1‑mediated Ca²⁺ homeostasis.

Molecular medicine reports

Bian Y,Yu H,Jin M,Gao X

MA1-10303 was used in Flow Cytometry to explore the underlying mechanisms and improve the understanding of the pathogenesis of vitiligo.

Sat Jan 01 00:00:00 UTC 2022

T-Bet Expression Mediated by the mTOR Pathway Influences CD4⁺ T Cell Count in Mice With Lethal Candida Sepsis.

Frontiers in microbiology

Bai G,Wang H,Han W,Cui N

MA1-10303 was used in Flow cytometry/Cell sorting to establish the influence of T-bet expression on CD4+ T cell count in Candida sepsis.

Fri Nov 03 00:00:00 UTC 2023

T-Bet Expression Mediated by the mTOR Pathway Influences CD4⁺ T Cell Count in Mice With Lethal Candida Sepsis.

Frontiers in microbiology

Bai G,Wang H,Han W,Cui N

MA1-10303 was used in Flow cytometry/Cell sorting to establish the influence of T-bet expression on CD4+ T cell count in Candida sepsis.

Fri Nov 03 00:00:00 UTC 2023

Enhanced protection to Mycobacterium tuberculosis infection in IL-10-deficient mice is accompanied by early and enhanced Th1 responses in the lung.

European journal of immunology

Redford PS,Boonstra A,Read S,Pitt J,Graham C,Stavropoulos E,Bancroft GJ,O'Garra A

MA1-10303 was used in Flow cytometry/Cell sorting to demonstrate that Mycobacterium tuberculosis infection in the absence of IL-10 results in reduced bacterial loads in the lung.

Sun Aug 01 00:00:00 UTC 2010

Role of T cells in innate and adaptive immunity against murine Burkholderia pseudomallei infection.

The Journal of infectious diseases

Haque A,Easton A,Smith D,O'Garra A,Van Rooijen N,Lertmemongkolchai G,Titball RW,Bancroft GJ

MA1-10303 was used in flow cytometry to study the in vivo role of cytokine- and antigen-mediated T cell activation in the resistance to Burkholderia pseudomallei

Wed Feb 01 00:00:00 UTC 2006

The source of early IFN-gamma that plays a role in Th1 priming.

Journal of immunology (Baltimore, Md. : 1950)

Das G,Sheridan S,Janeway CA

MA1-10303 was used in flow cytometry to identify the source of the early IFN-gamma after injection with anti-CD3.

Wed Aug 15 00:00:00 UTC 2001

Oral administration of the bacterial superantigen staphylococcal enterotoxin B induces activation and cytokine production by T cells in murine gut-associated lymphoid tissue.

Journal of immunology (Baltimore, Md. : 1950)

Spiekermann GM,Nagler-Anderson C

MA1-10303 was used in flow cytometry to compare gut and peripheral T cell responses after staphylococcal enterotoxin treatment.

Tue Dec 01 00:00:00 UTC 1998

B7 blockade prevents activation-induced cell death of thymocytes.

International immunology

Samoilova EB,Horton JL,Bassiri H,Zhang H,Linsley PS,Carding SR,Chen Y

MA1-10303 was used in flow cytometry to elucidate the role of B7 in thymic selection.

Sat Nov 01 00:00:00 UTC 1997

Bioinformatics

Protein Aliases: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

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Gene Aliases: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

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UniProt ID: (Mouse) P01731, (Mouse) P10300

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Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

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Function(s)

protein binding protein kinase binding protein homodimerization activity

Process(es)

immune system process T cell mediated immunity cell surface receptor signaling pathway T cell activation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus

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CD8 alpha Monoclonal Antibody (53-6.7), FITC

CD8 alpha Antibody (MA1-10303) in Flow

Product Details

MA1-10303

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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