Invitrogen

CD8 alpha Monoclonal Antibody (CT-CD8a), FITC

CD8 alpha Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD8 alpha Antibody (MA5-17597) in Flow

Flow cytometric analysis of C57BL/6 mouse splenic T-cells (left) or thymocytes (right) were stained using a CD8a/T subset NK monoclonal antibody (Product # MA5-17597) (filled histogram) or rat IgG2a isotype control (open histogram).

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Product Details

MA5-17597

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1.0 µg/1x10^6 cells

View 9 publications 9 publications

Miscellaneous PubMed (MISC)

View 14 publications 14 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

CT-CD8a

Immunogen

Mouse CD8a

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 4-5mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2538987

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Collateral Damage: What Effect Does Anti-CD4 and Anti-CD8α Antibody-Mediated Depletion Have on Leukocyte Populations?

Journal of immunology (Baltimore, Md. : 1950)

Jung SR,Suprunenko T,Ashhurst TM,King NJC,Hofer MJ

Mon Oct 01 00:00:00 UTC 2018

Inactivation of tumor-specific CD8⁺ CTLs by tumor-infiltrating tolerogenic dendritic cells.

Immunology and cell biology

Harimoto H,Shimizu M,Nakagawa Y,Nakatsuka K,Wakabayashi A,Sakamoto C,Takahashi H

MA5-17597 was used in flow cytometry to elucidate the role of dendritic cells in cancer immunosurveillance failure

Tue Oct 01 00:00:00 UTC 2013

Developmental dynamics of post-selection thymic DN iNKT.

PloS one

Yassai M,Cooley B,Gorski J

MA5-17597 was used in flow cytometry to study the kinetics of iNKT cell precursors

Tue Jan 29 00:00:00 UTC 2013

Gut ischemia-reperfusion affects gut mucosal immunity: a possible mechanism for infectious complications after severe surgical insults.

Critical care medicine

Fukatsu K,Sakamoto S,Hara E,Ueno C,Maeshima Y,Matsumoto I,Mochizuki H,Hiraide H

MA5-17597 was used in flow cytometry to determine the influence of gut ischemia/reperfusion on gut-associated lymphoid tissue mass and function

Sun Jan 01 00:00:00 UTC 2006

A protocol for mosaic analysis with a repressible cell marker (MARCM) in Drosophila.

Nature protocols

Wu JS,Luo L

MA5-17597 was used in immunocytochemistry to discuss the uses of mosaic analysis with a repressible cell marker (MARCM)

Mon Nov 05 00:00:00 UTC 2007

Dendritic cell expansion occurs in mesenteric lymph nodes of B10.BR mice infected with the murine nematode parasite Trichuris muris.

Parasitology research

Koyama K

MA5-17597 was used in flow cytometry to characterize dendritic cells in mice infected with Trichuris muris

Sat Oct 01 00:00:00 UTC 2005

Persistence of recipient lymphocytes in NOD mice after irradiation and bone marrow transplantation.

Journal of autoimmunity

Steptoe RJ,Stankovic S,Lopaticki S,Jones LK,Harrison LC,Morahan G

MA5-17597 was used in flow cytometry to analyze NOD mice after irradiation and bone marrow transplantation and the persistence of recipient lymphocytes

Mon Mar 01 00:00:00 UTC 2004

A transgenic mouse model for T-cell ignorance of a glial autoantigen.

Journal of autoimmunity

Cabarrocas J,Piaggio E,Zappulla JP,Desbois S,Mars LT,Lassmann H,Liblau RS

MA5-17597 was used in flow cytometry to determine the fate of autoreactive CD4+T cells in HNT-TCR x GFAP-HA double transgenic mice

Sat May 01 00:00:00 UTC 2004

Mycobacterial dissemination and cellular responses after 1-lobe restricted tuberculosis infection of genetically susceptible and resistant mice.

The Journal of infectious diseases

Mischenko VV,Kapina MA,Eruslanov EB,Kondratieva EV,Lyadova IV,Young DB,Apt AS

MA5-17597 was used in flow cytometry to study mycobacterial dissemination and immune-cell trafficking in tuberculosis

Wed Dec 15 00:00:00 UTC 2004

Lung cell responses to M. tuberculosis in genetically susceptible and resistant mice following intratracheal challenge.

Clinical and experimental immunology

Eruslanov EB,Majorov KB,Orlova MO,Mischenko VV,Kondratieva TK,Apt AS,Lyadova IV

MA5-17597 was used in flow cytometry to identify cellular mechanisms that contribute to susceptibility/resistance to tuberculosis.

Thu Jan 01 00:00:00 UTC 2004

Suppressor of cytokine signaling-1 regulates acute inflammatory arthritis and T cell activation.

The Journal of clinical investigation

Egan PJ,Lawlor KE,Alexander WS,Wicks IP

MA5-17597 was used in flow cytometry to examine the role of SOCS-1 in regulating inflammatory and immune responses in acute inflammatory arthritis.

Sat Mar 01 00:00:00 UTC 2003

Optimal colocalization of TCR and CD8 as a novel mechanism for the control of functional avidity.

Journal of immunology (Baltimore, Md. : 1950)

Cawthon AG,Alexander-Miller MA

MA5-17597 was used in flow cytometry to investigate the control of functional avidity in response to viral infection.

Tue Oct 01 00:00:00 UTC 2002

NK1.1+ cell depletion in vivo fails to prevent protection against infection with the murine nematode parasite Trichuris muris.

