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          • Primary Antibodies ›
          • CD8 Antibodies

          Invitrogen

          CD8 alpha Monoclonal Antibody (C8/144B)

          10 Published Figures
          51 References
          View all (529) CD8 antibodies

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          Cite CD8 alpha Monoclonal Antibody (C8/144B)

          • Antibody Testing Data (2)
          • Published Figures (10)
          CD8 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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          CD8 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 12

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          CD8 alpha Antibody (MA5-13473) in IHC (P)

          Formalin-fixed, paraffin-embedded human tonsil stained with CD8 antibody using peroxidase-conjugate and DAB chromogen. Note membrane staining of T cells. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD8 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD8 alpha Antibody in Flow Cytometry (Flow)
          CD8 alpha Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          CD8 alpha Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          CD8 alpha Antibody in Immunohistochemistry (IHC)
          CD8 alpha Antibody in Immunohistochemistry (IHC)
          CD8 alpha Antibody in Immunohistochemistry (IHC)
          CD8 alpha Antibody in Immunohistochemistry (IHC)
          CD8 alpha Antibody in Immunohistochemistry (IHC)
          CD8 alpha Antibody in Immunohistochemistry (IHC)
          CD8 alpha Antibody in Immunohistochemistry (IHC)
          CD8 alpha Antibody in Immunohistochemistry (IHC)

          Product Details

          MA5-13473

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 35 publications 35 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:25-1:50
          View 4 publications 4 publications

          Immunocytochemistry (ICC/IF)

          -
          View 6 publications 6 publications

          Flow Cytometry (Flow)

          1:2
          View 2 publications 2 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Luminex (LUM)

          -
          View 1 publication 1 publication

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          C8/144B

          Immunogen

          A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_11000353

          Product Specific Information

          MA5-13473 targets CD8 in IHC (P) and FACSs applications and shows reactivity with Human samples.

          The MA5-13473 immunogen is a 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of alpha chain of human CD8 molecule.

          Target Information

          Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

          View more View less

          Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

          View more View less

          UniProt ID: (Human) P01732, (Human) P10966

          View more View less

          Entrez Gene ID: (Human) 925, (Human) 926

          View more View less

          Function(s)
          protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity
          Process(es)
          adaptive immune response immune response transmembrane receptor protein tyrosine kinase signaling pathway T cell activation regulation of defense response to virus by virus regulation of immune response T cell mediated immunity cell surface receptor signaling pathway antigen processing and presentation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus
          It has to be done as per old AB suggested Products section.
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