Invitrogen

CD80 Monoclonal Antibody (MEM-233)

1 Published Figure

15 References

View all (187) CD80 (B7-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

FIGURE: 1 / 2

CD80 Antibody (MA1-19215) in Flow

Flow Cytometry analysis of CD80 using CD80 Monoclonal Antibody (MEM-233) (Product # MA1-19215). Separation of CD80 transfected P815 cells stained using anti-human CD80 (MEM-233) purified antibody (concentration in sample 1.67 μg/mL, GAM APC, red-filled) from CD80 transfected P815 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

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Product Details

MA1-19215

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1-4 µg/mL

View 12 publications 12 publications

Immunoprecipitation (IP)

Assay-dependent

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MEM-233

Immunogen

Extracellular domain of human CD80 fused to human IgG1(Fc).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1073446

Product Specific Information

This antibody reacts with an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

One, two or three? Probing the stoichiometry of membrane proteins by single-molecule localization microscopy.

Scientific reports

Fricke F,Beaudouin J,Eils R,Heilemann M

MA1-19215 was used in flow cytometry to learn about stoichiometry of membrane proteins by single-molecule localization microscopy

Fri Sep 11 00:00:00 UTC 2015

Susceptibility of human placenta derived mesenchymal stromal/stem cells to human herpesviruses infection.

PloS one

Avanzi S,Leoni V,Rotola A,Alviano F,Solimando L,Lanzoni G,Bonsi L,Di Luca D,Marchionni C,Alvisi G,Ripalti A

MA1-19215 was used in flow cytometry to assess the susceptibility of fetal membranes-derived mesenchymal stromal/stem cells to all members of the human Herpesviridae family.

Mon Apr 14 00:00:00 UTC 2014

Activated memory B cells may function as antigen-presenting cells in the joints of children with juvenile idiopathic arthritis.

Arthritis and rheumatism

Morbach H,Wiegering V,Richl P,Schwarz T,Suffa N,Eichhorn EM,Eyrich M,Girschick HJ

MA1-19215 was used in Flow Cytometry to investigate whether synovial B cells in juvenile idiopathic arthritis display features of antigen-presenting cells.

Tue Nov 01 00:00:00 UTC 2011

Activation of Fc gamma RI on monocytes triggers differentiation into immature dendritic cells that induce autoreactive T cell responses.

Journal of immunology (Baltimore, Md. : 1950)

Tanaka M,Krutzik SR,Sieling PA,Lee DJ,Rea TH,Modlin RL

MA1-19215 was used in flow cytometry to test if activation of monocytes via FcgammaR induced cell differentiation

Sat Aug 15 00:00:00 UTC 2009

Dendritic cell differentiation induced by a self-peptide derived from apolipoprotein E.

Journal of immunology (Baltimore, Md. : 1950)

Stephens TA,Nikoopour E,Rider BJ,Leon-Ponte M,Chau TA,Mikolajczak S,Chaturvedi P,Lee-Chan E,Flavell RA,Haeryfar SM,Madrenas J,Singh B

MA1-19215 was used in flow cytometry to identify and characterize a signal transduction cascade in monocytes that results in their differentiation into dendritic cells

Sat Nov 15 00:00:00 UTC 2008

A novel human B cell subpopulation representing the initial germinal center population to express AID.

Blood

Kolar GR,Mehta D,Pelayo R,Capra JD

MA1-19215 was used in flow cytometry to characterize B cells in human tonsils

Thu Mar 15 00:00:00 UTC 2007

Innate immunity modulates autoimmunity: type 1 interferon-beta treatment in multiple sclerosis promotes growth and function of regulatory invariant natural killer T cells through dendritic cell maturation.

Immunology

Gigli G,Caielli S,Cutuli D,Falcone M

MA1-19215 was used in flow cytometry to indicate that type 1 interferon-beta promotes the expansion and function of invariant natural killer cells for multiple sclerosis and type 1 diabetes treatment

Thu Nov 01 00:00:00 UTC 2007

LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lee DJ,Sieling PA,Ochoa MT,Krutzik SR,Guo B,Hernandez M,Rea TH,Cheng G,Colonna M,Modlin RL

MA1-19215 was used in flow cytometry to test if leukocyte Ig-like receptor A2 regulates DC differentiation using a leprosy model.

