Invitrogen

CD80 Monoclonal Antibody (16-10A1)

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CD80 Antibody (MA1-10287) in Flow

Separation of murine CD80 positive monocytes (red-filled) from CD80 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of mouse peritoneum fluid cells stained using anti-mouse CD80 (16-10A1) purified Monoclonal antibody (Product # MA1-10287) (concentration in sample 2 µg/mL) GAM APC.

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Product Details

MA1-10287

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Flow Cytometry (Flow)

1-4 µg/mL

View 8 publications 8 publications

Immunoprecipitation (IP)

Assay-Dependent

Blocking Assay (BLOCK)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG2

Class

Monoclonal

Type

Antibody

Clone

16-10A1

Immunogen

CD80-transfected CHO cell line

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11153728

Product Specific Information

This antibody reacts with an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Defective germinal center B-cell response and reduced arthritic pathology in microRNA-29a-deficient mice.

Cellular and molecular life sciences : CMLS

van Nieuwenhuijze A,Dooley J,Humblet-Baron S,Sreenivasan J,Koenders M,Schlenner SM,Linterman M,Liston A

MA110287 was used in flow cytometry to evaluate miR-29a in B cells as a potential therapeutic target in arthritis

Thu Jun 01 00:00:00 UTC 2017

Potentiation of T Cell Stimulatory Activity by Chemical Fixation of a Weak Peptide-MHC Complex.

Molecules and cells

Hwang I,Kim K,Choi S,Lomunova M

MA110287 was used in blocking or activating experiment to explore if the binding strength of a peptide for a MHC is a critical factor to determine the duration of pMHC-mediated T cell activation

Sun Jan 01 00:00:00 UTC 2017

CD40-signalling abrogates induction of RORγt⁺ Treg cells by intestinal CD103⁺ DCs and causes fatal colitis.

Nature communications

Barthels C,Ogrinc A,Steyer V,Meier S,Simon F,Wimmer M,Blutke A,Straub T,Zimber-Strobl U,Lutgens E,Marconi P,Ohnmacht C,Garzetti D,Stecher B,Brocker T

MA110287 was used in flow cytometry to report a CD40-dependent mechanism capable of abrogating inducible T regulatory cell induction by dendritic cells

Thu Mar 09 00:00:00 UTC 2017

TLR-mediated albuminuria needs TNFα-mediated cooperativity between TLRs present in hematopoietic tissues and CD80 present on non-hematopoietic tissues in mice.

Disease models & mechanisms

Jain N,Khullar B,Oswal N,Banoth B,Joshi P,Ravindran B,Panda S,Basak S,George A,Rath S,Bal V,Sopory S

MA110287 was used in flow cytometry to elucidate the mechanistic relationships among TLRs, CD80, and albuminuria

Wed Jun 01 00:00:00 UTC 2016

CXCL16-positive dendritic cells enhance invariant natural killer T cell-dependent IFNγ production and tumor control.

Oncoimmunology

Veinotte L,Gebremeskel S,Johnston B

MA110287 was used in flow cytometry to study the contribution of CXCR6/CXCL16 interactions in glycolipid-dependent invariant natural killer cell activation and tumor control

Wed Jun 01 00:00:00 UTC 2016

Lymphocytic Microparticles Modulate Angiogenic Properties of Macrophages in Laser-induced Choroidal Neovascularization.

Scientific reports

Tahiri H,Omri S,Yang C,Duhamel F,Samarani S,Ahmad A,Vezina M,Bussières M,Vaucher E,Sapieha P,Hickson G,Hammamji K,Lapointe R,Rodier F,Tremblay S,Royal I,Cailhier JF,Chemtob S,Hardy P

MA110287 was used in flow cytometry to study the ability of lymphocyte-derived microparticles to modulate the gene expression pattern of angiogenesis-related factors in macrophages

Tue Nov 22 00:00:00 UTC 2016

Peptide-MHC-I from Endogenous Antigen Outnumber Those from Exogenous Antigen, Irrespective of APC Phenotype or Activation.

PLoS pathogens

Sei JJ,Haskett S,Kaminsky LW,Lin E,Truckenmiller ME,Bellone CJ,Buller RM,Norbury CC

MA110287 was used in flow cytometry to parse the relative importance of the direct and indirect antigen presentation pathways in ectromelia virus mouse pox infection.

Mon Jun 01 00:00:00 UTC 2015

Combined immune checkpoint protein blockade and low dose whole body irradiation as immunotherapy for myeloma.

Journal for immunotherapy of cancer

Jing W,Gershan JA,Weber J,Tlomak D,McOlash L,Sabatos-Peyton C,Johnson BD

MA110287 was used in flow cytometry to test if blocking several checkpoint receptors boosts anti-tumor immunity in a low-dose, lymphodepleting whole body radiation model

Fri Jan 23 00:00:00 UTC 2015

Transgenic TCR expression: comparison of single chain with full-length receptor constructs for T-cell function.

Cancer gene therapy

Zhang T,He X,Tsang TC,Harris DT

MA1-10287 was used in flow cytometry to investigate the use of three-domain single-chain T-cell receptors

Thu Jul 01 00:00:00 UTC 2004

Targeted inactivation of the tetraspanin CD37 impairs T-cell-dependent B-cell response under suboptimal costimulatory conditions.

Molecular and cellular biology

Knobeloch KP,Wright MD,Ochsenbein AF,Liesenfeld O,Löhler J,Zinkernagel RM,Horak I,Orinska Z

MA1-10287 was used in flow cytometry to generate and characterize mice deficient for CD37.

Tue Aug 01 00:00:00 UTC 2000

Bioinformatics

Protein Aliases: Activation B7-1 antigen; B7; B7 protein; B7-1 protein; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); sCD 80; sCD80; soluble CD 80; soluble CD80; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1

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Gene Aliases: B7; B71; Cd28l; Cd80; Ly-53; Ly53; MIC17; TSA1

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UniProt ID: (Mouse) Q00609

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Entrez Gene ID: (Dog) 403765, (Mouse) 12519

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Function(s)

protein binding

Process(es)

T cell costimulation positive regulation of T cell proliferation positive regulation of alpha-beta T cell proliferation positive regulation of peptidyl-tyrosine phosphorylation cellular response to lipopolysaccharide

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD80 Monoclonal Antibody (16-10A1)

CD80 Antibody (MA1-10287) in Flow

Product Details

MA1-10287

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Blocking Assay (BLOCK)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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