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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD80 (B7-1) Antibodies

          Invitrogen

          CD80 (B7-1) Monoclonal Antibody (16-10A1), PE-Cyanine5, eBioscience™

          60 Published Figures
          68 References
          View all (187) CD80 (B7-1) antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite CD80 (B7-1) Monoclonal Antibody (16-10A1), PE-Cyanine5, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (60)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 61

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          CD80 (B7-1) Antibody (15-0801-82) in Flow

          Staining of 3-day LPS activated C57Bl/6 splenocytes with 0.03 µg of Armenian Hamster IgG Isotype Control PE-Cyanine5 (Product # 15-4888-82) (blue histogram) or 0.03 µg of Anti-Mouse CD80 (B7-1) PE-Cyanine5 (purple histogram).Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

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          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)
          CD80 (B7-1) Antibody in Flow Cytometry (Flow)

          Product Details

          15-0801-82

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          0.06 µg/test
          View 68 publications 68 publications
          Product Specifications

          Species Reactivity

          Dog, Mouse, Pig

          Published species

          Mouse

          Host/Isotype

          Armenian hamster / IgG

          Recommended Isotype Control

          Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine5, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          16-10A1

          Conjugate

          PE-Cyanine5 PE-Cyanine5 PE-Cyanine5
          • Unconjugated
          • Alexa Fluor 700 
          • APC 
          • Biotin 
          • Brilliant UV 737
          • Brilliant Violet 480
          • Brilliant Violet 711
          • Brilliant Violet 786
          • eFluor 450
          • FITC
          • Functional Grade
          • PE 
          • PE-Cyanine7
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Request custom conjugation

          Excitation/Emission Max

          568/666 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_468774

          Product Specific Information

          Description: The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells and activated B cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively.

          Applications Reported: The 16-10A1 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: The 16-10A1 antibody has been tested by flow cytometric analysis of activated mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

          Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

          Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: Activation B7-1 antigen; B7; B7 protein; B7-1 protein; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); sCD 80; sCD80; soluble CD 80; soluble CD80; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1

          View more View less

          Gene Aliases: B7; B7-1; B71; Cd28l; Cd80; Ly-53; Ly53; MIC17; TSA1

          View more View less

          UniProt ID: (Mouse) Q00609

          View more View less

          Entrez Gene ID: (Pig) 397161, (Dog) 403765, (Mouse) 12519

          View more View less

          Function(s)
          protein binding
          Process(es)
          T cell costimulation positive regulation of T cell proliferation positive regulation of alpha-beta T cell proliferation positive regulation of peptidyl-tyrosine phosphorylation cellular response to lipopolysaccharide
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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