Sat Dec 15 00:00:00 UTC 2007

Flow cytometric analysis on cross-reactivity of human-specific CD monoclonal antibodies with splenocytes of Aotus nancymaae, a non-human primate model for biomedical research.

Veterinary immunology and immunopathology

Daubenberger CA,Spirig R,Patarroyo ME,Pluschke G

MA1-19215 was used in flow cytometry to utilize single color flow cytometry test to reactivity of 204 monoclonal antibodies against human cell surface molecules in Aotus nancymaae splenocytes

Sat Sep 15 00:00:00 UTC 2007

Immunophenotypic heterogeneity of bone marrow-derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment.

Haematologica

Campioni D,Moretti S,Ferrari L,Punturieri M,Castoldi GL,Lanza F

MA1-19215 was used in flow cytometry to use flow cytometry to characterize mesenchymal stromal cell subsets and abnormalities

Wed Mar 01 00:00:00 UTC 2006

Reduced IFN-alpha secretion by blood dendritic cells in human diabetes.

Clinical immunology (Orlando, Fla.)

Summers KL,Marleau AM,Mahon JL,McManus R,Hramiak I,Singh B

MA1-19215 was used in flow cytometry to characterize dendritic cells in type 1 and type 2 diabetes

Sun Oct 01 00:00:00 UTC 2006

Major histocompatibility complex class II (DR) antigen and costimulatory molecules on in vitro and in vivo activated human polymorphonuclear neutrophils.

Immunology

Sandilands GP,McCrae J,Hill K,Perry M,Baxter D

MA1-19215 was used in flow cytometry and immunocytochemistry to compare X-L of Mac -1 with other neutrophil activators

Fri Dec 01 00:00:00 UTC 2006

Acquisition of HLA-DR and costimulatory molecules by T cells from allogeneic antigen presenting cells.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Game DS,Rogers NJ,Lechler RI

MA1-19215 was used in flow cytometry to test if transfer of allogeneic material to T cells modifies human alloresponses

Fri Jul 01 00:00:00 UTC 2005

Cross-linking of neutrophil CD11b results in rapid cell surface expression of molecules required for antigen presentation and T-cell activation.

Immunology

Sandilands GP,Ahmed Z,Perry N,Davison M,Lupton A,Young B

MA1-19215 was used in flow cytometry to investigate how neutrophils present antigen

Tue Mar 01 00:00:00 UTC 2005

Cross-talk between activated human NK cells and CD4+ T cells via OX40-OX40 ligand interactions.

Journal of immunology (Baltimore, Md. : 1950)

Zingoni A,Sornasse T,Cocks BG,Tanaka Y,Santoni A,Lanier LL

MA1-19215 was used in flow cytometry to report that OX40 ligand is selectively induced by IL-2, IL-12, or IL-15-activated human NK cells following stimulation through NKG2D

Wed Sep 15 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: Activation B7-1 antigen; B-lymphocyte activation antigen B7; B7; BB1; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; sCD 80; sCD80; soluble CD 80; soluble CD80; T-lymphocyte activation antigen CD80; TS/A-1

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Gene Aliases: B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; CD80; LAB7

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UniProt ID: (Human) P33681

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Entrez Gene ID: (Human) 941

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Function(s)

virus receptor activity protein binding coreceptor activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity

Process(es)

cell-cell signaling positive regulation of signal transduction regulation of phosphatidylinositol 3-kinase signaling T cell costimulation intracellular signal transduction T cell activation positive regulation of interleukin-2 biosynthetic process positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process positive regulation of T-helper 1 cell differentiation positive regulation of transcription, DNA-templated positive regulation of alpha-beta T cell proliferation viral entry into host cell phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of peptidyl-tyrosine phosphorylation cellular response to lipopolysaccharide

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CD80 Monoclonal Antibody (MEM-233)

CD80 Antibody (MA1-19215) in Flow

Product Details

MA1-19215

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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