Parasite immunology

Koyama K

MA5-17597 was used in flow cytometry to test if NKT cells produce IL-4 in response to T. muris infection and induce an Th2 response.

Tue Jun 03 00:00:00 UTC 2003

Allogeneic bone marrow cells that facilitate complete chimerism and eliminate tumor cells express both CD8 and T-cell antigen receptor-alphabeta.

Blood

Lan F,Zeng D,Huie P,Higgins JP,Strober S

MA5-17597 was used in flow cytometry to test if specific subsets of cells in allogeneic bone marrow transplants can treat the BCL(1) B-cell lymphoma without inducing graft verses host disease.

Fri Jun 01 00:00:00 UTC 2001

Inhibition of thymidine synthesis by folate analogues induces a Fas-Fas ligand-independent deletion of superantigen-reactive peripheral T cells.

International immunology

Izeradjene K,Revillard JP,Genestier L

MA5-17597 was used in flow cytometry to assess the effects of folate analogues on superantigen-reactive peripheral T cells in vivo.

Mon Jan 01 00:00:00 UTC 2001

Increased thymic B cells but maintenance of thymic structure, T cell differentiation and negative selection in lymphotoxin-alpha and TNF gene-targeted mice.

Developmental immunology

Grech AP,Riminton DS,Gabor MJ,Hardy CL,Sedgwick JD,Godfrey DI

MA5-17597 was used in Flow Cytometry to show that, despite the presence of these cytokines and their receptors in the normal thymus, there appears no essential role for either TNF or LT in development of organ structure or for those processes associated with T cell repertoire selection.

Thu Jul 26 00:00:00 UTC 2001

Bone marrow NK1.1(-) and NK1.1(+) T cells reciprocally regulate acute graft versus host disease.

The Journal of experimental medicine

Zeng D,Lewis D,Dejbakhsh-Jones S,Lan F,García-Ojeda M,Sibley R,Strober S

MA5-17597 was used in flow cytometry to determine the contribution of NK1.1(-) and NK1.1(+) T cell subsets to GVHD.

Mon Apr 05 00:00:00 UTC 1999

Immunophenotypical and functional heterogeneity of dendritic cells generated from murine bone marrow cultured with different cytokine combinations: implications for anti-tumoral cell therapy.

Immunology

Masurier C,Pioche-Durieu C,Colombo BM,Lacave R,Lemoine FM,Klatzmann D,Guigon M

MA5-17597 was used in flow cytometry to characterize in vitro-generated dendritic cells.

Thu Apr 01 00:00:00 UTC 1999

Depletion of CD8+ cells abolishes memory in acquired immunity against Chlamydia pneumoniae in BALB/c mice.

Immunology

Penttilä JM,Anttila M,Varkila K,Puolakkainen M,Sarvas M,Mäkelä PH,Rautonen N

MA5-17597 was used in flow cytometry to determine the importance of T cells in Chlamydia pneumoniae infection.

Thu Jul 01 00:00:00 UTC 1999

CD8+ T cells become nonresponsive (anergic) following activation in the presence of costimulation.

Journal of immunology (Baltimore, Md. : 1950)

Deeths MJ,Kedl RM,Mescher MF

MA5-17597 was used in flow cytometry to study activation-induced nonresponsiveness of T cells.

Thu Jul 01 00:00:00 UTC 1999

Viral superantigen-induced negative selection of TCR transgenic CD4+ CD8+ thymocytes depends on activation, but not proliferation.

Blood

Ferrero I,Anjuère F,Azcoitia I,Renno T,MacDonald HR,Ardavín C

MA5-17597 was used in flow cytometry to analyze the cell activation and proliferation requirements for the deletion of viral superantigen-reactive Vbeta8.1 T-cell receptor transgenic thymocytes.

Mon Jun 01 00:00:00 UTC 1998

Genetic regulation of commitment to interleukin 4 production by a CD4(+) T cell-intrinsic mechanism.

The Journal of experimental medicine

Bix M,Wang ZE,Thiel B,Schork NJ,Locksley RM

MA5-17597 was used in flow cytometry to use T cells from BALB mice and study IL-4 production.

Mon Dec 21 00:00:00 UTC 1998

Three populations of mouse lymph node dendritic cells with different origins and dynamics.

Journal of immunology (Baltimore, Md. : 1950)

Salomon B,Cohen JL,Masurier C,Klatzmann D

MA5-17597 was used in flow cytometry to characterize different populations of mouse lymph node dendritic cells.

Thu Jan 15 00:00:00 UTC 1998

Immunologic memory induced by a glycoconjugate vaccine in a murine adoptive lymphocyte transfer model.

Infection and immunity

Guttormsen HK,Wetzler LM,Finberg RW,Kasper DL

MA5-17597 was used in flow cytometry to develop a mouse model to study the ability of a glycoprotein conjugate vaccine to induce immunologic memory for the polysaccharide moiety.

Fri May 01 00:00:00 UTC 1998

Bioinformatics

Protein Aliases: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

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Gene Aliases: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

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UniProt ID: (Mouse) P01731, (Mouse) P10300

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Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

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Function(s)

protein binding protein kinase binding protein homodimerization activity

Process(es)

immune system process T cell mediated immunity cell surface receptor signaling pathway T cell activation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus

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CD8 alpha Monoclonal Antibody (CT-CD8a), FITC

CD8 alpha Antibody (MA5-17597) in Flow

Product Details

MA5-17597

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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